RESUMEN
Basic 7S globulin (Bg7S), which accumulates in mature soybean (Glycine max) seeds, is an extracellular matrix protein. A large amount of Bg7S is synthesized de novo and is eluted from soybean seeds when immersed in 50-60°C water (hot water treatment, HWT). However, the Bg7S elution mechanism remains unclear. Under HWT, the seeds probably undergo heat stress and flooding stress. To obtain fundamental knowledge related to how Bg7S is eluted from hot-water-treated seeds, this study compared Bg7S elution among soybean cultivars having different flooding tolerance during pre-germination. The amounts of Bg7S eluted from seeds varied significantly among cultivars. Elution was suppressed by seed coats regarded as preventing the leakage of seed contents by rapid water imbibition. Furthermore, Bg7S expression levels differed among cultivars, although the difference did not result from any variation in Bg7S promoter sequences. However, the expression levels of Bg7S under HWT were not associated with the flooding tolerance level. Immunoelectron microscopy revealed that the Bg7S accumulated in the intercellular space of hot-water-treated seeds. Plasma membrane shrinkage was observed. The main proteins eluted from seeds under HWT were located in the extracellular space. This study clarified the mechanism of Bg7S elution from seeds under HWT.
Asunto(s)
Antígenos de Plantas/biosíntesis , Globulinas/biosíntesis , Glycine max/metabolismo , Proteínas de Almacenamiento de Semillas/biosíntesis , Semillas/metabolismo , Proteínas de Soja/biosíntesis , Secuencia de Aminoácidos , Antígenos de Plantas/genética , Secuencia de Bases , Globulinas/genética , Globulinas/metabolismo , Respuesta al Choque Térmico , Regiones Promotoras Genéticas , Transporte de Proteínas , Proteínas de Almacenamiento de Semillas/genética , Proteínas de Almacenamiento de Semillas/metabolismo , Semillas/ultraestructura , Análisis de Secuencia de Proteína , Proteínas de Soja/genética , Proteínas de Soja/metabolismo , Glycine max/ultraestructuraAsunto(s)
Lesión Renal Aguda/prevención & control , Medios de Contraste/efectos adversos , Terapia por Inhalación de Oxígeno , Lesión Renal Aguda/inducido químicamente , Anciano , Angiografía Coronaria , Creatinina/sangre , Tasa de Filtración Glomerular , Humanos , Soluciones Isotónicas , Análisis Multivariante , Oxígeno/sangre , Factores de Riesgo , Cloruro de Sodio/uso terapéuticoRESUMEN
We study the catalyst-free growth of InP nanowires using selective-area metalorganic vapor phase epitaxy (SA-MOVPE) and show that they undergo transition of crystal structures depending on the growth conditions. InP nanowires were grown on InP substrates where the mask for the template of the growth was defined. The nanowires were grown only in the opening region of the mask. It was found that uniform array of InP nanowires with hexagonal cross section and with negligible tapering were grown under two distinctive growth conditions. The nanowires grown in two different growth conditions were found to exhibit different crystal structures. It was also found that the orientation and size of hexagon were different, suggesting that the difference of the growth behavior. A model for the transition of crystal structure is presented based on the atomic arrangements and termination of InP surfaces. Photoluminescence measurement revealed that the transition took place for nanowires with diameters up to 1 microm.
Asunto(s)
Indio/química , Modelos Químicos , Modelos Moleculares , Nanoestructuras/química , Nanoestructuras/ultraestructura , Fosfinas/química , Simulación por Computador , Sustancias Macromoleculares/química , Conformación Molecular , Tamaño de la Partícula , Transición de Fase , Propiedades de SuperficieRESUMEN
Through films, we can see many aspects of a country and its times: culture, morality and religion, and views on life and death. The best films can both entertain audiences and provide viewers with opportunities to think about fundamental human problems. In this article, we use Departures (Okuribito) to examine the contemporary Japanese view of life and death. All sorts of deaths are depicted and each scene provides an insight into the contemporary Japanese view of death. We use the medium of film to consider the issue of death: what death is, the relationship that exists between life and death, and how the impurity and dignity of the dead are recognised by contemporary Japanese people. The ritual of 'encoffinment' will also be discussed, and what it suggests and reveals about Japanese views on what happens to a person when they die, and what requirements exist for someone to be able to depart from this world to the afterlife. The view of death depicted in Departures is thought to accept and even hope for a worldview that postulates continuity between life and death, wherein not only the soul but also personal individuality continues on as it existed in life. The rite of encoffinment is required to relieve the family's grief as well as to wipe away the impurity of the dead. The Japanese traditional view that the 'dead are impure' seems to die hard. It is also suggested that complicated and ambivalent attitudes towards the dead exist among contemporary Japanese people.
Asunto(s)
Cultura , Muerte , Pesar , Vida , Películas Cinematográficas , Filosofía , Actitud , Familia , Humanos , JapónRESUMEN
We report on selective-area growth of vertically aligned GaAs nanowires on Si(111) substrate. Modification of the initial Si(111) surface by pretreatment under an AsH(3) atmosphere and low-temperature growth of GaAs were important for controlling the growth orientations of the GaAs nanowire on the Si(111) surface. We also found that the size of openings strongly affected the growth morphology of GaAs nanowires on Si(111). Small diameter openings reduced the antiphase defects and improved the optical properties in the GaAs nanowires. Moreover, we realized coherent growth without misfit dislocation at the GaAs/Si interface. Finally, we demonstrated fabrication of a GaAs/AlGaAs core-shell nanowire array on a Si surface and revealed that the luminescence intensity was markedly enhanced by passivation effects. These results are promising for future III-V nanowire-based optoelectronic integration on Si platforms.
RESUMEN
Highly uniform GaAs/GaAsP coaxial nanowires were prepared via selective-area metal organic vapor phase epitaxy. Photoluminescence spectra from a single nanowire indicate that the obtained heterostructures can produce near-infrared (NIR) lasing under pulsed light excitation. The end facets of a single nanowire form a natural mirror surface to create an axial cavity, which realizes resonance and give stimulated emission. This study is a considerable advance toward the realization of nanowire-based NIR light sources.
Asunto(s)
Arsenicales/química , Cristalización/métodos , Galio/química , Láseres de Semiconductores , Rayos Láser , Nanoestructuras/química , Nanoestructuras/ultraestructura , Nanotecnología/instrumentación , Diseño Asistido por Computadora , Diseño de Equipo , Análisis de Falla de Equipo , Sustancias Macromoleculares/química , Ensayo de Materiales , Conformación Molecular , Nanotecnología/métodos , Tamaño de la Partícula , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Propiedades de SuperficieRESUMEN
Although vein graft aneurysms have been described to be atherosclerotic in nature, it has been hypothesized that vein graft aneurysms may be a part of a systemic dilating process. In the case reported here, histopathologic examination of vein graft aneurysms demonstrates aneurysmal degeneration with no atherosclerotic changes and do support the hypothesis that vein graft aneurysms may be a manifestation of a systemic dilating process.
Asunto(s)
Aneurisma/patología , Arteria Poplítea , Complicaciones Posoperatorias/patología , Vena Safena , Procedimientos Quirúrgicos Vasculares , Anciano , Dilatación Patológica , Humanos , Masculino , Vena Safena/trasplanteRESUMEN
BACKGROUND: Chronic pancreatitis and liver cirrhosis are major alcohol-related diseases in most countries. Neither their specific etiologies nor the relationship between them is fully understood. This study was designed to examine a possible association between alcoholic chronic pancreatitis (ACP) and alcoholic liver cirrhosis (ALC), and to identify factors relating to them. METHODS: The subjects were 141 consenting participants from 1087 male patients consecutively admitted to Kurihama National Hospital from July 2000 to November 2002. All were negative for major medical disorders (e.g., viral hepatitis, operative history, malignancy), except for ACP and ALC. Analysis of each subject included background information (collected by face-to-face interview, regarding quantity and duration of drinking, usual alcoholic drinks, smoking, education, employment, and marital status) and signs of ACP detected on endoscopic retrograde cholangiopancreatography (ERCP) and ALC indicated by Child-Pugh classification. Subjects consenting to genome analyses ( n = 83) were genotyped for two key alcohol-metabolizing enzymes: alcohol dehydrogenase-2 and aldehyde dehydrogenase-2. RESULTS: Grouping patients by ERCP grading and Child classification revealed a nonparallel relationship between the severities of the two diseases. This relationship held, even after controlling for several pertinent background variables (sociofamilial, drinking, clinical, and genetic factors) by logistic regression analysis. The drinking of spirits and a high daily consumption of alcohol were independent risk factors for ACP, while never-married status was the only risk factor identified with ALC among these male Japanese patients. CONCLUSIONS: Different risk factors may confer susceptibility to ACP versus ALC, which may explain the nonparallel relationship between the severities of the two diseases in Japanese alcoholics.
Asunto(s)
Aldehído Deshidrogenasa/genética , Cirrosis Hepática/epidemiología , Pancreatitis Alcohólica/epidemiología , Adulto , Alcohol Deshidrogenasa/genética , Aldehído Deshidrogenasa Mitocondrial , Pueblo Asiatico/genética , Comorbilidad , Genotipo , Humanos , Japón/epidemiología , Estilo de Vida , Cirrosis Hepática/genética , Modelos Logísticos , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Pancreatitis Alcohólica/genética , Factores de Riesgo , Estudios Seroepidemiológicos , Fumar/epidemiologíaRESUMEN
BACKGROUND: Heavy drinkers have a high incidence of chronic pancreatitis (CP), but the mechanism of alcohol-related CP is largely unknown. Exocrine pancreatic insufficiency exists in about 90% of the patients with cystic fibrosis (CF), which results from an abnormal cystic fibrosis transmembrane conductance regulator gene (CFTR). AIM: To investigate in Japanese alcoholics the association between bicarbonate concentration in pure pancreatic juice and one of the polymorphisms of the CFTR gene, the (TG)m Tn tract length in intron 8. METHODS: Fifty-six patients under treatment for alcohol dependence were stimulated by intravenous injection of secretin during endoscopic retrograde cholangiopancreatography to provide pancreatic juice specimens. Individual maximum bicarbonate concentrations (MBC) were compared with (TG)m Tn tract polymorphisms identified by directly sequencing lymphocyte DNA. RESULTS: Among the 41 patients able to provide adequate pancreatic juice specimens, 15 had low MBC and 26 had normal MBC. The frequencies of the six haplotypes identified in these patients were 17.1% (TG)11T7/(TG)llT7, 46.3% (TG)11T7/(TG)12T7, 29.3% (TG)12T7/(TG)12T7, 2.4% (TG)10T9/(TG)11T7, 2.4% (TG)12T5/(TG)11T7, and 2.4% (TG)12T6/(TG)12T7. Among the 92.7% of patients who had the common (TG) miT7/(TG)m2T7 haplotype, all of the 7 with homozygous (TG)11 alleles had normal MBC (p<0.05). CONCLUSION: Alcoholics with homozygous (TG)11 alleles in intron 8 of the CFTR gene appear to be protected against decreased MBC, compared with those who have the (TG)11/(TG)12 and (TG)12/(TG)12 genotypes, suggesting a role for CFTR gene polymorphism in the progression of alcohol-related pancreatic disease.
Asunto(s)
Bicarbonatos/análisis , Regulador de Conductancia de Transmembrana de Fibrosis Quística/genética , Jugo Pancreático/química , Pancreatitis Alcohólica/genética , Polimorfismo Genético , Adulto , Alelos , Enfermedad Crónica , Predisposición Genética a la Enfermedad , Homocigoto , Humanos , Masculino , Persona de Mediana Edad , Pancreatitis Alcohólica/metabolismo , Análisis de Secuencia de ADNRESUMEN
BACKGROUND: We have reported that the serum level of amylase, different from other pancreatic enzymes, increases temporarily after abstinence in alcoholics. To elucidate the mechanism of this phenomenon, pancreatic isoamylase, salivary isoamylase, and amylase in urine were measured together with total serum amylase. METHODS: Total serum amylase, pancreatic isoamylase, and salivary isoamylase values were measured in 38 male patients admitted to the National Alcoholism Center, Kurihama Hospital, for alcoholism after abstinence. In an investigation of amylase secretion, amylase in urine was measured in some patients after abstinence. RESULTS: In the group with abnormally high total serum amylase on admission, levels were found to decrease after abstinence. In patients with pancreatic disorders in this group, abstinence leads to a decrease in total serum amylase, but in patients with no such disorders, total serum amylase increases temporarily due to increases in salivary isoamylase. In the group with normal total serum amylase on admission, levels increased sharply after abstinence, and both pancreatic isoamylase and salivary isoamylase contributed to the gains. In the group with low total serum amylase, a sharp increase of 2-fold or more was noted after abstinence, and a major contributor was pancreatic isoamylase. The ratio of urine amylase to total serum amylase gradually declined, indicating clearly that abstinence led to a decrease in the excretion of amylase in urine. CONCLUSIONS: In cases of heavy alcohol consumption, a decrease in the production or secretion of pancreatic isoamylase and salivary isoamylase while drinking could happen. It was thus suggested that the increase in serum amylase might be due to the fact that this situation is improved by abstinence, plus the fact that excretion of amylase in urine increases during alcohol consumption, and abstinence brings about a decline in such excretion. Measurement of total serum amylase is not appropriate for diagnosing pancreatitis in alcoholic patients or those who consume large quantities of alcohol.