RESUMEN
New sampling format of whole cows' milk in strip-dried form was proposed. Few methodological issues of whole milk progesterone ELISA using samples dried on a membrane carrier in a form of strip were investigated and optimized: width of a strip, shape of punched/cut-off part of membrane, sample application method. It was shown that distribution of the hormone along narrow strip was even except the initial part of a strip (the first 0.5 × 0.5cm piece) where recovered concentration of progesterone was higher. Storage stability of progesterone in strip-dried whole cows' milk samples at 4°C, ambient temperature, 37°C and 60°C was investigated. Rising of the detected progesterone concentration over storage period at elevated temperatures was observed predominantly in milk samples with low hormone concentration (from non-pregnant cows). Strip-dried whole milk samples can be used for collection, transportation, storage and ELISA analysis of progesterone level which is correlated with reproductive status of cows.
Asunto(s)
Desecación/instrumentación , Ensayo de Inmunoadsorción Enzimática/instrumentación , Leche/química , Progesterona/análisis , Manejo de Especímenes/instrumentación , Animales , Bovinos , Femenino , Tiras Reactivas/análisis , TemperaturaRESUMEN
The dynamics of the erythrocyte sedimentation rate (ESR) in patients with ischemic disease shows a significantly different pattern in the days of raised geomagnetic activity as compared to quiet days. This is revealed by a greater intensity of ESR variations during geomagnetic storms as compared to the days of when there was little or no sun activity (quiet sun). Such behavior of red blood cells reflects the patient's individual sensitivity to geomagnetic field fluctuations. This article discusses a possible mechanism of ESR variations.