RESUMEN
Various means for changing the aggregative stability of emulsions in the system of benzylpenicillin fermentation broth filtrate and butyl acetate were tested. It was shown that the use of desemulgators was more efficient than the use of some means for removing the desemulgating admixtures. Moreover, addition of surface active substances after the preliminary thermocoagulation resulted in maximum decreasing of the emulsion aggregative stability.
Asunto(s)
Penicilina G/aislamiento & purificación , Acetatos/farmacología , Medios de Cultivo , Estabilidad de Medicamentos , Emulsiones , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Penicilina G/administración & dosificación , Soluciones , Solventes/farmacología , Temperatura , Ultrafiltración/métodosRESUMEN
The separating capacity of a laboratory cup centrifuge and industrial centrifugal separator was compared. It was shown that the process of emulsion centrifugal separation could be modeled under laboratory conditions: antibiotic fermentation broth filtrate--organic solvent in industrial apparatus under similar conditions of preliminary mixing aqueous and organic phases.
Asunto(s)
Antibacterianos/aislamiento & purificación , Emulsiones , Eritromicina/aislamiento & purificación , SolucionesRESUMEN
Centrifugal extractors of the differential-contact type are widely used all over the world in production of antibiotics. Lately, new extractors were developed by Hitachi (Japan). Model E is of a special interest. In the present study the values of the working zone of the Hitachi extractor, EP-02 Ultrex were determined by the pressure levels and the effect of the main parameters characterizing the apparatus hydrodynamic conditions on the minimum and maximum critical pressure was estimated. The results of the study provide wide variation of the hydrodynamic parameters in determination of the most rational extraction conditions.