RESUMEN
4-Ene-3-ketosteroids and 17-ketosteroids were quantitatively transformed into the corresponding hydrazones using Girard P and T reagents, respectively. The positively charged derivatives were separated by capillary electrophoresis. The spectrophotometric characteristics of the derivatives permitted their sensitive detection in the 230-280 nm range. The steroids investigated included nortestosterone and its phenylpropionate, norethisterone and its oenanthate, d,l-norgestrel, dehydroepiandrosterone, androstenedione and ethisterone.
Asunto(s)
Cetosteroides/aislamiento & purificación , Androstenodiona/análogos & derivados , Androstenodiona/análisis , Androstenodiona/aislamiento & purificación , Betaína/análogos & derivados , Betaína/química , Deshidroepiandrosterona/análogos & derivados , Deshidroepiandrosterona/análisis , Deshidroepiandrosterona/aislamiento & purificación , Electroforesis Capilar , Etisterona/análogos & derivados , Etisterona/análisis , Etisterona/aislamiento & purificación , Indicadores y Reactivos/química , Cetosteroides/análisis , Nandrolona/análogos & derivados , Nandrolona/análisis , Nandrolona/aislamiento & purificación , Noretindrona/análogos & derivados , Noretindrona/análisis , Noretindrona/aislamiento & purificación , Norgestrel/análogos & derivados , Norgestrel/análisis , Norgestrel/aislamiento & purificación , Espectrofotometría UltravioletaRESUMEN
A normal-phase ion-pair chromatographic system has been developed for the high-performance liquid chromatographic investigation of pipecuronium bromide (2 beta,16 beta-bis-(N'-dimethyl-l-piperazinyl)-3 alpha,17 beta-diacetoxy-5 alpha-androstane dibromide) and related quaternary ammonium steroids. The use of silica as the stationary phase and a 96:4 mixture of acetonitrile and water containing 0.1 mol/dm3 sodium perchlorate as the eluent with detection at 213 nm enable the potential impurities as well as the hydrolytic and oxidative degradation products of pipecuronium bromide to be separated and detected down to the 0.01% level. The above system is also applicable to the high-performance liquid chromatographic investigation of other quaternary ammonium steroids (pancuronium bromide, vecuronium bromide).