RESUMEN
Bemisia tabaci is a global invasive pest causing substantial loss on several economically important crops and has developed a very high level of resistance to insecticides making current management practices ineffective. Thus, the novel pest management strategy like RNA interference (RNAi) has emerged as a potential molecular tool in the management of insect pests particularly B. tabaci. The present study investigated RNAi mediated silencing of the Ecdysone Receptor (EcR) gene in B. tabaci Asia-I using biodegradable Chitosan Nanoparticles (CNPs) hydrogel containing EcR dsRNA. The formation of nanohydrogel and dsRNA loading were characterized by gel retardation assay, scanning electron microscopy (SEM); transmission electron microscopy (TEM) and Fourier transform infrared microscopy (FTIR). The stability of CNPs/dsRNA was assessed by exposure to direct sunlight and UV light for different time periods. The CNPs/dsRNA exhibited increased stability over the untreated control and further confirmed by bioassay studies which yielded mortality over 80% and effectively down regulated the expression of the EcR gene as confirmed by qRT-PCR analysis. These investigations provide potential avenues for advancing innovative pest management strategies using biopolymer CNPs hydrogel, which can enhance the efficiency of dsRNA as a safe and targeted solution in the management of whiteflies.
Asunto(s)
Quitosano , Hemípteros , Receptores de Esteroides , Animales , Quitosano/farmacología , Quitosano/metabolismo , Hemípteros/genética , Hemípteros/metabolismo , ARN Bicatenario/genética , ARN Bicatenario/metabolismo , Interferencia de ARN , Hidrogeles/metabolismoRESUMEN
The reproductive success of herbivorous insects largely depends on the mother's oviposition preference. In nocturnal insects, olfaction is arguably the most important sensory modality mediating mate finding, foraging, and host location. In most habitats, gravid females select among a number of plants of varying suitability, yet assessment of the neuroethological mechanisms underlying odor-guided choice between host plants is rare. Using a series of behavioral, electrophysiological, and chromatographic analyses in the Hawk moth, Manduca sexta, we show that gravid females perform a hierarchical choice among host plants of different species and qualities using olfactory cues. Both relevant plant species and qualities can be distinguished by volatile profiles collected from the headspace of these plants, and olfactory sensilla on female antennae detect more than half of the about 120 analytically detected volatiles in host plant headspace samples. Although olfactory sensory neurons present in antennal sensilla are mainly broadly tuned to multiple host compounds, some sensilla exhibit species and condition-specific responses. In fact, species and quality can be distinguished by the physiologically active components alone. Our findings thus suggest that distinguishing characteristics of both host species and quality are already represented at the sensory periphery.