RESUMEN

Multienzyme multiplex PCR-amplified fragment length polymorphism (ME-AFLP) typing is a reliable and simple method for typing of bacterial species. In this study we analyzed two well-documented strain collections of Staphylococcus aureus and compared ME-AFLP typing results with results of various other typing methods. The discriminatory power of ME-AFLP was found comparable to pulsed-field gel electrophoresis, and typing results were highly concordant. ME-AFLP typing presents a suitable method for prescreening of large strain collections. Furthermore, the obtained typing patterns were found to cluster according to the staphylococcal cassette chromosome mec types of the strains.

Asunto(s)

Electroforesis en Gel de Campo Pulsado/métodos , Resistencia a la Meticilina , Reacción en Cadena de la Polimerasa/métodos , Polimorfismo de Longitud del Fragmento de Restricción , Staphylococcus aureus/clasificación , Staphylococcus aureus/efectos de los fármacos , Proteínas Bacterianas/genética , Técnicas de Tipificación Bacteriana , Cromosomas Bacterianos , Brotes de Enfermedades , Humanos , Infecciones Estafilocócicas/epidemiología , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/genética