RESUMEN
This study was carried out to investigate the role of lead in the development of oxidative stress in the brain. We examined the rate of lipid peroxidation and we determined lipid fluorescence products (lipofuscin-like pigments - LFP) as a marker of lipid peroxidation after short in vitro incubation of rat brain homogenates with lead acetate (10(-2), 10(-4), 10(-6) M lead acetate, 2 h). Simultaneously we examined by the same method in vivo indices of oxidative stress in brains of mice exposed for 12 weeks to 0.2% lead acetate in drinking water. The results show that the concentration of LFP in rat brain homogenates increased significantly after 2 h incubation with 10(-2) M lead acetate as compared to controls (P<0.0001). This effect was not observed in lower doses of lead acetate (10(-4) and 10(-6) M). After the long-term exposure of mice to 0.2% lead acetate, pronounced accumulation of lead and significantly increased concentration of LFP (P<0.004) in the brains of exposed animals as compared to controls were observed. The evidence for the formation of specific fluorophores originating from oxidative damage was shown also in qualitative changes in 3D spectral arrays and synchronous spectra. The presented results proved the influence of lead on the activation of radical reactions in the brain after short in vitro exposure of rat brain as well as within long-term in vivo exposure in mice using lipofuscin-like pigments as an indicator of oxidative stress.
Asunto(s)
Encéfalo/efectos de los fármacos , Plomo/toxicidad , Peroxidación de Lípido/efectos de los fármacos , Lipofuscina/metabolismo , Estrés Oxidativo/efectos de los fármacos , Administración Oral , Animales , Biomarcadores/metabolismo , Encéfalo/metabolismo , Agua Potable , Radicales Libres/metabolismo , Técnicas In Vitro , Plomo/farmacocinética , Masculino , Ratones , Ratones Endogámicos , Ratas , Ratas Wistar , Espectrometría de Fluorescencia , Factores de TiempoRESUMEN
Some implications of cooperative potential of metal ions and electromagnetic fields' radiation (EMF) in carcinogenic processes are discussed. It is known that these factors, chemical and physical individually have connections with processes of oxidative stress. Special attention was paid to possible manifestation within the brain. Therefore, the entry of a few potentially neurotoxic metals into the brain is discussed.
Asunto(s)
Encéfalo/efectos de los fármacos , Encéfalo/efectos de la radiación , Campos Electromagnéticos/efectos adversos , Metabolismo Energético/efectos de los fármacos , Metabolismo Energético/efectos de la radiación , Metales Pesados/toxicidad , Animales , Encéfalo/metabolismo , Relación Dosis-Respuesta a Droga , Metales Pesados/administración & dosificación , Ratones , RatasRESUMEN
Impact of metal in vitro administration on rat tissue oxygen consumption is referred in the first part. Toxicological implications of in vivo metal administration to rats and the study of potential penetration of metal into the rat brain, which may eventually result in oxygen radical production are presented in second part.