RESUMEN
EDS alkylating agent has been shown to selectively and temporarily kill LCs in adult rats. The first newly formed single LCs appeared at 14th day post ESD and showed detectable activity for 3beta-HSD and NADH2-diaphorase, which became progressively stronger with time after treatment The ultrastructural study revealed that the progenitor LCs differentiated into immature LCs within a week, and two weeks later they were transformed into mature LCs. Therefore, the restoration of new LC population after EDS treatment repeated the dynamics of normal LC development within a similar time range. The dynamics of enzyme activity correlated with structural differentiation of the new LC population.
Asunto(s)
Diferenciación Celular/efectos de los fármacos , Células Intersticiales del Testículo/efectos de los fármacos , Células Intersticiales del Testículo/enzimología , Mesilatos/farmacología , 3-Hidroxiesteroide Deshidrogenasas/efectos de los fármacos , 3-Hidroxiesteroide Deshidrogenasas/metabolismo , Animales , Dihidrolipoamida Deshidrogenasa/efectos de los fármacos , Dihidrolipoamida Deshidrogenasa/metabolismo , Células Intersticiales del Testículo/patología , Masculino , Ratas , Ratas Wistar , Esteroides/biosíntesisRESUMEN
OBJECTIVE: To investigate degeneration and restoration patterns of spermatogenesis in relation to the changes in Leydig cells (LCs) after treatment with ethane dimethanesulfonate (EDS). MATERIALS AND METHODS: Adult Wistar male rats were treated with EDS at a dose 75 mg/kg body weight and the testes were sampled at 7, 14, 21, 35 and 49 days after treatment for histological and ultrastructural studies. RESULTS: During the first two weeks after treatment stage dependent loss of germ cells was found within seminiferous tubules that led to a profound disturbance of spermatogenesis. The restoration of seminiferous epithelium followed also in stage specific manner and in relation to development of a new LC population (third week). The development of new LCs after EDS treatment repeats the normal dynamics of postnatal LC development within a similar time range. CONCLUSION: EDS treatment of rats causes a temporary germ cell degeneration in the testis. The kinetics of disappearance of germ cells and their regeneration broadly follows the changes in LC population.