RESUMEN
Immunohistochemical study of B23/nucleophosmine (NPM), the key argyrophilic nonribosomal protein associated with the nucleolus-producing regions, in smooth muscle formations of the body of the uterus and myometrium was carried out. The uterus index, number of positively stained nucleoli in the nucleus, integral optical density, and area of positively stained nucleoli were studied by computer-aided image analysis. The results indicated significant differences in the integral optical density and less so in the areas of NPM+ nucleoli in leiomyosarcomas, but not in intact myometrium and leiomyomas. Coefficients of variations in the integral optical density and NPM area proved to be significant parameters for differentiating leiomyosarcomas by size (≤7 cm or larger), evaluating the malignancy grade and stage of the process. NPM parameters in leiomyomas and leiomyosarcomas differed significantly, depending on the levels of Ki-67 and p53 expression and the receptor status of leiomyosarcoma. The index of area and the integral optical density of NPM could serve a accessory objective differential diagnostic criteria in cellular and mitotically active liomyomas and well-differentiated leiomyosarcoma of the corpus uteri.
Asunto(s)
Proteínas Nucleares/metabolismo , Tumor de Músculo Liso/metabolismo , Neoplasias Uterinas/metabolismo , Femenino , Humanos , Inmunohistoquímica , Leiomioma/metabolismo , Leiomiosarcoma/metabolismo , Persona de Mediana Edad , Miometrio/metabolismo , NucleofosminaRESUMEN
OBJECTIVE: to study HER2 protein and HER2 gene, their heterogeneity in non-small cell lung cancer. MATERIAL AND METHODS: 218 intraoperative non-small cell lung samples were examined using tissue matrix methods. HER2 protein was determined by immunohistochemistry (clone 4B5, <
Asunto(s)
Adenocarcinoma/genética , Carcinoma de Pulmón de Células no Pequeñas/genética , Carcinoma de Células Escamosas/genética , Genes erbB-2 , Neoplasias Pulmonares/genética , Receptor ErbB-2/biosíntesis , Adenocarcinoma/epidemiología , Adenocarcinoma/metabolismo , Adenocarcinoma/patología , Carcinoma de Pulmón de Células no Pequeñas/epidemiología , Carcinoma de Pulmón de Células no Pequeñas/metabolismo , Carcinoma de Pulmón de Células no Pequeñas/patología , Carcinoma de Células Escamosas/epidemiología , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patología , Humanos , Inmunohistoquímica , Hibridación in Situ , Neoplasias Pulmonares/epidemiología , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patología , Prevalencia , Receptor ErbB-2/genéticaRESUMEN
The objective of this study was to examine cell proliferation, apoptosis and angiogenesis in pathologically unchanged lung and in non-small cell lung cancer with the use of appropriate markers. The material studied included samples of pathologically unchanged lung (n=80) and those obtained at operations in 237 cases of non-small cell lung cancer. Immunohistochemical methods were used to demonstrate Ki-67, topoisomerase IIalpha (TopoIIalpha), p53, bcl-2, bax, CD34 and podoplanin. Argyrophilic proteins associated with nucleolar organizer regions (Ag-NOR proteins), were detected by impregnation with silver nitrate. The quantitative data were obtained and the peculiarities of the expression of markers associated with proliferation, apoptosis and angiogenesis in pathologically unchanged lung were determined. In the alveoli, the labeling index of Ki-67 and Topolla was less than 1%, AgNOR area index was equal to 1.31 +/- 0.20; p53, bcl-2, bax expression was absent, density of blood vessels was equal to 86 (73-102), while lymphatic vessels were absent. In the bronchus, the labeling index of Ki-67 and TopoIIalpha were respectively 4 (1-8) and less than 1%, AgNOR area index--1.85 +/- 0.24, bax expression--100%, density of blood and lymph vessels--22 (17-31) and 4 (2-7) respectively; p53 and bcl-2 expression were absent. The results were compared with the expression of markers in non-small cell lung cancer. This comparison has fundamental and differential diagnostic value in the study of histopathological lung material. The expression of markers associated with proliferation, apoptosis and angiogenesis changes from pathologically unchanged lung to non-small cell lung cancer.
Asunto(s)
Apoptosis , Carcinoma de Pulmón de Células no Pequeñas/metabolismo , Proliferación Celular , Neoplasias Pulmonares/metabolismo , Pulmón/metabolismo , Neovascularización Patológica/metabolismo , Carcinoma de Pulmón de Células no Pequeñas/irrigación sanguínea , Carcinoma de Pulmón de Células no Pequeñas/patología , Femenino , Humanos , Pulmón/patología , Neoplasias Pulmonares/irrigación sanguínea , Neoplasias Pulmonares/patología , Masculino , Neovascularización Patológica/patologíaRESUMEN
Ninety-four lung adenocarcinoma samples obtained during surgeries were examined using a combination of immunohistochemical staining for Ki-67 antigen and silver nitrate staining for argyrophilic nucleolar organizer (Ag-NOR) proteins. In lung adenocarcinoma, we studied the correlation between the area of Ag-NOR proteins in Ki-67(+) cells and clinical and morphological TNM parameters: maximum tumor diameter (T) and stage of the disease and tumor differentiation degree (N). Survival of patients with small area of Ag-NOR proteins in Ki-67(+) cells was higher that in patients with great area of these proteins. The area of Ag-NOR proteins in Ki-67(+) cells is an independent prognostic factor in lung adenocarcinoma. The area of Ag-NOR proteins in Ki-67(+) cells correlates with clinical and morphological TNM parameters and survival of patients with lung adenocarcinoma.
Asunto(s)
Adenocarcinoma/metabolismo , Antígenos Nucleares/metabolismo , Biomarcadores de Tumor/metabolismo , Neoplasias Pulmonares/metabolismo , Región Organizadora del Nucléolo/metabolismo , Adenocarcinoma/mortalidad , Adenocarcinoma/patología , Adenocarcinoma del Pulmón , Adulto , Anciano , Femenino , Humanos , Estimación de Kaplan-Meier , Antígeno Ki-67/metabolismo , Neoplasias Pulmonares/mortalidad , Neoplasias Pulmonares/patología , Masculino , Persona de Mediana Edad , Modelos de Riesgos ProporcionalesRESUMEN
A total of 218 operation specimens from patients with nonsmall cell lung cancer were examined. The expression of HER-2 protein was studied by the immunohistochemical method, amplification of HER2 gene and centromere part of chromosome 17 (CEP17) - by the in situ chromogenic hybridization method. Moderate correlation (r=0.38, p<0.001) between the immunohistochemical status and HER2 gene amplification was detected. A relationship between TNM clinical morphological parameters and HER-2 protein content and chromosome 17 polysomia (for N parameter), HER-2 protein content, and HER2 gene amplification (for tumor histogenesis) was detected. The content of HER-2 protein and amplification of HER2 and CEP17 correlated with the clinical morphological parameters of patients with nonsmall cell lung cancer.
Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas/metabolismo , Carcinoma de Pulmón de Células no Pequeñas/patología , Cromosomas Humanos Par 17/genética , Amplificación de Genes/genética , Receptor ErbB-2/genética , Receptor ErbB-2/metabolismo , Adulto , Anciano , Femenino , Humanos , Inmunohistoquímica , Hibridación in Situ , Masculino , Persona de Mediana EdadRESUMEN
Argyrophilic proteins associated with nucleolar organizer regions (Ag-NOR proteins) and Ki-67 antigen were analyzed in 118 samples of squamous cell carcinoma of the lungs. Tumors with low and high content of Ag-NOR proteins and Ki-67 index were selected. It was found that the content of Ag-NOR proteins correlated with some clinical and morphological parameters (indexes T and N, tumor size less and more that 3 cm, stage of the disease, and tumor differentiation degree) and survival rate. High survival was associated with low content of Ag-NOR proteins and Ki-67 index and low survival correlated with high content of Ag-NOR proteins and Ki-67 index, while intermediate survival was associated with opposite values of Ag-NOR protein content and Ki-67 index. The tumor size, parameter N, and the content of Ag-NOR proteins had independent effects on patient's survival. In patients with squamous cell carcinoma of the lung without metastases in lymph nodes, survival correlated with the content of Ag-NOR proteins; in patients without metastases, survival correlated with tumor size.
Asunto(s)
Carcinoma de Células Escamosas/patología , Proliferación Celular , Neoplasias Pulmonares/patología , Proteínas de Neoplasias/metabolismo , Región Organizadora del Nucléolo/metabolismo , HumanosRESUMEN
Argyrophilic proteins associated with chromosome nucleolar organizer regions (Ag-NOR proteins) were studied in lung squamous-cell carcinoma cells. After routine staining with silver nitrate, the area index and coefficient of variations of Ag-NOR protein were estimated by computer-aided image analysis. The Ag-NOR protein area indexes of tumor cells differed in clinical morphological variants of squamous-cell cancer (differing by T and N parameters, size of the primary node <3 cm or larger, by disease stage, and differentiation degree). Ag-NOR protein variation coefficient can be used for discriminating the tumors by size (<3 cm and larger), presence/absence of metastases, and evaluation of squamous-cell cancer differentiation degree. Ag-NOR protein area index and variation coefficient can be used as additional objective differential diagnostic criteria of clinical and morphological forms of squamous-cell carcinoma of the lung.