RESUMEN
A cDNA clone, designated Am-FaPS-1 (1310 bp), was isolated from callus culture derived from the leaf tissues of Aquilaria microcarpa. This gene contains an open reading frame encoding the protein of 342 amino acid residues with high homology to farnesyl diphosphate synthase from various plant sources. An appreciable increase in the transcriptional level of Am-FaPS-1 was reproducibly observed by the exposure of the cell culture to methyl jasmonate. The expression activity of the gene was also elevated when the cells were treated with yeast extract and Ca(2+)-ionophore A23187. These results suggest that Am-FaPS-1 and its translate play roles in methyl jasmonate- and yeast extract-induced responses of A. microcarpa, and Ca(2+) functions as an important messenger molecule in these processes. This set of the results would support our hypothesis that the activation of Ca(2+)-cascade evoked by the elevation of cytoplasmic Ca(2+) concentration is an essential early event in methyl jasmonate-induced responses of higher plant cells.
Asunto(s)
Acetatos/farmacología , Ciclopentanos/farmacología , Geraniltranstransferasa/metabolismo , Oxilipinas/farmacología , Proteínas de Plantas/metabolismo , Thymelaeaceae/enzimología , Secuencia de Aminoácidos , Calcio/metabolismo , ADN Complementario , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/genética , Geraniltranstransferasa/química , Geraniltranstransferasa/clasificación , Geraniltranstransferasa/genética , Datos de Secuencia Molecular , Proteínas de Plantas/química , Proteínas de Plantas/clasificación , Proteínas de Plantas/genética , Homología de Secuencia de Aminoácido , Thymelaeaceae/genéticaRESUMEN
A homology-based cloning strategy yielded two cDNA clones designated Am-cam-1 and Am-cam-2, presumably encoding calmodulin protein from a callus culture derived from the leaf tissues of Aquilaria microcarpa. An appreciable increase in the transcriptional activity of Am-cam-1 was reproducibly observed by exposure of the cell culture to methyl jasmonate, as analyzed by a reverse-transcription polymerase chain reaction. The expression level of the gene also increased when the cells were treated with yeast extract. The transcription of Am-cam-2 was similarly stimulated by the treatment with methyl jasmonate and yeast extract, however, the intensities of the enhanced expression appeared to be lower as compared with that of Am-cam-1. In contrast, Ca(2+)-ionophore A23187 did not show inducing activity for the expression of these two calmodulin genes. These results suggest that Am-cam-1 and Am-cam-2 and their products play important roles in signal transduction processes in methyl jasmonate- and yeast extract-treated cells of A. microcarpa, accompanying the change in the transcriptional activities.