RESUMEN
The present study investigated the indigenous metal-tolerant bacterial populations in the mine-water microbiome. Our intention was to assess the effects of the metal concentrations in mine water on the bacterial community of mine waters. The bacterial communities in Vanadium and Gold mine-water samples were exposed to different heavy-metal Arsenic, Cadmium, Chromium, Nickel, Mercury and Vanadium at two different concentrations (5 and 25 mM). The 16S rRNA amplicon from mine waters were sequenced using the Illumina's NGS MiSeq platform. Data analysis revealed a high diversity in the bacterial populations associated with the different heavy metals at different concentrations. The taxonomic profiles obtained after the exposure were different in different salts, but mostly dominated by Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Firmicutes at variable relative abundance. Principal Component Analysis (PCoA) predicts the clear community shift after exposure with heavy metals salts and emergence of tolerant community depending upon the specific community present in the original mine water.
Asunto(s)
Bacterias , Metales Pesados , Minería , Contaminantes Químicos del Agua , Metales Pesados/análisis , Metales Pesados/toxicidad , Contaminantes Químicos del Agua/análisis , Bacterias/efectos de los fármacos , Bacterias/genética , Bacterias/clasificación , Microbiota/efectos de los fármacos , ARN Ribosómico 16S , Microbiología del Agua , Monitoreo del AmbienteRESUMEN
Limited data exist on the simultaneous impact of bottom-up (nutrients) and top-down (viruses and heterotrophic nanoflagellates) forces in shaping freshwater bacterial communities. In our laboratory microcosms, nutrient additions (organic and inorganic) and viral reduction approach led to the proliferation of high nucleic acid (HNA) bacterial subpopulation without an increase in phage abundance. High viral-mediated bacterial lysis in the presence of nanoflagellates yielded high proportion of low nucleic acid bacterial subpopulation. 16S rRNA gene sequence analysis indicated that members of classes Proteobacteria and Bacteroidetes evoked differential responses to nutrients and mortality forces, thereby resulting in differences (P < 0.001) in bacterial community composition and diversity, as observed from analysis of similarities and UniFrac analysis. Bacterial species richness (Chao) and diversity (Shannon) index was significantly higher (P < 0.001) in the presence of both the top-down factors and viruses alone, whereas lower host diversity was observed under nutrient relaxation of growth-limiting substrates due to the explosive growth of opportunistic HNA bacterial subpopulation. Our results are in agreement with the theoretical model of 'killing the winner', where the availability of growth-limiting substrates can act as a stimulating factor for host community composition while top-down forces can operate in the control of host diversity.
Asunto(s)
Agua Dulce/microbiología , Microbiota , Microbiología del Agua , Bacterias/genética , Bacteriófagos/genética , Procesos Heterotróficos , ARN Ribosómico 16S , Virus/genéticaRESUMEN
We examined the relationship between viruses and co-occurring bacterial communities across spatiotemporal scale in two contrasting freshwater lakes, namely meromictic Lake Pavin and dimictic Lake Aydat (Central France). Next-generation sequencing of 16S rRNA genes suggested distinct patterns in bacterioplankton community composition (BCC) between the lakes over depths and seasons. BCC were generally dominated by members of Actinobacteria, Proteobacteria, and Bacteroidetes covering about 95% of all sequences. Oxygen depletion at the bottom waters in Aydat and existence of permanent anoxia in the monimolimnion of Pavin resulted in the occurrence and dominance of lesser known members of lake communities such as Methylotenera, Methylobacter, Gallionella, Sulfurimonas, and Syntrophus in Pavin and Methylotenera and Sulfuritalea in Aydat. Differences in BCC appeared strongly related to dissolved oxygen concentration, temperature, viral infection, and virus-to-bacteria ratio. UniFrac analysis indicated a clear distinction in BCC when the percentage of viral infected bacterial cells and virus-to-bacteria ratio exceeded a threshold level of 10% and 5, respectively, suggesting a link between viruses and their potential bacterial host communities. Our study revealed that in both the lakes, the prevailing environmental factors across time and space structured and influenced the adaptation of bacterial communities to specific ecological niches.
Asunto(s)
Bacterias/clasificación , Lagos/microbiología , Filogenia , Plancton/clasificación , Microbiología del Agua , Actinobacteria/clasificación , Actinobacteria/genética , Bacterias/genética , Bacteriófagos , Bacteroidetes/clasificación , Bacteroidetes/genética , Biodiversidad , Ecosistema , Francia , Lagos/química , Microbiota/genética , Plancton/genética , Proteobacteria/clasificación , Proteobacteria/genética , ARN Ribosómico 16S/genética , Estaciones del Año , TemperaturaRESUMEN
Bacteria assemblages in lake sediments play a key role in various biogeochemical processes, yet their association with interstitial pore waters has been scarcely investigated. In this study, we utilized Illumina next-generation amplicon sequencing of the 16S rRNA gene to characterize the seasonal bacterial communities in the sediments and pore waters of three contrasted temperate freshwater lakes, namely Pavin, Aydat, and Grangent (French Massif Central). Despite occupying seemingly similar habitats, bacterial communities differed substantially between sediments and pore waters at all seasons with low sharing of operational taxonomic units (OTUs, 6.7 to 20.3%) between them. Sediment-associated bacteria were more rich and diverse than pore water bacteria, indicating a high heterogeneity in the sediment microhabitat. The changes in both sediment and pore water bacterial communities were lake and season specific. The bacterial community showed distinct differences between the lakes, with larger presence of strict anaerobes such as Syntrophus, Syntrophorhabdus, and Sulfuricurvum in the pore water and sediments of Pavin responsible for carbon and sulfur cycling. In both Aydat and Grangent, the hgcI_clade dominated throughout the study period in the pore waters. The higher representation of lesser-known transient members of lake communities such as Methylotenera in the pore waters of Aydat, and Clostridium and Sulfuricurvum in the pore and sediments of Grangent, respectively, were observed during the period of temporary anoxia in summer caused by lake stratification. Our study revealed that in the investigated lakes, the prevailing environmental factors across time and space structured and influenced the adaptation of bacterial communities to specific ecological niches.
Asunto(s)
Bacterias/clasificación , Agua Dulce/microbiología , Sedimentos Geológicos/microbiología , Lagos/microbiología , Filogenia , Plancton/clasificación , Microbiología del Agua , Adaptación Biológica , Organismos Acuáticos , Bacterias/genética , Bacterias/aislamiento & purificación , Biodiversidad , Carbono/metabolismo , ADN Bacteriano/genética , Ecosistema , Francia , Agua Dulce/química , Secuenciación de Nucleótidos de Alto Rendimiento , Lagos/química , Microbiota , Plancton/genética , Plancton/aislamiento & purificación , ARN Ribosómico 16S/genética , Estaciones del Año , Análisis de Secuencia de ADN , Azufre/metabolismo , Virus/clasificaciónRESUMEN
The expected growth in nanomaterial applications could result in increased amounts of nanoparticles entering municipal sewer systems, eventually ending up in wastewater treatment plants and therefore negatively affecting microbial populations and biological nutrient removal. The aim of this study was to ascertain the impact of zinc oxide nanoparticles (nZnO) on the bacterial microbiome of an activated sludge system. A metagenomic approach combined with the latest generation Illumina MiSeq platform and RDP pipeline tools were used to identify and classify the bacterial microbiome of the sludge. Results revealed a drastic decrease in the number of operational taxonomic units (OTUs) from 27 737 recovered in the nZnO-free sample to 23 743, 17 733, and 13 324 OTUs in wastewater samples exposed to various concentrations of nZnO (5, 10 and 100 mg/L nZnO, respectively). These represented 12 phyla, 21 classes, 30 orders, 54 families and 51 genera, completely identified at each taxonomic level in the control samples; 7-15-25-28-20 for wastewater samples exposed to 5 mg/L nZnO; 9-15-24-31-23 for those exposed to 10 mg/L and 7-11-19-26-17 for those exposed 100 mg/L nZnO. A large number of sequences could not be assigned to specific taxa, suggesting a possibility of novel species to be discovered.
Asunto(s)
Bacterias/clasificación , Aguas del Alcantarillado/microbiología , Óxido de Zinc/farmacología , Bacterias/efectos de los fármacos , Bacterias/genética , ADN Bacteriano/genética , Secuenciación de Nucleótidos de Alto Rendimiento , Metagenómica , Nanopartículas/química , Filogenia , Análisis de Secuencia de ADN , Aguas Residuales/microbiologíaRESUMEN
We conducted an experimental approach using microcosms to simultaneously examine the functional response of natural freshwater bacterial assemblages to the impact of resources (nutrients) and top-down factors (viruses and grazers) on bacterial physiological state and their community structure. Addition of organic and inorganic nutrients led to the proliferation of high nucleic acid content bacterial cells accompanied by high bacterial growth efficiency (considered as proxy of bacterial carbon metabolism) estimates, suggesting that this subgroup represented the most active fraction of bacterial community and had a high capacity to incorporate carbon into its biomass. However, their rapid growth induced the pressure of viral lytic infection which led to their lysis toward the end of the experiment. In microcosms with flagellates plus viruses, and with viruses alone, the selective removal of metabolically active high nucleic acid cells through viral lysis benefitted the less active low nucleic acid content cells, perhaps via the use of lysis products for its growth and survival. Changes in bacterial physiological state in microcosms were reflected in their community structure which was examined using 16S ribosomal RNA (rRNA) gene sequencing by Illumina's Miseq platform. Chao estimator and Shannon diversity index values suggested that bacterial species richness was highest in the presence of both the top-down factors, indicating a tighter control of bacterioplankton dominants within a relatively stable bacterial community. The increase in bacterial metabolism with nutrient addition followed by subsequent lysis of bacterial dominants indicate that both resources and top-down factors work in concert for the sustenance of stable bacterial communities.