RESUMEN
BACKGROUND: Traditionally Trichodesma indicum has been used for its therapeutic effect in folk medicine that include anti-inflammatory, analgesic and anticancer properties. In this work, we validate the anticancer potential of the plant. AIMS: To screen the shoot extracts T. indicum for their antimitotic and antiproliferative activities. MATERIALS AND METHODS: The dried aerial parts of T. indicum were successively extracted with petroleum ether, successive chloroform extract (SCH), successive ethanol extract (SEE) and water. The plant extracts were subjected to study of in vitro antioxidant activity using 2,2'- diphenyl-1-picrylhydrazyl, 2,2'- azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical inhibition systems. The extracts were also tested for their in vitro antimitotic activity in Allium cepa root and antiproliferative activity using the yeast model and five human cell lines (MCF-7, HOP-62, MOLT-4, HCT-15 and PRO). RESULT AND CONCLUSION: The mitotic index for SCH and SEE was found to be 12.01 ± 1.34 and 12.99 ± 0.25 mg/mL, respectively. The IC50 value in the antiproliferative assay was found to be 30.14s-35.36 mg/mL for SCH and SEE respectively. Both SCH and SEE extracts showed significant antimitotic and antiproliferative activity when compared to the standard methothreaxate, vincreastine and adriamycin. Among the extracts, SEE showed strong inhibition against MCF-7 and MOLT-4 cell lines at concentration <30 µg/mL. Phytochemical analysis of extracts indicated the presence of ß-sitosterol, gallic acid and catechin. Based on these results, it is concluded that T. indicum may be a good candidate for the treatment of a variety of cancer. Thus, its traditional use is validated.
RESUMEN
BACKGROUND: The present communication deals with the identification and characterization of bioactive principles from the roots of Chlorophytum borivilianum. METHOD: Methanolic extract and its fractions were used to isolate different phytoconstituents. The structures of isolated compounds were characterized and elucidated with chemical and spectroscopic techniques such as Infra Red, Nuclear Mass Resonace and Mass spectroscopy experiments. Fatty acids were characterized by GC-MS analysis. RESULT: Three Fatty acids were isolated and confirmed. One sterol stigmasterol was isolated. One new saponin named as Chlorophytoside-I (3ß, 5α, 22R, 25R)-26-(ß-D-glucopyranosyloxy)-22-hydroxy-furostan-12-one-3 yl O-ß-D-galactopyranosyl (1-4) glucopyranoside was isolated. CONCLUSION: The roots of Chlorophytum borivilianum contain three important fatty acids, common sterol stigmasterol and one furostanol saponins.