RESUMEN

Bacteriochlorophyll(BChl)-f which has not yet been found in natural phototrophs was prepared by chemically modifying chlorophyll-b. The retention time of reverse-phase high-performance liquid chromatography of the synthetic monomeric BChl-f as well as its visible absorption and fluorescence emission spectra in a solution were identified and compared with other naturally occurring chlorophyll pigments obtained from the main light-harvesting antenna systems of green sulfur bacteria, BChls-c/d/e. Based on the above data, BChl-f was below the level of detection in three strains of green photosynthetic bacteria producing BChl-e.

Asunto(s)

Bacterioclorofilas/biosíntesis , Chlorobi/metabolismo , Bacterioclorofilas/síntesis química , Bacterioclorofilas/química , Clorofila/química , Clorofila/aislamiento & purificación , Cromatografía Líquida de Alta Presión , Spinacia oleracea/química