RESUMEN
We studied the levels of acute inflammation phase proteins (AIPP), production of alpha, gamma and serum interferons, the activity of epsilon-receptors of T-lymphocytes in 39 patients with bronchial asthma (BA). Correlations were made with the process severity and basic therapy. We discovered imbalance of immunoregulatory mechanisms: enhanced AIPP synthesis, low production of induced alpha and gamma interferons, elevated concentrations of poor-differentiated T-lymphocytes. This confirms persisting inflammation in BA.
Asunto(s)
Asma/inmunología , Asma/metabolismo , Inmunoglobulinas/inmunología , Interferones/sangre , Linfocitos T/inmunología , Proteínas Bacterianas/metabolismo , HumanosAsunto(s)
Proteínas Sanguíneas/inmunología , Interferones/inmunología , Centrales Eléctricas , Liberación de Radiactividad Peligrosa , Proteínas de Fase Aguda/inmunología , Proteínas de Fase Aguda/metabolismo , Adulto , Proteínas Sanguíneas/metabolismo , Relación Dosis-Respuesta en la Radiación , Humanos , Interferones/biosíntesis , Interferones/sangre , Leucocitos/inmunología , Leucocitos/metabolismo , Leucocitos/efectos de la radiación , Persona de Mediana Edad , Exposición Profesional , UcraniaRESUMEN
The immunomodulating effect of Newcastle disease virus (NDV) was investigated in vitro and in vivo in mice. NDV was shown to induce a mitogenic effect in splenocytes in vitro. Combined injections of NDV and CP resulted in nonspecific suppression of immunoreactivity in mice. The antibody production and development of delayed type hypersensitivity to sheep erythrocytes were markedly reduced. Injections of NDV alone slightly increased the reactions. The NDV + CP injections led also to a reduction of immune response to thymus-independent antigen, LPS. Thus, the combined injections of NDV and CP led to nonspecific suppression of T and B cell immunity in mice. The mechanisms of this form of anergy require further study.
Asunto(s)
Ciclofosfamida/farmacología , Tolerancia Inmunológica/inmunología , Virus de la Enfermedad de Newcastle/inmunología , Animales , Células Productoras de Anticuerpos/efectos de los fármacos , Células Productoras de Anticuerpos/inmunología , Células Cultivadas/efectos de los fármacos , Células Cultivadas/inmunología , Eritrocitos/inmunología , Hipersensibilidad Tardía/inmunología , Tolerancia Inmunológica/efectos de los fármacos , Inmunidad Innata/efectos de los fármacos , Inmunidad Innata/inmunología , Lipopolisacáridos/farmacología , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Serratia marcescens , Bazo/efectos de los fármacos , Bazo/inmunologíaRESUMEN
The cells responsible for production of human alpha and gamma interferons with proper inducers were studied using methods for fractionation of human blood mononuclear cells. The results indicated cooperation and mutual regulation by cells of interferon production. Monocytes were shown to play the leading role in the synthesis of acid-stable alpha interferon, whereas accessory cells were required for gamma interferon synthesis by T lymphocytes. Acid-labile alpha interferon was produced predominantly by B cells with accessory participation of T lymphocytes.
Asunto(s)
Interferón Tipo I/biosíntesis , Interferón gamma/biosíntesis , Leucocitos Mononucleares/inmunología , Linfocitos B/efectos de los fármacos , Linfocitos B/inmunología , Células Cultivadas/efectos de los fármacos , Células Cultivadas/inmunología , Enterotoxinas/farmacología , Humanos , Inductores de Interferón/farmacología , Interferón Tipo I/análisis , Interferón gamma/análisis , Leucocitos Mononucleares/efectos de los fármacos , Linfocitos/efectos de los fármacos , Linfocitos/inmunología , Monocitos/efectos de los fármacos , Monocitos/inmunología , Virus de la Enfermedad de Newcastle , Orthomyxoviridae , Fitohemaglutininas/farmacología , Staphylococcus aureus , Linfocitos T/efectos de los fármacos , Linfocitos T/inmunologíaRESUMEN
The results of the study confirm earlier reports on the stimulating effect of calcium ions on production of interferon induced by poly(I) X poly(C) and on translation of messenger RNA for interferon. Neutralization of the inhibiting activity of interferon production suppressor present in the cytoplasm of normal cells has first been demonstrated which broadens our concepts on the mechanism of action of calcium ions.
Asunto(s)
Cloruro de Calcio/farmacología , Interferones/biosíntesis , Animales , Citosol/efectos de los fármacos , Citosol/inmunología , DEAE Dextrano/farmacología , Interferones/antagonistas & inhibidores , Células L/efectos de los fármacos , Células L/inmunología , Ratones , Poli I-C/farmacología , Estimulación Química , Factores de TiempoRESUMEN
Two kinds of RNA capable of producing interferon in a heterologous cell system occur in the cells producing interferon in response to induction by Newcastle disease virus. One of them has messenger activity for interferon and is presented by a single-stranded structure with sedimentation constant of 10-22 S. This RNA appears to belong to cellular messenger RNA. The other is capable of inducing interferon production by heterogenous cells, resistant to treatment with RN-ase and has sedimentation constant greater than 35 S. In our opinion, this RNA is the primary replicative RNA of the virus-inducer.