RESUMEN
A novel fatty acid binding protein, As-p18, is secreted into both the perivitelline and perienteric fluids of the parasitic nematode, Ascaris suum, and at least eight potential homologues of As-p18 have been identified in the Caenorhabditis elegans genome. The products of the three most closely related homologues are fatty acid binding proteins (LBP-1, LBP-2 and LBP-3) which contain putative secretory signals. Phylogenetic analysis revealed that these secreted fatty acid binding proteins comprise a distinct gene class within the fatty acid binding protein family and are possibly unique to nematodes. To examine the potential sites of As-p18 secretion, the expression of the putative promoters of the C. elegans homologues was examined with GFP reporter constructs. The developmental expression of lbp-1 was identical to that of As-p18 and consistent with the secretion of LBP-1 from the hypodermis to the perivitelline fluid. The expression patterns of lbp-2 and lbp-3 were consistent with the secretion of LBP-2 and LBP-3 from muscle into the perienteric fluid later in development. These studies demonstrate that at least some perivitelline fluid proteins appear to be secreted from the hypodermis prior to the formation of the cuticle and, perhaps more importantly, that this coordinate C. elegans/A. suum approach may be potentially useful for examining a number of key physiological processes in parasitic nematodes.
Asunto(s)
Ascaris suum/metabolismo , Caenorhabditis/metabolismo , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Proteínas del Helminto , Proteína P2 de Mielina/genética , Proteína P2 de Mielina/metabolismo , Proteínas de Neoplasias , Secuencia de Aminoácidos , Animales , Ascaris suum/genética , Ascaris suum/crecimiento & desarrollo , Caenorhabditis/genética , Caenorhabditis/crecimiento & desarrollo , Proteínas Portadoras/clasificación , Proteínas Portadoras/aislamiento & purificación , ADN de Helmintos/análisis , ADN de Helmintos/genética , Proteínas de Unión a Ácidos Grasos , Regulación del Desarrollo de la Expresión Génica , Genes de Helminto , Datos de Secuencia Molecular , Familia de Multigenes , Proteína P2 de Mielina/clasificación , Proteína P2 de Mielina/aislamiento & purificación , Filogenia , Alineación de Secuencia , Análisis de Secuencia de ADNRESUMEN
Pyruvate dehydrogenase kinases (PDKs) from the anaerobic parasitic nematode Ascaris suum and the free-living nematode Caenorhabditis elegans were functionally expressed with hexahistidine tags at their N-termini and purified to apparent homogeneity. Both recombinant PDKs (rPDKs) were dimers, were not autophosphorylated and exhibited similar specific activities with the A. suum pyruvate dehydrogenase (E1) as substrate. In addition, the activities of both PDKs were activated by incubation with PDK-depleted A. suum muscle pyruvate dehydrogenase complex (PDC) and were stimulated by NADH and acetyl-CoA. However, the recombinant A. suum PDK (rAPDK) required higher NADH/NAD+ ratios for half-maximal stimulation than the recombinant C. elegans PDK (rCPDK) or values reported for mammalian PDKs, as might be predicted by the more reduced microaerobic mitochondrial environment of the APDK. Limited tryptic digestion of both rPDKs yielded stable fragments truncated at the C-termini (trPDKs). The trPDKs retained their dimeric structure and exhibited substantial PDK activity with the A. suum E1 as substrate, but PDK activity was not activated by incubation with PDK-depleted A. suum PDC or stimulated by elevated NADH/NAD+ or acetyl-CoA/CoA ratios. Direct-binding assays demonstrated that increasing amounts of rCPDK bound to the A. suum PDK-depleted PDC. No additional rCPDK binding was observed at ratios greater than 20 mol of rCPDK/mol of PDC. In contrast, the truncated rCPDK (trCPDK) did not exhibit significant binding to the PDC. Similarly, a truncated form of rCPDK, rCPDK1-334, generated by mutagenesis, exhibited properties similar to those observed for trCPDK. These results suggest that the C-terminus of the PDK is not required for subunit association of the homodimer or catalysis, but instead seems to be involved in the binding of the PDKs to the dihydrolipoyl transacetylase core of the complex.
Asunto(s)
Acetiltransferasas/metabolismo , Ascaris suum/enzimología , Caenorhabditis elegans/enzimología , Proteínas Quinasas/metabolismo , Complejo Piruvato Deshidrogenasa/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Cartilla de ADN , Acetiltransferasa de Residuos Dihidrolipoil-Lisina , Hidrólisis , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Proteínas Quinasas/química , Proteínas Quinasas/genética , Proteínas Serina-Treonina Quinasas , Piruvato Deshidrogenasa Quinasa Acetil-Transferidora , Complejo Piruvato Deshidrogenasa/química , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Homología de Secuencia de AminoácidoRESUMEN
The pyruvate dehydrogenase complex (PDC) plays a key role in the anaerobic mitochondrial metabolism of the parasitic nematode Ascaris suum. A cDNA coding for an A. suum pyruvate dehydrogenase kinase (APDK) has been cloned and sequenced from poly(A)+ RNA isolated from adult A. suum muscle.2 APDK exhibited significant sequence identity to mammalian PDKs. Nucleotide sequence analysis of the APDK cDNA revealed a 22-nucleotide spliced leader, characteristic of many nematode mRNAs, a 5'-UTR of 6 nucleotides, an open reading frame of 1197 nucleotides, and a 3'-UTR of 101 nucleotides that included a putative polyadenylation signal. The open reading frame predicted a protein of 399 amino acids with a molecular weight of 45,402 that included a putative 18-aminoacid leader peptide. Recombinant APDK (rAPDK) was functionally expressed in Escherichia coli with a his tag at its N-terminus and purified to apparent homogeneity on Ni-NTA-agarose. Recombinant APDK was a dimer and was not autophosphorylated and its activity was stimulated in the presence of APDK-deficient adult A. suum muscle PDC presumably by the binding of APDK to the dihydrolipoyl transacetylase (E2) core of the complex. After binding to the core, rAPDK activity was stimulated by elevated NADH/NAD+ and acetyl CoA/CoA ratios within the same ranges as observed for the native APDK. Immunoblotting suggested that native APDK focused as a series of 43-kDa spots (pI 6.1-6.8) on two-dimensional gels of the purified adult A. suum muscle PDC.
Asunto(s)
Ascaris suum/enzimología , Músculos/enzimología , Proteínas Quinasas/biosíntesis , Secuencia de Aminoácidos , Anaerobiosis , Animales , Secuencia de Bases , Clonación Molecular , Secuencia de Consenso , Escherichia coli , Humanos , Intestinos/parasitología , Cinética , Datos de Secuencia Molecular , Peso Molecular , Sistemas de Lectura Abierta , Proteínas Quinasas/química , Proteínas Quinasas/genética , Proteínas Serina-Treonina Quinasas , Piruvato Deshidrogenasa Quinasa Acetil-Transferidora , Empalme del ARN , ARN de Helminto/metabolismo , ARN Mensajero/metabolismo , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , PorcinosRESUMEN
The 2-methyl branched-chain enoyl CoA reductase (ECR) plays a pivotal role in the reversal of beta-oxidation operating in anaerobic mitochondria of the parasitic nematode, Ascaris suum. Two-dimensional gel electrophoresis of the purified ECR yielded multiple spots, with two distinct but overlapping N-terminal sequences. These multiple isoforms were not the result of population effects, as the pattern observed on 2-D gels of the purified ECR was identical to those on immunoblots of muscle homogenates isolated from individual worms. A full-length cDNA coding for the major ECR isoform (ECRI) has been cloned and sequenced and compared with that of the minor isoform (ECRII) which has been described previously (Duran et al. J Biol Chem 1993;268:22391-22396). ECRI contained the 22-nucleotide trans-spliced leader sequence characteristic of many nematode mRNAs, a 5' untranslated region (UTR) of 13 nucleotides, an open reading frame (ORF) of 1257 nucleotides, a 3'-UTR of 110 nucleotides that included the polyadenylation signal AATAAA downstream of the termination codon and a short poly(A) tail. The ORF predicted a 16 amino acid leader sequence not found in the native protein and a mature protein of 403 amino acids with a molecular weight of 43 698 and a predicted pI of 6.2. ECRI and ECRII were 73% identical at the predicted amino acid level and their mRNAs exhibited significant structural similarity even though they were products of separate genes. Comparison of ECRI and ECRII with the sequences of acyl CoA dehydrogenases from a variety of different sources revealed a high degree of interspecies sequence identity, suggesting that these enzymes may have evolved from a common ancestral gene. This result is surprising since the ascarid enzymes function as reductases, not as dehydrogenases. Both ECRs were tissue-specific and developmentally regulated and were found in transitional third-stage larvae (L3) and adult muscle, but not in early, aerobic larval stages or adult testis, ovary, or intestine. The ratio of ECRII to ECRI was greater in L3 than in adult muscle. Interestingly, both ECRs also appeared to be expressed in pharyngeal muscle, suggesting that branched-chain fatty acid synthesis may not be confined exclusively to body wall muscle.
Asunto(s)
Ascaris suum/enzimología , Regulación del Desarrollo de la Expresión Génica , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH , Oxidorreductasas/genética , Secuencia de Aminoácidos , Animales , Ascaris suum/genética , Ascaris suum/crecimiento & desarrollo , Secuencia de Bases , Clonación Molecular , Electroforesis , Interacciones Huésped-Parásitos , Immunoblotting , Estadios del Ciclo de Vida , Mitocondrias Musculares/enzimología , Datos de Secuencia Molecular , Oxidorreductasas/química , Oxidorreductasas/metabolismo , Músculos Faríngeos/enzimología , Alineación de SecuenciaRESUMEN
Rabbit lung-derived third-stage larvae (L3) of Ascaris suum are aerobic and cyanide sensitive but also contain many enzymes specific to anaerobic pathways. To localize these enzymes, diaminobenzidine (DAB) staining for cytochrome oxidase (COX) and immunogold labeling for 2-methylbutyryl enoyl CoA reductase (ECR) were performed on sections of hypodermis and muscle of adults and larvae of A. suum and visualized by transmission electron microscopy. As predicted, adult hypodermal and muscle mitochondria did not exhibit COX staining; however, hypodermal and muscle mitochondria of the L3 and fourth-stage larvae (L4) were DAB positive. In contrast, hypodermal mitochondria from the adult, L3, and L4 did not exhibit ECR immunoreactivity, whereas mitochondria from muscle of all 3 were ECR positive. These observations suggest that both the ECR and COX are colocalized in muscle mitochondria of the L3.
Asunto(s)
Ascaris suum/enzimología , Complejo IV de Transporte de Electrones/análisis , Ácido Graso Desaturasas/análisis , 3,3'-Diaminobencidina , Animales , Ascaris suum/ultraestructura , Femenino , Inmunohistoquímica , Larva/enzimología , Microscopía Electrónica , Mitocondrias Musculares/enzimología , Conejos , Coloración y EtiquetadoRESUMEN
Early development of the parasitic nematode, Ascaris suum, occurs inside a highly resistant eggshell, and the developing larva is bathed in perivitelline fluid. Two-dimensional gel analysis of perivitelline fluid from infective larvae reveals seven major proteins; a cDNA encoding one of these, As-p18, has been cloned, sequenced, and protein expressed in Escherichia coli. The predicted amino acid sequence of As-p18 exhibits similarities to the intracellular lipid-binding protein (iLBP) family including retinoid- and fatty acid-binding proteins (FABP). As-p18 is unusual in that it possesses a hydrophobic leader that is not present in the mature protein, the developmental regulation of its expression, and in terms of its predicted structure. Recombinant As-p18 is a functional FABP with a high affinity for both a fluorescent fatty acid analog (11(((5-(dimethylamino)-1-naphthalenyl)sulfonyl)amino) undecanoic acid) and oleic acid, but not retinol. Circular dichroism of rAs-p18 reveals a high beta-sheet content (62%), which is consistent with secondary structure for the protein predicted from sequence algorithms, and the structure of iLBPs. Unusual features are apparent in a structural model of As-p18 generated from existing crystal structures of iLBPs. As-p18 is not found in unembryonated eggs, begins to be synthesized at about day 3 of development, reaches a maximal concentration with the formation of the first-stage larva and remains abundant in the perivitelline fluid of the second-stage larva. Since As-p18 is not present in the post-infective third-stage larva or adult worm tissues, it appears to be exclusive to the egg. Surprisingly, however, Northern blot analysis yields mRNA for As-p18 not only in the early larval stages, but also the unembryonated egg, third-stage larvae, and ovaries of adult worms, even though the protein is not detectable from any of those sources. As-p18 may play a role in sequestering potentially toxic fatty acids and their peroxidation products, or it may be involved in the maintenance of the impermeable lipid layer of the eggshell.
Asunto(s)
Proteínas Portadoras/metabolismo , Ácidos Grasos/metabolismo , Proteínas del Helminto , Proteína P2 de Mielina/metabolismo , Proteínas de Neoplasias , Proteínas del Tejido Nervioso , Membrana Vitelina/química , Secuencia de Aminoácidos , Animales , Ascaris suum , Proteínas Portadoras/química , Proteínas Portadoras/genética , Bovinos , Dicroismo Circular , Electroforesis en Gel Bidimensional , Proteína de Unión a los Ácidos Grasos 7 , Proteínas de Unión a Ácidos Grasos , Ratones , Modelos Moleculares , Datos de Secuencia Molecular , Proteína P2 de Mielina/química , Conformación Proteica , Conejos , Proteínas Recombinantes/metabolismo , Espectrometría de FluorescenciaRESUMEN
A novel dihydrolipoyl dehydrogenase-binding protein (E3BP) which lacks an amino-terminal lipoyl domain, p45, has been identified in the pyruvate dehydrogenase complex (PDC) of the adult parasitic nematode, Ascaris suum. Sequence at the amino terminus of p45 exhibited significant similarity with internal E3-binding domains of dihydrolipoyl transacetylase (E2) and E3BP. Dissociation and resolution of a pyruvate dehydrogenase-depleted adult A. suum PDC in guanidine hydrochloride resulted in two E3-depleted E2 core preparations which were either enriched or substantially depleted of p45. Following reconstitution, the p45-enriched E2 core exhibited enhanced E3 binding, whereas, the p45-depleted E2 core exhibited dramatically reduced E3 binding. Reconstitution of either the bovine kidney or A. suum PDCs with the A. suum E3 suggested that the ascarid E3 was more sensitive to NADH inhibition when bound to the bovine kidney core. The expression of p45 was developmentally regulated and p45 was most abundant in anaerobic muscle. In contrast, E3s isolated from anaerobic muscle or aerobic second-stage larvae were identical. These results suggest that during the transition to anaerobic metabolism, E3 remains unchanged, but it appears that a novel E3BP, p45, is expressed which may help to maintain the activity of the PDC in the face of the elevated intramitochondrial NADH/NAD+ ratios associated with anaerobiosis.
Asunto(s)
Ascaris suum/enzimología , Proteínas Portadoras/química , Proteínas Portadoras/metabolismo , Dihidrolipoamida Deshidrogenasa/metabolismo , Proteínas del Helminto/química , Proteínas del Helminto/metabolismo , Complejo Piruvato Deshidrogenasa/química , Complejo Piruvato Deshidrogenasa/metabolismo , Secuencia de Aminoácidos , Anaerobiosis , Animales , Sitios de Unión , Proteínas Portadoras/aislamiento & purificación , Electroforesis en Gel de Poliacrilamida , Flavina-Adenina Dinucleótido/metabolismo , Proteínas del Helminto/aislamiento & purificación , Cinética , Larva , Datos de Secuencia Molecular , NAD/metabolismo , Oxidación-Reducción , Homología de Secuencia de AminoácidoRESUMEN
Mitochondrial DNA (mtDNA) is finding increasing usage as a tool for studying the systematics, population genetics and epidemiology of parasitic helminths, and is generally assumed to be inherited maternally. Yet two features of Ascaris biology--fertilization by large amoeboid sperm and some novel aspects of sperm mitochondria--suggest a paternal component to mitochondrial inheritance in this organism. In this study, we compare mtDNA restriction patterns of parental worms with those of their progeny but find no evidence for paternal inheritance. We suggest that sperm-derived mitochondria are actively destroyed or outcompeted by maternal organelles in the zygote.
Asunto(s)
Ascaris/genética , ADN de Helmintos/genética , ADN Mitocondrial/genética , Impresión Genómica , Animales , Ascaris/fisiología , Secuencia de Bases , Cartilla de ADN , ADN de Helmintos/aislamiento & purificación , ADN Mitocondrial/aislamiento & purificación , Femenino , Masculino , Mitocondrias Musculares/fisiología , Datos de Secuencia Molecular , Mapeo Restrictivo , Cigoto/fisiologíaRESUMEN
The 2-methyl branched-chain enoyl-CoA reductase plays a pivotal role in the reversal of beta-oxidation operating in anaerobic mitochondria of the parasitic nematode Ascaris suum. An affinity-purified polyclonal anti-serum against the reductase was used to screen a cDNA library constructed in lambda gt11 with poly(A)+ RNA from adult A. suum muscle. A 1.2-kilobase partial cDNA clone was isolated, subcloned, and sequenced in both directions. Additional sequence at the 5' end of the mRNA was determined by the RACE (rapid amplification of cDNA ends) procedure. Nucleotide sequence analysis of the cDNAs revealed the 22-nucleotide trans-spliced leader sequence characteristic of many nematode mRNAs, an open reading frame of 1236 nucleotides and a 3'-untranslated sequence of 109 nucleotides including a short poly(A) tail 14 nucleotides from a polyadenylation signal (AATAAA). The open reading frame encoded a 396-amino acid sequence (M(r) 43,046) including a 16-amino acid leader peptide. Two-dimensional gel electrophoresis of the purified reductase yielded multiple spots with two distinct but overlapping amino-terminal amino acid sequences. Both sequences overlapped with the sequence predicted from the mRNA, and one of the sequences was identical to the predicted sequence. Comparison of the ascarid sequence with that of mammalian acyl-CoA dehydrogenases revealed a high degree of sequence identity, suggesting that these enzymes may have evolved from a common ancestral gene even though the ascarid enzyme functions as a reductase, not as a dehydrogenase. Immunoblotting of A. suum larval stages and adult tissues with antisera that cross-reacted with each of the spots separated on two-dimensional gels suggested that the reductase was only found in adult muscle. Northern blotting using the partial cDNA revealed a hybridization band of about 1.5 kilobases and also suggested that the enzyme was tissue-specific and developmentally regulated in agreement with the results of the immunoblotting.
Asunto(s)
Ascaris suum/enzimología , Mitocondrias/enzimología , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH , Oxidorreductasas/genética , Secuencia de Aminoácidos , Anaerobiosis , Animales , Ascaris suum/genética , Secuencia de Bases , Clonación Molecular , Secuencia de Consenso , ADN/genética , ADN/aislamiento & purificación , Cartilla de ADN , ADN Complementario/metabolismo , Ácido Graso Desaturasas/genética , Biblioteca de Genes , Humanos , Datos de Secuencia Molecular , Oligonucleótidos Antisentido , Poli A/metabolismo , ARN/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Homología de Secuencia de AminoácidoRESUMEN
The anaerobic metabolism of Ascaris suum body wall muscle mitochondria has been well characterized, but little is known about the metabolism of other adult tissues. The present study was designed to further characterize the metabolism of mitochondria isolated from A. suum male reproductive tissues, which contain predominately sperm, and to compare it with that of muscle. Cytochrome oxidase activity could not be detected in muscle, testis, or sperm mitochondria either by diaminobenzidine staining or enzymatic assays. However, the activities of several tricarboxylic acid cycle enzymes, including citrate synthase and isocitrate dehydrogenase, were about 100-fold higher in testis/seminal vesicle mitochondria than muscle mitochondria. In contrast, malic enzyme activity in testis/seminal vesicle mitochondria was about 12-fold lower than that in muscle mitochondria. The incorporation of 32Pi into organic phosphate by either muscle or testis/seminal vesicle mitochondria appeared to be dependent on malate and pyruvate, and incorporation was inhibited by rotenone but not cyanide. Thus, the metabolism of testis/seminal vesicle mitochondrial preparations appears to be similar to that of ascarid muscle, despite the elevated levels of tricarboxylic acid cycle enzyme activities present in testis/seminal vesicle mitochondria. The function of these elevated enzymes is unclear, but the possibility that they are used later in the aerobic metabolism of the fertilized egg has not been excluded.
Asunto(s)
Ascaris suum/ultraestructura , Mitocondrias/metabolismo , Animales , Ascaris suum/química , Ascaris suum/metabolismo , Fraccionamiento Celular , Ciclo del Ácido Cítrico , Complejo IV de Transporte de Electrones/metabolismo , Proteínas del Helminto/análisis , Malato Deshidrogenasa/metabolismo , Masculino , Microscopía Electrónica , Mitocondrias/química , Mitocondrias/ultraestructura , Mitocondrias Musculares/química , Mitocondrias Musculares/metabolismo , Mitocondrias Musculares/ultraestructura , Fosfatos/metabolismo , Fosforilación , Espermatozoides/metabolismo , Espermatozoides/ultraestructura , Testículo/metabolismo , Testículo/ultraestructuraRESUMEN
1. Catalase activity was partially purified from body wall muscle of the parasitic nematode, Ascaris suum, and was similar to catalases isolated from mammalian tissues. It exhibited a broad pH optimum and was unaffected by 2 mM ethylenediaminetetra-acetate. In contrast, it was inhibited reversibly by 1 mM cyanide and irreversibly by prior incubation in 40 mM 3-amino-1:2:4-triazole for 1 hr or heating at 80 degrees C for 15 min. 2. Catalase activity was highest in the unembryonated "egg" and decreased dramatically as development proceeded. 3. Catalase activity in adult body wall muscle was similar to that in rat skeletal muscle, but dramatically lower than that in rat liver. Catalase activity was barely detectable in A. suum testis. 4. Cytochrome-c peroxidase activity did not appear to be present in adult A. suum muscle mitochondria.
Asunto(s)
Ascaris/enzimología , Catalasa/metabolismo , Animales , Ascaris/crecimiento & desarrollo , Citocromo-c Peroxidasa/metabolismo , Femenino , Larva/enzimología , Masculino , Músculos/enzimología , Oxígeno/metabolismo , Fracciones Subcelulares/enzimologíaRESUMEN
The in vitro metabolism of [1-13C]glucose by Ascaris suum third and fourth-stage larvae was analyzed under different gas phases using 13C nuclear magnetic resonance spectroscopy (13C-NMR). Third-stage larvae (L3) incubated under a gas phase of 85% N2/5% O2/10% CO2 produced trace amounts of [13C]succinate, and molted to fourth-stage larvae (L4) between days 3 and 4 in vitro. However, they appeared to arrest as L3s when incubated under air, or 85% N2/5% O2/10% CO2 in the presence of 2 mM potassium cyanide, or 95% N2/5% CO2. Day 12 L4 (eight days after molting) incubated under 85% N2/5% O2/10% CO2, or 95% N2/5% CO2, or 94% N2/1% O2/5% CO2, produced succinate, acetate, propionate and the branched-chain fatty acids 2-methylvalerate and 2-methylbutyrate by fermentative pathways characteristic of adult body wall muscle. In contrast, when Day 12 L4 were incubated under air, only trace amounts of these acids were detected in the incubation medium. Thus, L4 are capable of synthesizing end-products typical of the adult even in the presence of oxygen, as long as the CO2 tensions are above 5%. As would be predicted, activities of enzymes involved in aerobic metabolism, including citrate synthase, isocitrate dehydrogenase, and cytochrome oxidase, decreased dramatically as L4s underwent the final ecdysis and matured to the adult stage. More importantly, activities of enzymes typical of anaerobic metabolism, including phosphoenolpyruvate carboxykinase and malic enzyme, were substantially elevated in L3s (over their levels in second-stage larvae), and appeared to have reached their adult levels in L3s prior to the third molt, even though L3s still exhibited cyanide sensitivity. Since L3s and L4s have enzymes involved in both aerobic and anaerobic pathways, it is possible that the L3s contain two populations of mitochondria, one which functions aerobically and a second which functions anaerobically.
Asunto(s)
Ascaris/metabolismo , Dióxido de Carbono/fisiología , Glucosa/metabolismo , Oxígeno/fisiología , Animales , Ascaris/efectos de los fármacos , Larva/efectos de los fármacos , Larva/metabolismo , Pulmón/parasitología , Espectroscopía de Resonancia Magnética , Nitrógeno/fisiología , Especificidad de Órganos , ConejosRESUMEN
The effects of inhibitors of protein synthesis and electron transport on the incorporation of [14C]leucine and [35S]methionine into protein by the filarial worm Brugia pahangi have been investigated. Cycloheximide inhibits the accumulation of both [14C]leucine and [35S]methionine by the worms and their incorporation into protein. In addition, inhibitors of electron transport and some anti-parasitic compounds also significantly inhibit filarial protein synthesis. Antimycin A and cyanide inhibit [14C]leucine incorporation into protein 63 and 72%, respectively, without affecting either motility or lactate production. Interestingly, the anti-malarial compounds chloroquine and quinacrine also significantly inhibit both accumulation and incorporation of [14C]leucine by B. pahangi. In addition, fluorographs of sodium dodecyl sulfate-polyacrylamide gels of homogenates from filariids incubated in [35S]methionine and cycloheximide with and without chloramphenicol indicate that there is a discrete population of proteins, possibly mitochondrial in origin, that are synthesized in the presence of cycloheximide and are not inhibited by chloramphenicol.
Asunto(s)
Brugia/efectos de los fármacos , Transporte de Electrón/efectos de los fármacos , Biosíntesis de Proteínas , Inhibidores de la Síntesis de la Proteína/farmacología , Animales , Antimicina A/farmacología , Cloranfenicol/farmacología , Cloroquina/farmacología , Cianuros/farmacología , Cicloheximida/farmacología , Lactatos/biosíntesis , Peso Molecular , Movimiento/efectos de los fármacos , Quinacrina/farmacologíaRESUMEN
Ascaris suum L3 larvae isolated from rabbit lungs undergo the third ecdysis to L4 larvae after 3 days in culture under a gas phase of 85% N2/10% CO2/5% O2. The L3 larvae contain substantial malic enzyme activity and are capable of producing small amounts of the reduced organic acids characteristic of the fermentative pathways which operate in the adult. However, only a small portion of the total carbon utilized is accounted for by these reduced acids and their motility is cyanide-sensitive, suggesting that their energy-generating pathways are predominantly aerobic. In contrast, after ecdysis, the L4 larvae begin to utilize glucose at a greater rate and the proportion of total carbon utilized which is accounted for as propionate, 2-methylbutyrate and 2-methylvalerate also increases. In addition, motility becomes increasingly cyanide-insensitive, suggesting that these L4 larvae are able to utilize the anaerobic energy-generating pathways of the adult. Surprisingly, on day 10 in culture, these L4 larvae, although capable of producing reduced volatile acids, still retain substantial cyanide-sensitive cytochrome oxidase activity.
Asunto(s)
Ascaris/metabolismo , Ácidos Grasos Volátiles/biosíntesis , Aerobiosis , Anaerobiosis , Animales , Ascaris/enzimología , Ascaris/crecimiento & desarrollo , Butiratos/biosíntesis , Cianuros/farmacología , Complejo IV de Transporte de Electrones/metabolismo , Metabolismo Energético , Glucosa/metabolismo , Larva/enzimología , Larva/metabolismo , Malato Deshidrogenasa/metabolismo , Movimiento/efectos de los fármacos , Oxidación-Reducción , Ácidos Pentanoicos/biosíntesis , Propionatos/biosíntesisRESUMEN
Glycogen synthase in skeletal muscle of 3-day alloxan-diabetic rats was found to be in a less active state than in normal muscle. Both the activity ratio (activity without G6P divided by activity with 7.2 mM G6P at 4.4 mM UDPG, pH 7.8) and fractional velocity (activity with 0.25 mM G6P divided by activity with 10 mM G6P at 0.03 mM UDPG, pH 6.9) were significantly lower in the diabetic tissue. Correspondingly, the S0.5 for UDPG and A0.5 for G6P were significantly higher in diabetic tissue, suggesting decreased affinity for substrate and activator, respectively. The kinetic changes in the diabetic synthase were identical whether the alloxan-treated animals were maintained on insulin for 7 days prior to withdrawal for 3 days, or studied 3 days immediately after alloxan treatment. The diabetes-induced changes in synthase could be reversed by injecting the diabetic rat with insulin 10 min prior to sacrifice. After insulin treatment, the S0.5 for UDPG and A0.5 for G6P decreased to control levels or lower and the activity ratios and fractional velocities increased to control levels or higher. The activity of glycogen synthase phosphatase was not decreased in diabetic skeletal muscle. This observation, coupled with the rapid response of the diabetic synthase to in vivo insulin treatment, suggests that, unlike the phosphatase in cardiac muscle and liver, the glycogen synthase phosphatase in skeletal muscle is not altered by the diabetic state.
Asunto(s)
Diabetes Mellitus Experimental/enzimología , Glucógeno Sintasa/metabolismo , Animales , Activación Enzimática/efectos de los fármacos , Glucofosfatos/farmacología , Glucógeno Sintasa-D Fosfatasa/metabolismo , Insulina/farmacología , Músculos/enzimología , Ratas , Uridina Difosfato Glucosa/farmacologíaRESUMEN
The effects of the anthelmintic drug, levamisole, on glycogen metabolism in the adult filariid, Litomosoides carinii, were examined. Incubation of helminths for up to 6 hr in the presence of levamisole resulted in a fourfold increase in total worm glycogen levels. In accord with this observation, levamisole also produced an eightfold increase in the rate of incorporation of [14C]-glucose into [14C]-glycogen over a 6-hr incubation period. In contrast, levamisole had no significant effect on 14CO2 evolution or lactate formation from exogenous glucose indicating that glycolysis was not affected. Because levamisole stimulated the incorporation of [14C]-glucose into glycogen, its effects on glycogen synthase activity were determined. Glycogen synthase in L. carinii was found to exist in both glucose-6-phosphate dependent (D) and glucose-6-phosphate independent (I) forms. In vitro incubation of intact L. carinii for 2 hr in the presence of levamisole resulted in a significant increase in the activity ratio (activity in the absence of glucose-6-P divided by the activity in the presence of glucose-6-P). This suggests that levamisole may act to stimulate the conversion of the less active (D) form of the glycogen synthase to the more active (I) form.
Asunto(s)
Filarioidea/efectos de los fármacos , Glucosa/metabolismo , Glucógeno Sintasa/metabolismo , Glucógeno/metabolismo , Levamisol/farmacología , Animales , Dióxido de Carbono/metabolismo , Filarioidea/metabolismo , Lactatos/metabolismo , Ácido LácticoRESUMEN
Disrupted Ascaris mitochondria formed 2-methylbutyrate (2-MB) and 2-methylvalerate (2-MV) when incubated anaerobically with acetyl CoA, propionyl CoA and NADH. However, when mitochondrial membranes were removed by high speed centrifugation and the mitochondrial soluble fraction was incubated with the same substrates, 2-methylcrotonate (tiglate) and a compound tentatively identified as 2-methyl-2- pentenoate accumulated rather than 2-MB or 2-MV. These data suggest that the terminal reduction of the unsaturated intermediates to the saturated 2-MB and 2-MV was catalyzed by an enzyme system at least partially bound to membranes. This supposition was further supported by the findings that disrupted Ascaris mitochondria also formed 2-MB and lesser amounts of 2-MV when incubated with tiglyl CoA plus NADH, and both soluble and membrane-bound components appear to be involved in this reduction. The possibility that electron transport associated ATP synthesis may be coupled to these reductions remains to be examined.
Asunto(s)
Ascaris/metabolismo , Ácidos Grasos Volátiles/biosíntesis , Mitocondrias Musculares/metabolismo , Acilcoenzima A , Animales , Ascaris/ultraestructura , Butiratos/biosíntesis , Sistema Libre de Células , Crotonatos/biosíntesis , Femenino , Flavina-Adenina Dinucleótido/farmacología , Hemiterpenos , NAD/metabolismo , Oxidación-Reducción , Ácidos Pentanoicos/biosíntesisRESUMEN
The rate of 14CO2 evolution from 1-[14C]pyruvate by intact Ascaris mitochondria was very slow, but increased with increasing concentrations of pyruvate. At all concentrations of pyruvate, in an aerobic environment, pyruvate decarboxylation was stimulated greatly by the addition of fumarate, malate, or succinate. However, under anaerobic conditions, only malate and fumarate stimulated pyruvate decarboxylation; succinate had no effect. This implies that the aerobic metabolism of succinate, presumably to other dicarboxylic acids, may be required for the stimulation. Incubation of sonicated mitochondria with pyruvate plus fumarate, under rate-limiting concentrations of NAD+, resulted in approximately equal quantities of pyruvate utilized and succinate formed, suggesting that pyruvate oxidation and fumarate reduction may be linked. Branched-chain, volatile fatty acids were not formed during incubations with either malate or succinate, or succinate plus acetate. However, incubations of intact Ascaris mitochondria with pyruvate plus succinate yielded 2-methylbutyrate and 2-methylvalerate, whereas incubations with pyruvate plus propionate yielded almost exclusively 2-methylvalerate. Oxygen dramatically inhibited the synthesis of the branched-chain acids from succinate plus pyruvate, attesting to the apparent anaerobic nature of Ascaris mitochondrial metabolism. Significantly, the addition of glucose plus ADP stimulated the formation of all volatile fatty acids. Therefore, the synthesis of branched-chain acids may be related directly to increased energy generation. Alternatively, they may function in the regulatory role of maintaining the mitochondrial redox balance.