RESUMEN
An immunohistochemical study using antibodies against Ki-67 protein was conducted, which characterized the proliferative activity of cells and matrix metalloproteinase-9 (MMP-9) in follicular cyst, variants of keratocystic odontogenic tumor (with a preponderance of hyperkeratosis and parakeratosis), and ameloblastoma. The marked proliferative activity of a parabasal cell layer (28.0+/-8.4%) was found in the keratocystic odontogenic tumor with a preponderance of parakeratosis; the proliferative activity of the peripheral layer of ameloblastoma cells was equal to 7.0+/-5.6%. The maximal matrix metalloproteinase-9 expression in the keratocystic odontogenic tumor with a predominance of hyperkeratosis was 1.1 +/-0.9 conventional units (CU) and that in the ameloblastoma was 1.9+/-1.3 CU versus 0.4+/-0.5 CU in the follicular cyst, keratocystic odontogenic tumor with a preponderance of hyperkeratosis. The values of Ki-67 and MMP-9 expression allow one to distinguish benign odontogenic cysts and tumors (follicular cyst and keratocystic odontogenic tumor with a predominance of hyperkeratosis) and odontogenic tumors characterized by an aggressive clinical course (keratocystic odontogenic tumor with a preponderance of parakeratosis and ameloblastoma).
Asunto(s)
Ameloblastoma , Quiste Folicular , Regulación Neoplásica de la Expresión Génica , Antígeno Ki-67/biosíntesis , Metaloproteinasa 9 de la Matriz/biosíntesis , Quistes Odontogénicos , Adolescente , Adulto , Ameloblastoma/metabolismo , Ameloblastoma/patología , Niño , Diagnóstico Diferencial , Femenino , Quiste Folicular/metabolismo , Quiste Folicular/patología , Humanos , Masculino , Persona de Mediana Edad , Quistes Odontogénicos/metabolismo , Quistes Odontogénicos/patologíaRESUMEN
The immunohistochemical study of proliferation and intermediate filament--keratin 8 expressions at oral hyperkeratosis and oral Squamous cell carcinoma (OSCC) has been carried out. It has been shown, that the specific character of keratin 8 expressions can be used in differential diagnosis between oral hyperkeratosis and OSCC. Different types of oral hyperkeratosis (without atypia, SIN1, SIN2 and SIN3) can be differentiated by Ki-67 and keratin 8. We have found the moderate positive correlation between proliferation and a keratin 8 expression in neoplastic epithelial cells.
Asunto(s)
Biomarcadores de Tumor/biosíntesis , Carcinoma de Células Escamosas/metabolismo , Queratina-8/biosíntesis , Leucoplasia Bucal/metabolismo , Mucosa Bucal/metabolismo , Neoplasias de la Boca/metabolismo , Biomarcadores de Tumor/genética , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/patología , Proliferación Celular , Diagnóstico Diferencial , Células Epiteliales/metabolismo , Células Epiteliales/patología , Femenino , Humanos , Inmunohistoquímica , Queratina-8/genética , Antígeno Ki-67/biosíntesis , Leucoplasia Bucal/diagnóstico , Leucoplasia Bucal/patología , Masculino , Persona de Mediana Edad , Mucosa Bucal/patología , Neoplasias de la Boca/diagnóstico , Neoplasias de la Boca/patologíaRESUMEN
There were presented results of the study directed to disclosure of malignant cell changes criterion in cases of oral mucous membrane leukoplakia with different degrees of neoplastic transformation according to WHO-2005 classification of squamous intraepithelial neoplasia (SIN). With the help of immunohistochemical method proliferation in different layers of oral mucous membrane epithelium was evaluated. It was established that most important for diagnostics was the correspondence of proliferating cells in parabasal and basal epithelium layers. Figure less than 1 was corresponding to normal epithelium and SIN1, between 1 and 2 was corresponding to SIN2, 2 and more was characteristic itoSIN3.