RESUMEN
The effect of the preparation of colicin V, freed from the endotoxin of the producer strain E. coli Ca-7 on Sh. sonnei was studied in the keratoconjunctival tests on guinea pigs and in the cell culture Hep-2. Colicin V was found to inhibit the development of dysenteric infection in vivo by delaying the incubation period. It has been demonstrated that the mentioned colicin blocks the process of adsorption of the causative agent on the surface of epithelial cells. Obviously, colicin V can also display bactericidal effect at the moment of intercellular migration of Shigellae, but it has no effect on bacteria which have penetrated into the cytoplasm of epithelial cells. The obtained data support the opinion of the role of colicins played in the development and course of infectious processes.
Asunto(s)
Colicinas/farmacología , Shigella sonnei/efectos de los fármacos , Animales , Línea Celular , Supervivencia Celular/efectos de los fármacos , Colicinas/uso terapéutico , Disentería Bacilar/tratamiento farmacológico , Escherichia coli/efectos de los fármacos , Cobayas , Técnicas In Vitro , Queratoconjuntivitis/tratamiento farmacológico , Especificidad de la EspecieRESUMEN
A model on a HEp-2 cell culture was elaborated, permitting the study of the ability of microbes to be adsorbed an to proliferate on the surface of cells and of the mechanism of their protective effect. The ability of E. coli strains to be adsorbed and to proliferate on the surface of a cell culture was found to differ. It has been demonstrated that the protection of the cell culture from subsequent infection with virulent Shigella can be explained not only by the antagonistic activity of E. coli strains, but also by their ability to be adsorbed and to proliferate on the surface of cells. A similar mechanism of protective effect is supposed in preparations of the Colibacterin type.