RESUMEN
Early defects due to the afflux of monocytic phagocytes into the inflammatory focus, rapid resorption of detritis and normalisation of correlation between contents of matrix proteinases and their inhibitors, stimulation of stromal cornea keratinocytes migration and their proliferation characterize repair of postburn defects in transplantation of the stromal equivalent. Repair against the background of stromal equivalent transplantation is of a histotypical character: collagen gel organizes and serves the basis for migration of the cornea fibroblasts and temporary matrix for growing new epithelium, while restored basal membrane is a derivative of cornea epithelium.
Asunto(s)
Implantes Absorbibles , Sustancia Propia/inmunología , Quemaduras Oculares/inmunología , Regeneración/inmunología , Animales , Diferenciación Celular , Movimiento Celular/inmunología , Colágeno/inmunología , Sustancia Propia/lesiones , Sustancia Propia/patología , Quemaduras Oculares/patología , Quemaduras Oculares/terapia , Fibroblastos/inmunología , Fibroblastos/patología , Humanos , Queratinocitos/inmunología , Queratinocitos/patología , Metaloproteinasas de la Matriz/inmunología , Fagocitos/inmunología , Fagocitos/patología , Conejos , Ratas , Ratas WistarRESUMEN
The paper contains the results of using a mew method applicable to regulation of corneal regeneration, i.e. transplantation of allogenic fibroblasts cultivated in collagen gel within an experimental model of acute alkali corneal burns in rabbits. Two experimental stages, involving 120 animals, were undertaken. The above method was used, at stage 1, on day 2 after trauma and, at stage 2, it was applied in two weeks after ocular burns with profound corneal defects were inflicted. The clinical, morphological and immune-histochemical examinations were used to show a high efficiency of the method in healing the corneal burn wounds; the mechanism of the therapeutic action of the transplant components exerted on regeneration of the affected cornea was investigated; finally, good outlooks for using the discussed method in clinical practice were proven.
Asunto(s)
Quemaduras Químicas/cirugía , Lesiones de la Cornea , Quemaduras Oculares/inducido químicamente , Quemaduras Oculares/cirugía , Fibroblastos/trasplante , Álcalis , Animales , Células Cultivadas , Colágeno , Lentes de Contacto Hidrofílicos , Geles , Humanos , Conejos , Ratas , Ratas Wistar , Factores de Tiempo , Trasplante Homólogo , Cicatrización de HeridasAsunto(s)
Biotecnología/métodos , Enfermedades de la Córnea/cirugía , Trasplante de Córnea/métodos , Biotecnología/tendencias , Células Cultivadas/trasplante , Trasplante de Córnea/tendencias , Fibroblastos/citología , Humanos , Limbo de la Córnea/citología , Trasplante de Células Madre/métodos , Trasplante de Células Madre/tendenciasRESUMEN
Sluggish wounds are characterized by impaired proportions of proinflammatory cytokines, deficiency of fibrogenic growth factors, imbalance in the system of matrix metalloproteinases and their inhibitors this preventing reparation. The study was made of biopsies obtained from patients with sluggish wounds before the treatment, 5, 10 and 15 days after transplantation on the wound of allogenic EGF-stimulated cryopreserved epidermis. The wound closure with biologically active coat was followed by the reduction of expression of proinflammatory cytokines and return to their normal correlations, higher production of fibrogenic growth factors, restoration of balance in the expression of MMP-9 and TIMP-1/TIMP-2.