RESUMEN

Antimicrobial chitosan-polyethylene oxide (CS-PEO) nanofibrous mats containing ZnO nanoparticles (NPs) and hydrocortisone-imipenem/cilastatin-loaded ZnO NPs were produced by electrospinning technique. The FE-SEM images displayed that the spherical ZnO NPs were ∼70-200nm in size and the CS-PEO nanofibers were very uniform and free of any beads which had average diameters within the range of ∼20-130nm. For all of the nanofibrous mats, the water uptakes were the highest in acidic medium but they were decreased in the buffer and the least swellings were obtained in the alkaline environment. The drug incorporated mat preserved its bactericidal activity even after it was utilized in the release experiment for 8days in the PBS buffer. The hydrocortisone release was increased to 82% within first 12h while the release rate of imipenem/cilastatin was very much slower so that 20% of the drug was released during this period of time suggesting this nanofibrous mat is very suitable to inhibit inflammation (by hydrocortisone) and infection (using imipenem/cilastatin antibiotic and ZnO NPs) principally for the wound dressing purposes.

Asunto(s)

Antibacterianos , Antiinflamatorios , Cilastatina , Sistemas de Liberación de Medicamentos , Hidrocortisona , Imipenem , Antibacterianos/administración & dosificación , Antibacterianos/química , Antiinflamatorios/administración & dosificación , Antiinflamatorios/química , Quitosano/administración & dosificación , Quitosano/química , Cilastatina/administración & dosificación , Cilastatina/química , Combinación Cilastatina e Imipenem , Preparaciones de Acción Retardada/administración & dosificación , Preparaciones de Acción Retardada/química , Combinación de Medicamentos , Liberación de Fármacos , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Hidrocortisona/administración & dosificación , Hidrocortisona/química , Imipenem/administración & dosificación , Imipenem/química , Nanofibras/administración & dosificación , Nanofibras/química , Nanopartículas/administración & dosificación , Nanopartículas/química , Polietilenglicoles/administración & dosificación , Polietilenglicoles/química , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo , Óxido de Zinc/administración & dosificación , Óxido de Zinc/química

RESUMEN

Antimicrobial chitosan-polyethylene oxide (CS-PEO) nanofiber mats loaded with 3, 5 and 10% (w/w) of zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs, ∼60nm diameter) were developed by electrospinning technique. The CS-PEO-GA-3% ZIF-8 NPs crosslinked with glutaraldehyde (GA) vapor was also prepared. The electrospun mats were characterized by various analysis including FE-SEM, EDAX, elemental mapping, FT-IR, contact angle, TGA/DSC as well as tensile strength analysis. The nanofibers had average diameters within the range ∼70-120nm. Antimicrobial activities of the CS-PEO and CS-PEO-3% ZIF-8 mats were evaluated by the viable cell-counting method for determining their effectiveness in reducing or halting the growth of Staphylococcus aureus and Escherichia coli bacteria so that the CS-PEO mat containing 3% ZIF-8 revealed 100% bactericidal activity against both kinds of bacteria. The crosslinked CS-PEO-GA-3% ZIF-8 NPs sample was less thermally stable but more hydrophilic than its related non-crosslinked mat reflecting there was no need to crosslink the fibers using a chemical crosslinker having adverse effects. The highest hydrophobicity and appropriate thermal and tensile properties of CS-PEO-3% ZIF-8 NPs among those of the mats including 5 and 10% ZIF-8 NPs suggested that the mentioned mat is the most suitable sample for food coating applications.

Asunto(s)

Quitosano/química , Escherichia coli/crecimiento & desarrollo , Membranas Artificiales , Nanocompuestos/química , Polietilenglicoles/química , Staphylococcus aureus/crecimiento & desarrollo , Nanocompuestos/ultraestructura

RESUMEN

The antimicrobial chitosan-polyethylene oxide (CS-PEO) nanofibrous mats were developed by electrospinning technique for wound dressing applications. Indeed, a green route was introduced for fabrication of antibacterial mats loaded with 0.25% and 0.50% (w/w) of bioactive silver nanoparticles (Ag NPs, ∼70nm diameter) reduced by Falcaria vulgaris herbal extract. The mats were characterized by FE-SEM, EDAX, elemental mapping, FT-IR, contact angle, TGA/DSC as well as tensile strength analysis. All of the nanofibers had an average ∼200nm diameter. Interestingly, both of the CS-PEO mats containing 0.25% and 0.50% bioactive F. vulgaris-Ag NPs revealed 100% bactericidal activities against both Staphylococcus aureus and Escherichia coli bacteria. The silver release from nanofiber mats was sharply increased within first eight hours for both CS-PEO mats including 0.25% and 0.50% F. vulgaris-Ag NPs but after that the Ag nanoparticles were released very slowly (almost constant). The improved hydrophilicity, higher tensile strength and much greater silver release for CS-PEO-0.50% F. vulgaris-Ag NPs relative to those of the CS-PEO 0.25% F. vulgaris-Ag NPs suggested that the former was superior for biomedical applications.

Asunto(s)

Quitosano/química , Electricidad , Nanopartículas del Metal/química , Nanocompuestos/química , Nanotecnología , Polietilenglicoles/química , Plata/química , Antibacterianos/química , Antibacterianos/farmacología , Escherichia coli/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacos , Resistencia a la Tracción , Agua/química