RESUMEN
The effects of two gallium (Ga) compounds, Ga chloride (GaCl3) and tris(8-quinolinolato)Ga (III) on the viability of A549 human malignant lung adenocarcinoma cells were investigated. The results demonstrated that both drugs reduced the viability of A549 cells but to different extents. The inhibitory effects of tris(8-quinolinolato)Ga (III) were 10 times more profound than those produced by GaCl3. The IC50 was obtained with 2.5 microM of tris(8-quinolinolato)Ga (III) and 25 microM GaCl3 after an exposure time of 48 hours. Further, whereas the inhibitory effects of GaCl3 were both dose and time-dependent those of tris(8-quinolinolato)Ga (III) appeared to be only dose-dependent, indicating differences in their mechanism of action. Comparison with data drawn from the literature suggests that GaCl3 seems to be in the same range of activity as Ga nitrate or Ga-pyridoxal isocotinoyl hydrazone. Tris(8-quinolinolato)Ga (III) could be as effective as transferrin-Ga, but with the advantage of oral administration and a greater bioavailability of the tris(8-quinolinolato)Ga (III) compound.
Asunto(s)
Antineoplásicos/toxicidad , División Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Galio/toxicidad , Compuestos Organometálicos/toxicidad , Oxiquinolina/análogos & derivados , Adenocarcinoma , Humanos , Cinética , Neoplasias Pulmonares , Oxiquinolina/toxicidad , Células Tumorales CultivadasRESUMEN
Microorganisms are very sensitive to the effect of metal ions and a deficit or an excess of essential metal ions may induce some cell disturbances. The main objective of this research is to evaluate the effect of toxic metal ions. Doing that, the effects of metal ions on the electrophysical properties of the cell membrane, probed by electroorientational spectroscopic techniques and analysis of the electrophoretic mobility of cells have been chosen for investigation. The purpose of this work is to determine the optimal conditions of investigation of different kinds of microorganisms exposed to Cu. pH had a great influence on the electrophysical changes induced by Cu and this may be related to the speciation of the metal, according to its degree of hydroxylation. It will now be possible to compare the effects of toxic metal ions (Cd, Hg, Pb, etc..) or therapeutic metal ions (Pt, Ga, Ru, Ti, Sn, etc..) with those observed for Cu, in the conditions optimized by this study.