RESUMEN

In this study, a single step in situ sol-gel method was used to syntheses nanocomposite films using chitosan (CS) as the basis material, with the addition of silver oxide nanoparticles (Ag2O) at several weight percentages (5 %, 10 %, and 15 % Ag2O/CS). The structural characteristics of Ag2O/CS films were investigated using a range of analytical techniques. The presence of the primary distinctive peaks of chitosan was verified using FTIR spectra analysis. However, a minor displacement was observed in these peaks due to the chemical interaction occurring with silver oxide molecules. XRD analysis demonstrated a significant increase in the crystallinity of chitosan when it interacted with metal oxide nanoparticles. Furthermore, it is believed that the interaction between silver oxide and the active binding sites of chitosan is responsible for the evenly dispersed clusters shown in the micrographs of the chitosan surface, as well as the random aggregations within the pores. EDS technique successfully identified the presence of distinctive silver signals within the nanocomposite material, indicating the successful absorption of silver into the surface of the polymer. The developed Ag2O/CS nanocomposite showed promising antibacterial activity against Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis and Staphylococcus aureus). Also, Ag2O/CS nanocomposite exhibited marked antifungal activity against Candida albicans, Aspergillus flavus, A. fumigatus, A. niger, and Penicillium chrysogenum. The antioxidant activity of the developed nanocomposite films was studied by ABTS radical scavenging. The highest antioxidant and antibacterial properties were achieved by including 15 % silver oxide into the chitosan. Therefore, our finding indicate that chitosan­silver oxide nanocomposites exhibits significant potential as a viable material for application in several sectors of the food packaging industry.

Asunto(s)

Antiinfecciosos , Quitosano , Nanopartículas del Metal , Nanocompuestos , Óxidos , Compuestos de Plata , Quitosano/química , Antioxidantes/farmacología , Embalaje de Alimentos , Antiinfecciosos/farmacología , Antiinfecciosos/química , Antibacterianos/farmacología , Antibacterianos/química , Nanopartículas del Metal/química , Nanocompuestos/química

RESUMEN

Endophytic bacteria are plant-beneficial bacteria with a broad host range. They provide numerous benefits to their hosts, helping them tolerate several biotic and abiotic stresses. An interest has recently been developed in endophytic bacteria which are producing bioactive compounds that contribute to the biological control of various phytopathogens. This research paper aimed to investigate the potentiality of new local strains of endophytic bacteria such as Bacillus amyloliquefaciens and B. velezensis and the production of several antimicrobial metabolites associated with the biocontrol of Alternaria sp., which cause serious diseases and affect important vegetable crops in Egypt. Twenty-five endophytic bacteria isolates were obtained from different plants cultivated in El-Sharkia Governorate, Egypt. Dual culture technique was used to evaluate the bacterial isolates' antagonistic potentiality against Alternaria sp. and Helminthosporium sp. The most active bacterial isolates obtained were selected for further screening. The antifungal activity of the most active endophytic bacterial isolate was assessed in vivo on pepper seedlings as a biocontrol agent against Alternaria sp. A significant antifungal activity was recorded with isolates C1 and T5 against Alternaria sp. and Helminthosporium sp. The bacterial endophyte discs of C1 and T5 showed the highest inhibitory effect against Alternaria sp. at 4.7 and 3.1 cm, respectively, and Helminthosporium sp. at 3.9 and 4.0 cm, respectively. The most active endophytic isolates C1 and T5 were identified and the 16S rRNA sequence was submitted to the NCBI GenBank database with accession numbers: MZ945930 and MZ945929 for Bacillus amyloliquefaciens and Bacillus velezensis, respectively. The deformity of pathogenic fungal mycelia of Alternaria sp. and Helminthosporium sp. was studied under the biotic stress of bacteria. The culture filtrates of B. amyloliquefaciens and B. velezensis were extracted with different solvents, and the results indicated that hexane was the most efficient. Gas Chromatography-Mass Spectrometry revealed that Bis (2-ethylhexyl) phthalate, Bis (2-ethylhexyl) ester, and N,N-Dimethyldodecylamine were major constituents of the endophytic crude extracts obtained from B. amyloliquefaciens and B. velezensis. The in vivo results showed that Alternaria sp. infection caused the highest disease incidence, leading to a high reduction in plant height and in the fresh and dry weights of pepper plants. With B. amyloliquefaciens application, DI significantly diminished compared to Alternaria sp. infected pepper plants, resulting in an increase in their morphological parameters. Our findings allow for a reduction of chemical pesticide use and the control of some important plant diseases.

RESUMEN

Fusarium solani, the causative agent of root rot disease is one of the major constraints of faba bean (Vicia faba L.) yield worldwide. Essential oils have become excellent plant growth stimulators besides their antifungal properties. Foeniculum vulgare Mill. (fennel) is a familiar medicinal plant that has inhibitory effects against phytopathogenic fungi. Herein, different concentrations of fennel seed essential oil (FSEO) (12.5, 25, 50, 100, 200 and 400 µL/mL) were examined against F. solani KHA10 (accession number MW444555) isolated from rotted roots of faba bean in vitro and in vivo. The chemical composition of FSEO, through gas chromatography/mass spectroscopy, revealed 10 major compounds. In vitro, FSEO inhibited F. solani with a minimum inhibitory concentration (MIC) of 25 µL/mL. In vivo, FSEO suppressed Fusarium root rot disease in Vicia faba L. by decreasing the disease severity (61.2%) and disease incidence (50%), and acted as protective agent (32.5%) of Vicia faba L. Improvements in morphological and biochemical parameters were recorded in FSEO-treated faba seeds. Moreover, the expression level of the defense-related genes defensin and chitinase was noticeably enhanced in treated plants. This study suggested using FSEO as a promising antifungal agent against F. solani not only to control root rot disease but also to enhance plant growth and activate plant defense.

RESUMEN

Rhizoctonia root-rot disease causes severe economic losses in a wide range of crops, including Vicia faba worldwide. Currently, biosynthesized nanoparticles have become super-growth promoters as well as antifungal agents. In this study, biosynthesized selenium nanoparticles (Se-NPs) have been examined as growth promoters as well as antifungal agents against Rhizoctonia solani RCMB 031001 in vitro and in vivo. Se-NPs were synthesized biologically by Bacillus megaterium ATCC 55000 and characterized by using UV-Vis spectroscopy, XRD, dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. TEM and DLS images showed that Se-NPs are mono-dispersed spheres with a mean diameter of 41.2 nm. Se-NPs improved healthy Vicia faba cv. Giza 716 seed germination, morphological, metabolic indicators, and yield. Furthermore, Se-NPs exhibited influential antifungal activity against R. solani in vitro as well as in vivo. Results revealed that minimum inhibition and minimum fungicidal concentrations of Se-NPs were 0.0625 and 1 mM, respectively. Moreover, Se-NPs were able to decrease the pre-and post-emergence of R. solani damping-off and minimize the severity of root rot disease. The most effective treatment method is found when soaking and spraying were used with each other followed by spraying and then soaking individually. Likewise, Se-NPs improve morphological and metabolic indicators and yield significantly compared with infected control. In conclusion, biosynthesized Se-NPs by B. megaterium ATCC 55000 are a promising and effective agent against R. solani damping-off and root rot diseases in Vicia faba as well as plant growth inducer.