RESUMEN
OBJECTIVES: To determine whether the oxidative stress transmitted to newly grown hair from an unhealthy scalp has physical consequences to the cuticular condition and function. METHODS: A uniquely designed 24-week clinical study included 8 weeks of pretreatment with a cosmetic shampoo and 16 weeks of treatment with either a potentiated zinc pyrithione (ZPT) antidandruff shampoo or a placebo cosmetic shampoo. This clinical design allowed the growth and acquisition of hair samples under conditions of varying but known scalp health as a result of treating a dandruff/seborrheic dermatitis (D/SD) population. Two complementary methods were used to characterize the integrity of the cuticular surface. Hair surface hydrophobicity was assessed by quantifying water wetting force using a Wilhelmy balance method. Surface structure and porosity were assessed using dynamic vapor sorption (DVS) to gravimetrically quantify water sorption. RESULTS: Chemical oxidative stress to pre-emergent hair has been shown to have negative consequences to hair surface structure. Compared to a placebo shampoo control, use of a potentiated ZPT shampoo improved scalp health and significantly improved the following attributes associated with healthy hair: hair surface hydrophobicity (surface energy) and cuticular moisture barrier effectiveness (dynamic vapor sorption). CONCLUSIONS: Pre-emergent hair can be negatively impacted by the oxidative stress that occurs with an unhealthy scalp, possibly due to metabolic activity of resident microbes. Manifestations of the oxidative stress include altered cuticle surface properties that are responsible for its protective function; these effects are similar in type to those observed by bleaching post-emergent hair. These alterations have the potential to make the hair, once emerged from the scalp, more susceptible to the cumulative physical and chemical insults responsible for hair feel and look, fiber integrity, and overall retention.
Asunto(s)
Caspa/terapia , Dermatitis Seborreica/terapia , Preparaciones para el Cabello/uso terapéutico , Compuestos Organometálicos/uso terapéutico , Estrés Oxidativo/fisiología , Piridinas/uso terapéutico , Dermatosis del Cuero Cabelludo/terapia , Adolescente , Adulto , Anciano , Canadá , Dermatitis Seborreica/fisiopatología , Método Doble Ciego , Femenino , Folículo Piloso/efectos de los fármacos , Preparaciones para el Cabello/química , Humanos , Masculino , Persona de Mediana Edad , Valores de Referencia , Medición de Riesgo , Dermatosis del Cuero Cabelludo/fisiopatología , Índice de Severidad de la Enfermedad , Resultado del Tratamiento , Adulto JovenRESUMEN
Despite an increasing knowledge of dandruff and seborrheic dermatitis (D/SD), the pathophysiological understanding is still incomplete but suggests a role of Malassezia yeasts in triggering inflammatory and hyper-proliferative epidermal responses. The objective of this report is to review published literature from in vivo studies of D/SD populations to provide a more complete description of overall scalp health. New biomolecular capabilities establish a depth of pathophysiological understanding not previously achievable with traditional means of investigation. Biomarkers representing inflammation, hyper-proliferation and barrier function are all perturbed by the D/SD condition and robustly respond to therapeutic resolution. These biomarkers can be sampled noninvasively, enabling their use in routine clinical evaluations as either surrogate endpoints or complementary ones to classical signs/symptoms to broaden the etiological learning.
Asunto(s)
Dermatitis Seborreica/fisiopatología , Dermatomicosis/fisiopatología , Pitiriasis/fisiopatología , Dermatosis del Cuero Cabelludo/fisiopatología , Cuero Cabelludo/fisiopatología , Biomarcadores/metabolismo , Dermatitis Seborreica/metabolismo , Dermatitis Seborreica/patología , Dermatomicosis/metabolismo , Dermatomicosis/microbiología , Dermatomicosis/patología , Humanos , Malassezia/patogenicidad , Pitiriasis/metabolismo , Pitiriasis/patología , Valor Predictivo de las Pruebas , Pronóstico , Cuero Cabelludo/metabolismo , Cuero Cabelludo/microbiología , Cuero Cabelludo/patología , Dermatosis del Cuero Cabelludo/metabolismo , Dermatosis del Cuero Cabelludo/microbiología , Dermatosis del Cuero Cabelludo/patologíaRESUMEN
Dandruff and seborrhoeic dermatitis are accompanied by bothersome itch. We have established a novel non-invasive methodology to sample histamine levels in the stratum corneum in order to facilitate an understanding of pruritogenesis in this condition. Histamine levels were assessed in two groups of subjects with dandruff before and after 3 weeks of treatment with a commercial potentiated zinc pyrithione shampoo. A comparative population without dandruff was also studied. Itch self-perception was quantified on a visual analogue scale. The histamine level in subjects with dandruff was more than twice that in those who did not have dandruff. Under conditions known to resolve flaking symptoms, the shampoo led to a reduction in histamine in subjects with dandruff to a level that was statistically indistinguishable from those who did not have dandruff. This reduction in histamine was accompanied by a highly significant reduction in the perception of itch intensity. These findings suggest an association between the subjective perception of itch in the scalp and the level of histamine in the skin.
Asunto(s)
Antipruriginosos/administración & dosificación , Dermatitis Seborreica/tratamiento farmacológico , Preparaciones para el Cabello/administración & dosificación , Histamina/metabolismo , Queratolíticos/administración & dosificación , Compuestos Organometálicos/administración & dosificación , Prurito/tratamiento farmacológico , Piridinas/administración & dosificación , Dermatosis del Cuero Cabelludo/tratamiento farmacológico , Piel/efectos de los fármacos , Manejo de Especímenes , Administración Tópica , Adulto , Análisis de Varianza , Biomarcadores/metabolismo , Cromatografía Líquida de Alta Presión , Dermatitis Seborreica/metabolismo , Método Doble Ciego , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Masculino , Persona de Mediana Edad , Prurito/metabolismo , Cuero Cabelludo , Dermatosis del Cuero Cabelludo/metabolismo , Piel/metabolismo , Encuestas y Cuestionarios , Espectrometría de Masas en Tándem , Factores de Tiempo , Resultado del TratamientoRESUMEN
BACKGROUND: Dandruff is a common scalp condition characterized by flakes, pruritus and sometimes mild erythema. These symptoms reflect underlying histopathologic and biochemical events that must be reversed if treatment is to be effective. OBJECTIVES: This study aimed to better characterize the state of the epidermis in dandruff and to determine how a defined set of skin surface biomarkers of this state change during a successful course of treatment with a potentiated zinc pyrithione (ZPT) shampoo. METHODS: A population of dandruff sufferers was treated for 3 weeks with a commercial ZPT shampoo or a non-medicated product, and the effect of treatment on adherent scalp flake (ASF) scores was evaluated. Biopsies were taken from lesional sites at baseline and at the end of the study for histomorphometric and histopathologic analysis. Stratum corneum (SC) samples were likewise obtained for evaluation of biochemical markers of inflammation (IL-1α, IL-1RA, IL-8) and barrier integrity (keratin 1, 10, 11; involucrin; SC lipids; human serum albumin). The biomarker profile was evaluated first by comparison with that in non-dandruff subjects at baseline, and then to determine whether any treatment-induced changes were correlated with reductions in flaking in dandruff sufferers. RESULTS: Taken together, our studies showed that treatment with the ZPT shampoo led to an improvement in the overall scalp condition as assessed by the resolution of flaking, reduction in epidermal thickness and inflammatory biomarkers, and a dramatic improvement in biomarkers of epidermal barrier integrity. CONCLUSIONS: The combination of biomarkers examined appears to be a good overall descriptor of the health of the scalp in dandruff, and changes in these biomarkers track with tissue-level events that underlie clinical efficacy in the treatment of dandruff by ZPT shampoo. For the first time, we demonstrate a set of tools that extend beyond flaking scores to provide insight into specific biological changes occurring on the scalp to enable an objective assessment of scalp health.
Asunto(s)
Dermatitis Seborreica/tratamiento farmacológico , Epidermis/efectos de los fármacos , Preparaciones para el Cabello/uso terapéutico , Cuero Cabelludo/efectos de los fármacos , Adolescente , Adulto , Anciano , Biomarcadores/metabolismo , Biopsia , Femenino , Humanos , Inflamación/metabolismo , Interleucinas/metabolismo , Queratinas/metabolismo , Masculino , Persona de Mediana Edad , Compuestos Organometálicos/uso terapéutico , Piridinas/uso terapéutico , Resultado del Tratamiento , Adulto JovenRESUMEN
Culturing has detected vaginal Staphylococcus aureus in 10%-20% of women. Because growth mode can affect virulence expression, this study examined S. aureus-biofilm occurrence in 44 paired-tampon and vaginal-wash-specimens from 18 prescreened women, using fluorescent in situ hybridization (FISH). All 44 specimens were also analyzed for S. aureus by standard culturing on mannitol salt agar, which produced positive results for 15 of the 44 specimens. FISH detected S. aureus cells in all 44 specimens, and S. aureus biofilm was observed in 37 of the 44 specimens. Independent confirmation of the presence of S. aureus in specimens from all 18 women was also obtained by amplification, via polymerase chain reaction, of an S. aureus-specific nuclease gene. The results of this study demonstrate that S. aureus biofilm can form on tampons and menses components in vivo. Additionally, the prevalence of vaginal S. aureus carriage may be more prevalent than what is currently demonstrated by standard culturing techniques.
Asunto(s)
Biopelículas/crecimiento & desarrollo , Productos para la Higiene Menstrual/microbiología , Menstruación , Staphylococcus aureus/aislamiento & purificación , Vagina/microbiología , Adolescente , Adulto , Portador Sano/diagnóstico , Portador Sano/microbiología , Femenino , Humanos , Hibridación Fluorescente in SituRESUMEN
Poly(3-hydroxybutyrate-co-3-hydroxyoctanoate), PHBO, represents a class of PHA copolymers that contain both short-chain-length and medium-chain-length repeat units. Radiolabeled and cold PHBO, containing 90 mol % 3-hydroxybutyrate and 10 mol % 3-hydroxyoctanoate were chemically synthesized using a new difunctional alkoxyzinc initiator. (14)C-PHBO was incubated with samples of anaerobic digester sludge, septage, freshwater sediment, and marine sediment under conditions resembling those in situ. In addition, it was incubated in laboratory-scale landfill reactors. (14)C-PCL (poly-epsilon-caprolactone) was incubated with anaerobic digester sludge and in landfill reactors. Biodegradation was determined by measuring generation of (14)CO(2) and (14)CH(4) resulting from mineralization of the radiolabeled polymers. PHBO was extensively mineralized in digester sludge, septage sediments, and the landfill reactors, with half-lives less than 30 days. PCL was not significantly mineralized in digester sludge over 122 days. In the landfill reactors, PCL mineralization was slow and was preceded by a long lag period (>200 days), suggesting that PCL mineralization is limited by its rate of hydrolysis. The results indicate that PHBO is practically biodegradable in the major anaerobic habitats that it may enter. In contrast, anaerobic biodegradation of PCL is less ubiquitous and much slower.