RESUMEN
Some evidence exists that cow body condition score (BCS) is associated with risks to animal health, and that higher BCS in extensively kept animals provides a buffer against cold conditions or other adverse weather events. Not surprisingly, measures of BCS feature in dairy cattle welfare assessment protocols. However, the degree to which BCS predicts welfare state, particularly in relation to the level of "hunger" experienced, is not well researched. The aim of this study was to examine associations between naturally arising variations in BCS in dairy cattle and time spent engaged in activities used as proxy indicators of hunger. Holstein-Friesian cows (n=113) of either North American or New Zealand ancestry were allocated to 1 of 3 levels of concentrate-feed supplementation (0, 3, or 6 kg of dry matter/d) and also offered pasture in excess of requirements. Body condition score (1 to 10 scale), pasture dry matter intake (DMI), and time budgets for grazing, ruminating, standing, and lying were recorded during early, mid, and late lactation. Body condition score varied over a wide range (2.5 to 8.5) and, within genetic strain and supplementation level, was inversely associated with pasture DMI, rate of DMI, and the proportions of time spent grazing and ruminating. In comparison, variation in BCS (within genetic strain and supplementation level) was directly associated with variations in time spent lying (in late lactation). Nevertheless, pasture DMI and time spent in the key behavioral activities were all within the normal expected limits for pasture-fed dairy cows. Thus, thin cows appeared able to achieve their nutritional requirements. Furthermore, even though thinner cows traded-off a small portion of their lying time in late lactation to forage longer, they still rested for normal periods each day. Based on these results, we found no difference in the welfare status of naturally thin and fat cows when they were offered generous pasture allowances with or without concentrate supplementation. The extent to which this pertains under conditions of persistently low feed allowances that result in reductions in BCS remains to be determined.
Asunto(s)
Bienestar del Animal , Bovinos , Animales , Conducta Animal , Bovinos/anatomía & histología , Bovinos/fisiología , Industria Lechera/métodos , Industria Lechera/normas , Dieta/veterinaria , FemeninoRESUMEN
Understanding how dairy cows adjust their grazing behavior in response to feed supplements is important for the development of management strategies that optimize profit from supplementation. New Zealand Holstein-Friesian (HF) cows have been selected for milk production on a predominantly pasture-based diet; in comparison, HF cows of North American (NA) ancestry have been selected almost exclusively for milk yield and fed diets high in nonfiber carbohydrates (NFC). We hypothesized, therefore, that supplementation would have differing effects on grazing behavior, pasture dry matter intake (DMI), and milk production in these genetic strains at peak, mid, and late lactation. A study was conducted over 2 consecutive lactations, with NA and NZ cows randomly allocated at calving to 0, 3, or 6 kg of dry matter/day concentrate plus unrestricted access to pasture. Pasture DMI, milk production, and grazing behavior were recorded at peak, mid, and late lactation. Concentrates were fed in equal amounts at morning and afternoon milking. The NA cows produced more milk and milk components, and had a greater pasture DMI, despite spending less time grazing. Declines in time spent grazing and pasture DMI were associated with increasing concentrate DMI. Grazing behavior following morning supplementation was different from that recorded following afternoon supplementation. Grazing ceased following morning supplementation before rumen fill could be a limiting factor, and the length of the grazing interval was inversely proportional to the amount of concentrate offered; these results suggest that physiological rather than physical stimuli were responsible for grazing cessation. The decrease in time spent grazing with increasing concentrate DMI is consistent with changes in neuroendocrine factors secreted in response to the presence of food in the digestive tract or with circulating products of digestion. After afternoon supplementation, sunset signaled the end of grazing irrespective of stage of lactation, timing of sunset, or supplementation status, suggesting that photoperiod influenced grazing behavior. Results confirmed changes in grazing behavior, an associated reduction in pasture DMI, and an increase in milk production when cows consume increasing amounts of concentrates. However, as the effect of supplement on grazing behavior differed between morning and afternoon supplementation, further research is required to better understand the factors controlling grazing behavior, to allow improved milk production responses to supplementary feeding.
Asunto(s)
Bovinos/fisiología , Dieta/veterinaria , Ingestión de Alimentos/fisiología , Conducta Alimentaria/fisiología , Lactancia/fisiología , Leche/metabolismo , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Bovinos/genética , Suplementos Dietéticos , Ingestión de Alimentos/genética , Femenino , Lactancia/genéticaRESUMEN
Fifty-six genetically divergent New Zealand and North American Holstein-Friesian (HF) cows grazed pasture, and were offered 0, 3, or 6 kg of concentrate DM/cow per day for an extended lactation (605 +/- 8.3 d in milk; mean +/- standard error of the mean). Weekly blood samples collected from individual cows from wk 1 to 10 postpartum (early lactation), and from wk 47 to 63 postpartum (extended lactation) were analyzed for nonesterified fatty acids (NEFA), glucose, insulin, leptin, growth hormone (GH), insulin-like growth factor-I (IGF-I), calcium, and urea. During early lactation, NEFA and GH concentrations were greater and IGF-I concentrations were less, and increased at a slower rate in North American HF. During this 10-wk period, there were no strain effects on plasma glucose, leptin, insulin, or calcium. During the extended lactation period, North American HF had greater NEFA and GH concentrations; there were strain x diet interactions for insulin and leptin, and a tendency for a strain x diet interaction for glucose. These interactions were primarily due to greater plasma insulin, leptin, and glucose concentrations in the New Zealand HF fed 6 kg of concentrate DM/cow per day, a result of excessive body condition in this treatment. In this period, there was no strain effect on plasma IGF-I, calcium, or urea concentration. During early lactation, there was a linear increase in glucose and IGF-I, and a linear decrease in GH and urea with increasing concentrate in the diet. However, plasma calcium, NEFA, insulin, and leptin remained unchanged. During the extended lactation period, there was an effect of feed supplementation on GH and urea, which decreased linearly with increasing concentrate in the diet. There was, however, no supplementation effect on NEFA, calcium, or IGF-I. These data indicate potential strain differences in recoupling of the somatotropic axis, insulin resistance, and energy partitioning, and may help explain the physiology behind the previously reported greater milk production and body condition score loss in North American HF. The results have implications for breeding and diet management during an extended lactation.
Asunto(s)
Bovinos/sangre , Bovinos/fisiología , Industria Lechera , Dieta/veterinaria , Lactancia/fisiología , Animales , Glucemia/análisis , Cruzamiento , Calcio/sangre , Bovinos/genética , Bovinos/metabolismo , Ácidos Grasos no Esterificados/sangre , Femenino , Hormona del Crecimiento/sangre , Insulina/sangre , Factor I del Crecimiento Similar a la Insulina/análisis , Leptina/sangre , Factores de Tiempo , Urea/sangreRESUMEN
The production of progesterone during the luteal phase of the estrous cycle regulates early embryonic development, uterine function, and luteal function. Plasma progesterone concentrations were measured during a spontaneous estrous cycle in lactating Holstein-Friesian cows of New Zealand or North American ancestry, fed either fresh pasture or a total mixed ration. Subpopulations of profiles were identified using 2 approaches: 1) shape-based clustering and 2) trait-based clustering. Subpopulations of profiles were compared using key progesterone profile components, which described the early and late luteal phase of the estrous cycle. The application of resistant nonlinear smoothing functions across raw progesterone profiles resulted in a high degree of agreement between the raw data and smoothed estimates (R(2) = 0.93). Both clustering techniques resulted in the identification of 3 similar subpopulations of progesterone profiles. The distribution of animals in these defined subpopulations and the association with cow strain and diet were examined. The early luteal phase was similar for the shape-based categories, but differed in the trait-based analyses. Differences in the late luteal phase and the onset of functional luteal regression in the different clusters were evident for both the shape- and trait-based analyses. The distribution of animals across the subpopulations identified by shape-based clustering was not associated with either cow strain or diet. However, within the 3 subpopulations identified using trait-based clustering, cow strain affected the shape of the progesterone profile. Isolation of 3 subpopulations within a small data set showed that otherwise normal progesterone profiles exhibit significant variation. The differences in the luteal phase of these subpopulations, in particular progesterone early in the luteal phase and the early onset of functional luteal regression, may provide an insight into factors associated with subfertility.
Asunto(s)
Bovinos/fisiología , Análisis por Conglomerados , Progesterona/sangre , Animales , Estro/fisiología , Femenino , Embarazo , Distribución AleatoriaRESUMEN
The objective of this study was to quantify the optimal inclusion rate of grain required to maximize nutrient digestion of a diet based on highly digestible pasture. It was hypothesized that maximum digestion would occur at a rate of grain inclusion that resulted in a culture pH of 6.0, reflecting the pH below which fiber digestion would be expected to be compromised. Four dual-flow continuous culture fermenters were used to establish the effects on digestion of replacing freeze-dried, highly digestible ryegrass with 0, 15, 30, and 45% of dry matter as 60% barley, 35% steam-flaked corn, and 5% molasses mix. The respective composite diets were fed twice daily to mimic intake patterns observed in dairy cows offered supplements during milking and offered half their daily allowance of pasture after each milking. Digesta samples were collected during the last 3 d of each of four 9-d experimental periods. Average daily culture pH decreased linearly as proportion of cereal grain in the diet increased, with average daily pH ranging from 6.29 to 5.74. Concentrations of neutral detergent fiber and total fatty acids decreased linearly with increasing proportion of cereal grain in the diet. Digestion of organic matter (OM) was maximized at an interpolated value of 24% grain inclusion and culture pH of 6.0, but the difference in the OM digestibility over the range of grain treatments from 0 to 45% was small (3 percentage units) despite pH changes over a range of 6.3 to 5.7. The relatively small change in OM digestibility was explained by reduced fiber and crude protein digestibilities being balanced by an increased digestion of nonstructural carbohydrate. Although different relationships between ruminal pH and digestibility appear to exist when cows are fed pasture alone compared with a total mixed ration, when starch supplements are included in pasture diets, the relationships associated with feeding a total mixed ration may then be more likely to apply.
Asunto(s)
Dieta/veterinaria , Digestión/fisiología , Fermentación , Hordeum/metabolismo , Lolium/metabolismo , Zea mays/metabolismo , Bacterias/metabolismo , Fibras de la Dieta/metabolismo , Proteínas en la Dieta/metabolismo , Ácidos Grasos Volátiles/análisis , Contenido Digestivo/química , Contenido Digestivo/microbiología , Concentración de Iones de Hidrógeno , Nitrógeno/análisis , Análisis de Regresión , Factores de TiempoRESUMEN
The effect of a grain-based concentrate supplement on fatty acid (FA) intake and concentration of milk FA in early lactation was investigated in grazing dairy cows that differed in their country of origin and in their estimated breeding value for milk yield. It was hypothesized that Holstein-Friesian cows of North American (NA) origin would produce milk lower in milk fat than those of New Zealand (NZ) origin, and that the difference would be associated with lower de novo synthesis of FA. In comparison, increasing the intake of concentrates should have the same effect on the FA composition of the milk from both strains. Fifty-four cows were randomly assigned in a factorial arrangement to treatments including 3 amounts of concentrate daily [0, 3, and 6 kg of dry matter (DM)/cow] and the 2 strains. The barley/steam-flaked corn concentrate contained 3.5% DM FA, with C18:2, C16:0, and C18:1 contributing 48, 18, and 16% of the total FA. The pasture consumed by the cows contained 4.6% DM FA with C18:3, C16:0, and C18:1 contributing 51, 10, and 10% of the FA, respectively. Pasture DM intake decreased linearly with supplementation, but total DM intake was not different between concentrate or strain treatments, averaging 16.2 kg of DM/cow, with cows consuming 720 g of total FA/d. Cows of the NA strain had lesser concentrations of milk fat compared with NZ cows (3.58 vs. 3.95%). Milk fat from the NA cows had lesser concentrations of C6:0, C8:0, C10:0, C12:0, C14:0, and C16:0, and greater concentrations of cis-9 C18:1, C18:2, and cis-9, trans-11 C18:2, than NZ cows. These changes indicated that in milk from NA cows had a lesser concentration of de novo synthesized FA and a greater concentration of FA of dietary origin. Milk fat concentration was not affected by concentrate supplementation. Increasing concentrate intake resulted in linear increases in the concentrations of C10:0, C12:0, C14:0, and C18:2 FA in milk fat, and a linear decrease in the concentration of C4:0 FA. The combination of NA cows fed pasture alone resulted in a FA composition of milk that was potentially most beneficial from a human health perspective; however, this would need to be balanced against other aspects of the productivity of these animals.
Asunto(s)
Alimentación Animal , Bovinos/fisiología , Suplementos Dietéticos , Grasas/química , Ácidos Grasos/análisis , Lactancia/fisiología , Leche/química , Alimentación Animal/análisis , Animales , Constitución Corporal/fisiología , Cruzamiento , Industria Lechera , Femenino , Lolium/metabolismo , Leche/metabolismo , Nueva Zelanda , América del Norte , Distribución Aleatoria , Especificidad de la EspecieRESUMEN
The aim of this study was to test the feasibility of extended lactations in pastoral systems by using divergent dairy cow genotypes [New Zealand (NZ) or North American (NA) Holstein-Friesian (HF)] and levels of nutrition (0, 3, or 6 kg/d of concentrate dry matter). Mean calving date was July 28, 2003, and all cows were dried off by May 6, 2005. Of the 56 cows studied, 52 (93%) were milking at 500 d in milk (DIM) and 10 (18%) were milking at 650 DIM. Dietary treatments did not affect DIM (605 +/- 8.3; mean +/- SEM). Genotype by diet interactions were found for total yield of milk, protein, and milk solids (fat + protein), expressed per cow and as a percentage of body weight. Differences between genotypes were greatest at the highest level of supplementation. Compared with NZ HF, NA HF produced 35% more milk, 24% more milk fat, 25% more milk protein, and at drying off had 1.9 units less body condition score (1 to 10 scale). Annualized milk solids production, defined as production achieved during the 24-mo calving interval divided by 2 yr, was 79% of that produced in a normal 12-mo calving interval by NZ HF, compared with 94% for NA HF. Compared with NZ HF, NA HF had a similar 21-d submission rate (85%) to artificial insemination, a lower 42-d pregnancy rate (56 vs. 79%), and a higher final nonpregnancy rate (30 vs. 3%) when mated at 451 d after calving. These results show that productive lactations of up to 650 d are possible on a range of pasture-based diets, with the highest milk yields produced by NA HF supplemented with concentrates. Based on the genetics represented, milking cows for 2 yr consecutively, with calving and mating occurring every second year, may exploit the superior lactation persistency of high-yielding cows while improving reproductive performance.
Asunto(s)
Fenómenos Fisiológicos Nutricionales de los Animales/fisiología , Bovinos/genética , Bovinos/fisiología , Lactancia/fisiología , Poaceae , Reproducción/fisiología , Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales/genética , Animales , Peso Corporal/fisiología , Femenino , Genotipo , Estado de Salud , Lactancia/genética , Lípidos/análisis , Leche/química , Leche/metabolismo , Proteínas de la Leche/análisis , Embarazo , Índice de Embarazo , Reproducción/genética , Factores de TiempoRESUMEN
The Molly model predicts various aspects of digestion and metabolism in the cow, including nutrient partitioning between milk and body stores. It has been observed previously that the model underpredicts milk component yield responses to nutrition and consequently overpredicts body energy store responses. In Molly, mammary enzyme activity is represented as an aggregate of mammary cell numbers and activity per cell with minimal endocrine regulation. Work by others suggests that mammary cells can cycle between active and quiescent states in response to various stimuli. Simple models of milk production have demonstrated the utility of this representation when using the model to simulate variable milking and nutrient restriction. It was hypothesized that replacing the current representation of mammary cells and enzyme activity in Molly with a representation of active and quiescent cells and improving the representation of endocrine control of cell activity would improve predictions of milk component yield. The static representation of cell numbers was replaced with a representation of cell growth during gestation and early lactation periods and first-order cell death. Enzyme capacity for fat and protein synthesis was assumed to be proportional to cell numbers. Enzyme capacity for lactose synthesis was represented with the same equation form as for cell numbers. Data used for parameter estimation were collected as part of an extended lactation trial. Cows with North American or New Zealand genotypes were fed 0, 3, or 6 kg of concentrate dry matter daily during a 600-d lactation. The original model had root mean square prediction errors of 17.7, 22.3, and 19.8% for lactose, protein, and fat yield, respectively, as compared with values of 8.3, 9.4, and 11.7% for the revised model, respectively. The original model predicted body weight with an error of 19.7% vs. 5.7% for the revised model. Based on these observations, it was concluded that representing mammary synthetic capacity as a function of active cell numbers and revisions to endocrine control of cell activity was meritorious.
Asunto(s)
Bovinos/fisiología , Lactancia/fisiología , Glándulas Mamarias Animales/citología , Glándulas Mamarias Animales/enzimología , Modelos Biológicos , Animales , Constitución Corporal/fisiología , Peso Corporal/fisiología , Simulación por Computador , Industria Lechera , Grasas/análisis , Femenino , Genotipo , Lactosa/análisis , Leche/química , Proteínas de la Leche/análisis , Factores de TiempoRESUMEN
Data from 113 lactations across 76 cows between the years 2002 to 2004 were used to determine the effect of strain of Holstein-Friesian (HF) dairy cow and concentrate supplementation on milk production, body weight (BW), and body condition score (BCS; 1 to 5 scale) lactation profiles. New Zealand (NZ) and North American (NA) HF cows were randomly allocated to 1 of 3 levels of concentrate supplementation [0, 3, or 6 kg of dry matter (DM)/cow per d] on a basal pasture diet. The Wilmink exponential model was fitted within lactation (Y(DIM) = a + b e(-0.05 x DIM) + c x DIM). The median variation explained by the function for milk yield was 86%, between 62 and 69% for milk composition, and 80 and 70% for BW and BCS, respectively. North American cows and cows supplemented with concentrates had greater peak and 270-d milk yield. Concentrate supplementation tended to accelerate the rate of incline to peak milk yield, but persistency of lactation was not affected by either strain of HF or concentrate supplementation. No significant strain by diet interaction was found for parameters reported. New Zealand cows reached nadir BCS 14 d earlier and lost less BW (22 kg) postcalving than NA cows. Concentrate supplementation reduced the postpartum interval to nadir BW and BCS, and incrementally increased nadir BCS. New Zealand cows gained significantly more BCS (i.e., 0.9 x 10(-3) units/d more) postnadir than NA cows, and the rate of BCS replenishment increased linearly with concentrate supplementation from 0.5 x 10(-3) at 0 kg of DM/d to 0.8 x 10(-3) and 1.6 x 10(-3) units/d at 3 and 6 kg of DM/d concentrates, respectively. Although there was no significant strain by diet interaction for parameters reported, there was a tendency for a strain by diet interaction in 270-d BCS, suggesting that the effect of concentrate supplementation on BCS gain was, at least partly, strain dependent.
Asunto(s)
Bovinos/fisiología , Dieta/veterinaria , Suplementos Dietéticos , Lactancia/fisiología , Animales , Composición Corporal , Peso Corporal , Cruzamiento , Bovinos/genética , Industria Lechera/métodos , Femenino , Genotipo , Lípidos/análisis , Leche/química , Proteínas de la Leche/análisis , Distribución Aleatoria , Factores de TiempoRESUMEN
Fifty-two multiparous dairy cows were allocated to 4 treatments consuming 5.4, 8.2, 10.0, or 11.0 kg/d of pasture dry matter per cow for 27 +/- 9.6 d precalving. This equated to 1.3, 1.9, 2.4, and 2.6% of body weight (BW; not including the conceptus weight). Following calving, all cows were fed ad libitum on pasture. Blood was sampled 17 d precalving, on day of calving, and on d 1, 2, 3, 4, 7, 14, 28, and 35 postcalving. Results suggest that the near-term grazing dairy cow requires 1.05 MJ of ME/kg of BW(0.75) and that previous estimates of energy requirements were underestimated. Precalving plasma concentrations of glucose, insulin-like growth factor-1, and leptin increased quadratically with increasing pasture intake. This was associated with precalving plasma concentrations of growth hormone that declined linearly, and concentrations of nonesterified fatty acids and beta-hydroxybutyrate that declined quadratically with increasing dry matter intake (DMI). Postcalving plasma concentrations of these metabolites showed no lasting effect of precalving feeding. The effect of precalving nutrition on milk production was small, and other than milk fat, was confined to wk 1 postcalving. Milk fat yield increased with increasing precalving DMI and calving body condition score until wk 3 post-calving, after which treatment effects were not evident. These results indicate that the level of feeding in grazing dairy cows during the last month before calving has only small effects on cow metabolic and hormonal status, and on milk production in the first 5 wk of lactation.
Asunto(s)
Alimentación Animal , Bovinos/metabolismo , Hormonas/sangre , Lactancia/fisiología , Parto , Ácido 3-Hidroxibutírico/sangre , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Glucemia/análisis , Composición Corporal , Peso Corporal , Calcio/sangre , Ácidos Grasos no Esterificados/sangre , Femenino , Hormona del Crecimiento/sangre , Factor I del Crecimiento Similar a la Insulina/análisis , Leptina/sangre , Lípidos/análisis , Magnesio/sangre , Leche/química , Periodo Posparto , Embarazo , Atención PrenatalRESUMEN
Dairy cows grazing high-digestibility pastures exhibit pronounced diurnal variation in ruminal pH, with pH being below values considered optimal for digestion. Using a dual-flow continuous culture system, the hypothesis that minimizing diurnal variation in pH would improve digestion of pasture when pH was low, but not at a higher pH, was tested. Four treatments were imposed, with pH either allowed to exhibit normal diurnal variation around an average pH of 6.1 or 5.6, or maintained at constant pH. Digesta samples were collected during the last 3 d of each of four, 9-d experimental periods. A constant pH at 5.6 compared with a constant pH of 6.1 reduced the digestibility of organic matter (OM), neutral detergent (NDF), and acid detergent fiber (ADF) by 7, 14, and 21%, respectively. When pH was allowed to vary (averaging 5.6), digestion of OM, NDF, and ADF were reduced by 15,30, and 36%, respectively, compared with pH varying at 6.1. There was little difference in digestion parameters when pH was either constant or varied with an average pH of 6.1. However, when average pH was 5.6, maintaining a constant pH significantly increased digestion of OM, NDF, and ADF by 5, 25, and 24% compared with a pH that exhibited normal diurnal variation. These in vitro results show that gains in digestibility and potential milk production can be made by minimizing diurnal variation in ruminal pH, but only when ruminal pH is low (5.6). However, larger gains in productivity can be achieved by increasing average daily ruminal pH from 5.6 to 6.1.
Asunto(s)
Bovinos/fisiología , Ritmo Circadiano/fisiología , Fibras de la Dieta/metabolismo , Digestión , Lolium , Rumen/química , Alimentación Animal , Animales , Reactores Biológicos , Ácidos Grasos Volátiles/análisis , Femenino , Fermentación , Concentración de Iones de Hidrógeno , Modelos Biológicos , Rumen/metabolismoRESUMEN
The impact of nutrition on lactation can be separated into acute effects, affecting day-to-day yield, and chronic effects, which govern the persistency of lactation and rate of decline of the lactation curve. A mathematical model of the mammary gland was constructed to investigate both acute and chronic effects. Mammary growth is expressed in terms of the dynamics of populations of active (secreting) and quiescent (engorged) alveoli. The secretion rate of active alveoli is expressed in terms of the energy status of the dam. The model was fitted to data from a 2 x 2 factorial trial in which lactation curves were measured for heifers of two different genotypes (North American and New Zealand Holstein-Friesians) fed two different diets [grass and total mixed rations (TMR)]. Total formation of alveoli during pregnancy and lactation was statistically the same across all groups despite differences between diets, in the rate of formation of alveoli at parturition. The senescence rate of alveoli was significantly higher for heifers fed grass compared with heifers fed TMR, which corresponds to better persistency for heifers fed TMR. Heifers fed TMR had a higher rate of reactivation of quiescent alveoli than heifers fed grass, which also contributes to increased persistence for heifers fed TMR. There was a genotype x diet interaction in the rate of quiescence of active alveoli: the North American-Grass group had a higher rate of quiescence than the other three groups, perhaps reflecting differences in selection pressures between the New Zealand and North American genotypes.
Asunto(s)
Fenómenos Fisiológicos Nutricionales de los Animales , Bovinos/fisiología , Metabolismo Energético , Lactancia , Glándulas Mamarias Animales/crecimiento & desarrollo , Modelos Biológicos , Animales , Dieta , Genotipo , Lactancia/genética , Glándulas Mamarias Animales/fisiología , Matemática , Leche/química , Nueva Zelanda , América del Norte , PoaceaeRESUMEN
A continuous culture system was used to investigate ruminal digestion in response to increased pasture intake and three different substitution rates (SR) in a 4 x 4 Latin square design. The treatments were 1) low pasture (55 g dry matter (DM)/d, 2) medium pasture (MP, 65 g DM/d), 3) high pasture (75 g DM/d), and 4) pasture (45 g DM/d) plus concentrate (PC, 30 g DM/d). Treatments were designed to produce a low (0.33), medium (0.67), and high (1.00) SR (g of pasture/g of concentrate) by contrasting the low, medium, and high pasture intake treatments with the pasture plus concentrate treatment, respectively. Pasture was fed at 0630, 1000, 1730, and 2100 h, and concentrate at 0600 and 1700 h. Digestibility of DM and neutral detergent fiber were not affected by the amount of pasture. As the amount of pasture increased, pH decreased linearly, and total volatile fatty acid and NH3-N concentrations, and nonammonia N and bacterial N flows increased linearly. Concentrate supplementation did not affect DM digestibility at high SR but increased DM digestibility at low SR. Concentrate supplementation reduced pH and NH3-N concentrations at the three SR. Concentrate supplementation reduced the ratio of rumen degradable N to rumen degradable organic matter; however, the mechanism depended on the SR. High SR, concentrate supplementation reduced rumen degradable N, which reduced NIH-N concentration without affecting bacterial N flow. At low SR, concentrate supplementation increased rumen degradable organic matter, which reduced NH3-N concentration and increased bacterial N flow. Based on these results, at low SR, concentrate supplementation may enhance animal performance because of higher total DM intake and synthesis of microbial protein.
Asunto(s)
Bovinos/fisiología , Dieta , Digestión , Fermentación , Nitrógeno/metabolismo , Rumen/metabolismo , Amoníaco/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Bacterias/metabolismo , Fibras de la Dieta/metabolismo , Proteínas en la Dieta/administración & dosificación , Ingestión de Alimentos , Ingestión de Energía , Ácidos Grasos Volátiles/análisis , Femenino , Concentración de Iones de Hidrógeno , Nitrógeno/análisisRESUMEN
Literature with data from dairy cows on pasture was reviewed to evaluate the effects of supplementation on intake, milk production and composition, and ruminal and postruminal digestion. Low dry matter intake (DMI) of pasture has been identified as a major factor limiting milk production by high producing dairy cows. Pasture DMI in grazing cows is a function of grazing time, biting rate, and bite mass. Concentrate supplementation did not affect biting rate (58 bites/min) or bite mass (0.47 g of DM/bite) but reduced grazing time 12 min/d per kilogram of concentrate compared with unsupplemented cows (574 min/d). Substitution rate, or the reduction in pasture DMI per kilogram of concentrate, is a factor which may explain the variation in milk response to supplementation. A negative relationship exists between substitution rate and milk response; the lower the substitution rate the higher the milk response to supplements. Milk production increases linearly as the amount of concentrate increases from 1.2 to 10 kg DM/d, with an overall milk response of 1 kg milk/kg concentrate. Compared with pasture-only diets, increasing the amount of concentrate supplementation up to 10 kg DM/d increased total DMI 24%, milk production 22%, and milk protein percentage 4%, but reduced milk fat percentage 6%. Compared with dry ground corn, supplementation with nonforage fiber sources or processed corn did not affect total DMI, milk production, or milk composition. Replacing ruminal degradable protein sources with ruminal undegradable protein sources in concentrates did not consistently affect milk production or composition. Forage supplementation did not affect production when substitution rate was high. Fat supplementation increased milk production by 6%, without affecting milk fat and protein content. Increasing concentrate from 1.1 to 10 kg DM/d reduced ruminal pH 0.08 and NH3-N concentration 6.59 mg/dl, compared with pasture-only diets. Replacing dry corn by high moisture corn, steam-flaked or steam-rolled corn, barley, or fiber-based concentrates reduced ruminal NH3-N concentration 4.36 mg/dl. Supplementation did not affect in situ pasture digestion, except for a reduction in rate of degradation when high amounts of concentrate were supplemented. Supplementation with energy concentrates reduced digestibility of neutral detergent fiber and intake of N but did not affect digestibility of organic matter or flow of microbial N.
Asunto(s)
Bovinos/fisiología , Digestión , Lactancia/fisiología , Leche/metabolismo , Rumen/metabolismo , Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Fibras de la Dieta/administración & dosificación , Fibras de la Dieta/metabolismo , Suplementos Dietéticos , Ingestión de Alimentos , Ingestión de Energía/fisiología , Grasas/análisis , Femenino , Concentración de Iones de Hidrógeno , Leche/química , Proteínas de la Leche/análisis , Nitrógeno/metabolismo , Poaceae , Rumen/químicaRESUMEN
This study identified suitable predictors of ruminal pH and identified relationships between ruminal pH and animal measures for diets based on fresh pasture. Animal and dietary variables (121 treatment means from six countries) were collated from 23 studies of lactating dairy cows fed pasture. Mean daily ruminal pH ranged from 5.6 to 6.7 across studies. Within studies, a low ruminal pH was associated with higher (P < 0.05; r2 > 0.40) microbial N flow from the rumen, total and individual volatile fatty acid concentrations, milk and milk component yields, and dry matter intake, and with lower (P < 0.05; r2 > 0.30) concentrations of milk fat, fat:protein, and acetate:propionate. Large variation between studies meant that these ruminal and production variables could not be used to make reliable predictions of ruminal pH in future pasture-based studies or feeding scenarios. Ruminal pH was positively related (P < 0.05; r2 < 0.15) to forage neutral detergent fiber (NDF) and NDF content within study, and negatively related (P = 0.001; r2 = 0.14) to nonstructural carbohydrate across studies. No single dietary variable, or group of variables, could be used to make a reliable prediction of ruminal pH. Estimates of effective fiber for diets containing only pasture were made using the Cornell Net Carbohydrate and Protein System ruminal pH equation. Mean effectiveness of fiber in pasture was 43% of NDF, and ranged from 17 to 78% across studies. High flows of microbial nitrogen, milk, milk fat yield, and dry matter intake suggested that the performance of cows fed high quality pasture was not limited when mean ruminal pH decreased to 5.8.
Asunto(s)
Bovinos/metabolismo , Dieta , Rumen/química , Acetatos/análisis , Animales , Butiratos/análisis , Fibras de la Dieta/administración & dosificación , Digestión , Ácidos Grasos Volátiles/análisis , Femenino , Concentración de Iones de Hidrógeno , Lactancia , Lípidos/análisis , Leche/química , Proteínas de la Leche/análisis , Propionatos/análisisRESUMEN
The increased risk of periparturient hypocalcaemia through offering high-K feeds in the precalving period has been reported previously. Two experiments (experiment 1 and 2) investigated the effect of KCl fertilizer on pasture mineral concentration, the dietary cation-anion difference (DCAD), and the subsequent effect of this on periparturient plasma and urine mineral status. Experiment 2 examined the effect of precalving Mg source (MgO, MgSO4, and MgCl2) and postcalving Ca supplementation on the concentration of Ca and Mg in plasma and urine. Unexpectedly, pasture DCAD increased (P = 0.06) from 434 to 535 mEq/kg DM in experiment 1 as pasture K concentration decreased from 4.2 to 3.5%, primarily because of a corresponding and greater decrease in pasture Cl concentration (from 1.9 to 1.3%). Plasma Ca or Mg concentrations were not affected by pasture K concentration. A linear decline (P < 0.10) in urine Mg suggested a decline in Mg absorption as pasture K increased. In experiment 2, pasture DCAD decreased (P < 0.05) linearly from 403 to 350 mEq/kg DM as pasture K concentration decreased from 3.8 to 3.3%. However, precalving urine pH was not affected by the declining DCAD. Postcalving plasma Ca concentration was affected by precalving Mg source with MgSO4 > MgCl2 > MgO. Differences in acid-base balance do not explain the difference between Mg salts. These results indicate that precalving dietary S and Cl concentration plays an important role in Ca homeostasis, in addition to its role in acid-base balance. Supplementation with Ca postcalving increased plasma Ca concentration for 2 d postcalving. Milk production was not affected.
Asunto(s)
Calcio/sangre , Bovinos/fisiología , Cloro/administración & dosificación , Dieta , Homeostasis , Azufre/administración & dosificación , Animales , Aniones , Carbonato de Calcio/administración & dosificación , Cationes , Suplementos Dietéticos , Femenino , Fertilizantes , Concentración de Iones de Hidrógeno , Trabajo de Parto , Magnesio/sangre , Cloruro de Magnesio/administración & dosificación , Sulfato de Magnesio/administración & dosificación , Potasio/análisis , Cloruro de Potasio/administración & dosificación , Embarazo , OrinaRESUMEN
Many models of digestion assume steady-state conditions and do not account for diurnal variation in the rumen environment. This experiment examined the relationships between diurnal pH, pasture digestion, and microbial protein synthesis. Four dual-flow continuous culture fermenters were used to test the effect of increasing time at suboptimal pH on parameters of digestion. Fermentation of high quality pasture was controlled at pH 5.4 (suboptimal) for four intervals during each 24-h period (0, 4, 8, and 12 h) according to a 4 x 4 Latin square design. During the remainder of each day, pH was controlled at 6.3 (optimal). Samples were collected during the last 3 d of each of the four 9-d experimental periods. A negative quadratic relationship was observed between time at suboptimal pH and apparent digestibility of organic matter and dry matter. The largest reduction in digestibility of organic matter, dry matter, and neutral detergent fiber was exhibited after 4 h at suboptimal pH. A negative linear relationship was found between time at suboptimal pH and microbial N flow, with the greatest decline in microbial N flow occurring at 12 h at suboptimal pH. These results suggest that the period of time that pH is below optimal may be more critical for digestion than the relationship between mean daily pH and optimal pH. Modeling non-steady-state ruminal conditions to account for diurnal variation in the ruminal environment may improve the prediction of digestion, especially fiber.
Asunto(s)
Bacterias/metabolismo , Proteínas Bacterianas/biosíntesis , Bovinos/metabolismo , Ritmo Circadiano , Digestión , Rumen/microbiología , Alimentación Animal , Animales , Reactores Biológicos , Fibras de la Dieta/metabolismo , Ácidos Grasos Volátiles/análisis , Fermentación , Concentración de Iones de Hidrógeno , Modelos Biológicos , Rumen/metabolismo , Factores de TiempoRESUMEN
The ruminal pH of dairy cows fed high quality pasture is often below values recommended to optimize digestion. Four continuous culture fermenters were used to determine the pH required for the optimal digestion of pasture. High quality pasture was fermented at four controlled levels of pH (5.4, 5.8, 6.2, and 6.6) according to a 4 x 4 Latin square design. Automatic infusion of 5 M NaOH and 5 M HCl controlled pH to +/- 0.1. Digesta samples were collected during the last 3 d of each of the four 9-d experimental periods. Digestion and synthesis of microbial protein were largely insensitive to pH across a broad range of pH (5.8 to 6.6), but a large reduction in both occurred when pH was 5.4. The digestibility of pasture dry matter and synthesis of microbial protein were optimized at pH 6.35 and 6.13, respectively. The proportions of individual volatile fatty acids were not changed as pH increased. Digestion of high quality pasture in continuous culture was comparable to that predicted by the Cornell Net Carbohydrate and Protein System between pH 6.2 and 6.6. However, the model underpredicted organic matter and fiber digestibility between pH 5.4 and 5.8, compared with values obtained in continuous culture. This suggests that when ruminal pH is less than 6.2, the model may over-predict the production response to supplementation of high quality pasture with an effective fiber source.
Asunto(s)
Bacterias/metabolismo , Bovinos/metabolismo , Intestinos/microbiología , Lolium/metabolismo , Alimentación Animal , Animales , Reactores Biológicos , Fibras de la Dieta/metabolismo , Digestión , Ácidos Grasos Volátiles/análisis , Fermentación , Concentración de Iones de Hidrógeno , Modelos BiológicosRESUMEN
This study evaluated the Cornell Net Carbohydrate and Protein System for dairy cows consuming diets based on pasture, assessed the sensitivity of the model to critical inputs, and demonstrated application opportunities. Data were obtained from four grazing experiments and four indoor pasture feeding experiments (25 dietary treatments) involving dairy cows in New Zealand and the US. The model provided a reasonably good estimate of changes in body condition score (r2 = 0.78; slope not significantly different from 1), estimated energy balance (r2 = 0.76; slope not significantly different from 1), blood urea N (r2 = 0.94; underprediction bias of 0.5%), microbial N flow (r2 = 0.88; slope not significantly different from 1), and milk production. The model underpredicted dry matter intake (r2 = 0.80; 13% bias) and overpredicted ruminal pH (r2 = 0.47; 1.7% bias). Predicted milk production was especially sensitive to changes in pasture lignin content, effective fiber, rate of fiber digestion, and amino acid composition of ruminal microbes. Milk production was first-limited by the supply of metabolizable energy when only high quality pasture was fed, but specific amino acids limited milk production when more than 20% of the diet consisted of a grain supplement. These results indicate that the Cornell Net Carbohydrate and Protein System can be used for dairy cows in a grazing system to make realistic predictions of performance.
Asunto(s)
Alimentación Animal , Bovinos/fisiología , Dieta , Carbohidratos de la Dieta/administración & dosificación , Proteínas en la Dieta/administración & dosificación , Lactancia/fisiología , Aminoácidos/metabolismo , Animales , Bacterias/metabolismo , Composición Corporal , Fibras de la Dieta/administración & dosificación , Fibras de la Dieta/metabolismo , Digestión , Ingestión de Alimentos , Ingestión de Energía , Femenino , Concentración de Iones de Hidrógeno , Lignina/administración & dosificación , Modelos Biológicos , Rumen/metabolismo , Rumen/microbiología , Sensibilidad y EspecificidadRESUMEN
We examined the effect of intake of fresh pasture on concentrations of conjugated linoleic acid in milk fat. Sixteen Holstein cows were paired and divided into either the control group or the grazing group. The study involved initial, transition, and final periods. During the initial period, all cows consumed a total mixed diet. Cows in the control group were fed the total mixed diet throughout the study, and cows in the grazing group were gradually adjusted to a diet consisting of intensively managed pasture. Performance of cows in the grazing group was significantly reduced from that of cows in the control group during the final period (dry matter intake, 19% less; milk yield, 29.6 vs. 44.1 kg/d; and live weight, 40 kg less). During the initial period, when both groups were consuming a total mixed diet, concentrations of conjugated linoleic acid in milk fat were similar (X = 5.1 mg/g of milk fat). As the grazing group was gradually adjusted to pasture, concentrations of conjugated linoleic acid in milk gradually increased. During the final period, when cows in the grazing group were consuming a diet consisting of pasture only, conjugated linoleic acid concentrations in the milk fat were doubled (10.9 vs. 4.6 mg/g of milk fat). Furthermore, results showed the individual consistency of the milk fat content of conjugated linoleic acid over time but also demonstrated substantial variation among individual cows within treatment groups. Overall, this study indicated that the concentration of conjugated linoleic acid in milk fat is enhanced by dietary intake of fresh pasture.