RESUMEN

An ELISA was compared with the plaque-reduction serum neutralization (PRSN) test, for detection of vesicular stomatitis virus (VSV) antibodies in cattle in a vesicular stomatitis enzootic region of Mexico. A total of 325 bovine serum samples were screened for VSV antibodies. The PRSN test was performed, using Vero cells. The ELISA contained gradient-purified VSV Indiana (Lab strain) and VSV New Jersey (Hazelhurst) as the antigens. Regression analysis and weighted kappa statistic were used to estimate measures of agreement between the 2 assays for detection of VSV antibodies. The ELISA method proved useful for serodiagnosis of vesicular stomatitis. The ELISA and PRSN test results were highly correlated for detection of VSV antibodies.

Asunto(s)

Anticuerpos Antivirales/sangre , Enfermedades de los Bovinos/diagnóstico , Estomatitis/veterinaria , Virus de la Estomatitis Vesicular Indiana/inmunología , Vesiculovirus , Virosis/veterinaria , Animales , Antígenos Virales/inmunología , Bovinos , Enfermedades de los Bovinos/epidemiología , Ensayo de Inmunoadsorción Enzimática , Estudios de Evaluación como Asunto , México/epidemiología , Pruebas de Neutralización , Valor Predictivo de las Pruebas , Análisis de Regresión , Reproducibilidad de los Resultados , Estomatitis/diagnóstico , Estomatitis/epidemiología , Células Vero , Virosis/diagnóstico , Virosis/epidemiología