RESUMEN
Few cases have been reported in which the aspiration of a single follicle led to the recovery of two conjoined oocytes surrounded by a single zona pellucida. This report describes a successful embryo transfer with subsequent live birth derived from conjoined oocytes, and a later pair of conjoined oocytes in the same patient. After oocyte retrieval from a patient with polycystic ovary syndrome, two pairs of conjoined oocytes were collected. One oocyte was fertilized using in vitro fertilization (IVF) and developed to the blastocyst stage. This blastocyst was cryopreserved and later transferred to the uterus after separating the unfertilized conjoined oocyte. A successful pregnancy and healthy live birth was achieved. Two years later, the patient returned for a second IVF; one pair of conjoined oocytes was detected. One of the pair was fertilized and developed to a blastocyst, but was not transferred. We demonstrate that selective fertilization of a mature oocyte from conjoined oocytes by IVF can lead to the development of a blastocyst and subsequent pregnancy and live birth. To our knowledge, this is the second case report of successful live birth from conjoined oocytes. It may be the first case of repeated fertile conjoined oocytes from the same patient.
Asunto(s)
Blastocisto/fisiología , Fertilización In Vitro , Oocitos/crecimiento & desarrollo , Síndrome del Ovario Poliquístico/fisiopatología , Adulto , Criopreservación , Transferencia de Embrión , Femenino , Humanos , Nacimiento Vivo , Recuperación del Oocito/métodos , Oocitos/patología , Embarazo , Índice de Embarazo , Transferencia de un Solo Embrión , Inyecciones de Esperma Intracitoplasmáticas , Vitrificación , Zona Pelúcida/fisiologíaRESUMEN
Preliminary data from a prospective randomized study of assisted hatching (AH) of thawed human blastocysts demonstrated that the rate of complete hatching was significantly higher for AH performed near the inner cell mass (ICM) than for AH at the side opposite to the ICM. This suggests the existence of polarity in the hatching process in human blastocysts, which requires further evaluation.