RESUMEN

The enzyme immunoassay (EIA) on nitrocellulose filters was adapted for the detection of exotoxin A in 104 P. aeruginosa strains isolated from patients and the environment in surgical wards in hospitals of Moscow and Alma-Ata. The method was shown to be highly sensitive: it permitted the detection of 5.ng of P. aeruginosa exotoxin A. The screening of 104 P. aeruginosa clinical strains by means of EIA on nitrocellulose filters revealed that these strains exotoxin A in 88.5% of cases.

Asunto(s)

Exotoxinas/análisis , Pseudomonas aeruginosa/patogenicidad , Colodión , Microbiología Ambiental , Filtración/instrumentación , Humanos , Técnicas para Inmunoenzimas/instrumentación , Kazajstán , Moscú , Pseudomonas aeruginosa/aislamiento & purificación , Servicio de Cirugía en Hospital