RESUMEN

The immunological characterization of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP), and its methylenediphosphonate analogue, HMB-PCP, is described. With an EC(50) of 0.1-0.2 nM, HMB-PP is significantly more potent in stimulating human Vgamma9/Vdelta2 T cells than any other compound described so far. However, replacing the pyrophosphate by a P-CH(2)-P function abrogates the bioactivity drastically, with HMB-PCP having a EC(50) of only 5.3 microM.

Asunto(s)

Difosfatos/farmacología , Linfocitos T/efectos de los fármacos , Unión Competitiva/efectos de los fármacos , Difosfatos/química , Humanos , Técnicas In Vitro , Indicadores y Reactivos , Espectroscopía de Resonancia Magnética , Peso Molecular , Relación Estructura-Actividad