RESUMEN
Ten biochemical parameters total protein, albumin, glucose, cholesterol, urea, creatinine, total bilirubin, ALT, AST, APh were determined during long-term light mode changes in serum of rats. Changing the light mode, a number of parameters revealed unreliable 24-hour rhythms. An ultradian 12-hour reliable rhythm has been for serum total protein of rats exposed to constant darkness during 1 month. The light-modified model can be used to study the effects of the chemical factor in combination with the physical factor of the external environment, as well as in preclinical toxicity tests of medicinal substances in different light modes.
Asunto(s)
Fenómenos Biomecánicos , Animales , Colesterol , Creatinina , Glucosa , RatasRESUMEN
This article describes models for the study of acute desynchronosis: jetlag syndrome and acute desynchronosis under physical stress for possible pharmacological correction of these disorders. The cosinor analysis allowed assessing significance of changes in biological rhythms in 2 biological models: the jetlag-type diurnal rhythm shift model and the model with changed light mode. The revealed changes in the rhythms of biochemical parameters in the blood serum of animals with acute desynchronosis indicate significant changes in the intensity of carbohydrate-lipid metabolism, which affected the processes of cell bioenergetics. These changes are most pronounced in the group of animals that were kept under conditions of constant darkness, which can serve as a marker of the initial stage of pathological desynchronosis. The jetlag-type model can be used to evaluate the effectiveness of the pharmacological correction of physiological desynchronosis. The model with modified light regimen can be used for evaluation of the effectiveness of pharmacological correction of pathological desynchronosis.
Asunto(s)
Ritmo Circadiano/fisiología , Oscuridad , Síndrome Jet Lag/sangre , Síndrome Jet Lag/fisiopatología , Alanina Transaminasa/metabolismo , Albúminas/metabolismo , Animales , Metabolismo de los Hidratos de Carbono , Creatinina/metabolismo , Hipotálamo/metabolismo , Luz , Metabolismo de los Lípidos , Masculino , Periodicidad , Ratas , Ratas Wistar , Programas Informáticos , Núcleo Supraquiasmático/metabolismo , Factores de TiempoRESUMEN
Since the discovery in 2001, the G protein-coupled trace amine-associated receptor 1 (TAAR1) has become an important focus of research targeted on evaluation of its role in the central nervous system (CNS). Meanwhile, impact of TAAR1 in the peripheral organs is less investigated. Expression of TAAR1 was demonstrated in different peripheral tissues: pancreatic ß-cells, stomach, intestines, white blood cells (WBC), and thyroid. However, the role of TAAR1 in regulation of hematological parameters has not been investigated yet. In this study, we performed analysis of anxiety-related behaviors, a complete blood count (CBC), erythrocyte fragility, as well as FT3/FT4 thyroid hormones levels in adult and middle-aged TAAR1 knockout mice. Complete blood count analysis was performed on a Siemens Advia 2120i hematology analyzer and included more than 35 measured and calculated parameters. Erythrocyte fragility test evaluated spherocytosis pathologies of red blood cells (RBC). No significant alterations in essentially all these parameters were found in mice without TAAR1. However, comparative aging analysis has revealed a decreased neutrophils level in the middle-aged TAAR1 knockout mouse group. Minimal alterations in these parameters observed in TAAR1 knockout mice suggest that future TAAR1-based therapies should exert little hematological effect and thus will likely have a good safety profile.
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Ansiedad/sangre , Receptores Acoplados a Proteínas G/sangre , Receptores Acoplados a Proteínas G/deficiencia , Factores de Edad , Animales , Ansiedad/psicología , Relación Dosis-Respuesta a Droga , Masculino , Ratones , Ratones de la Cepa 129 , Ratones Endogámicos C57BL , Ratones Noqueados , Cloruro de Sodio/toxicidadRESUMEN
We performed immunohistochemical analysis of the expression of caspases 3, 9 and bcl-2 protein in rat brain at various terms after administration of LD50 of sodium thiopental. Expression of the specified apoptosis markers was found in the sensorimotor cortex and hippocampus (dentate gyrus and CA2 region).
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Encéfalo/metabolismo , Caspasa 3/metabolismo , Caspasa 9/metabolismo , Coma/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Animales , Apoptosis/fisiología , Encéfalo/enzimología , Giro Dentado/metabolismo , Hipocampo/metabolismo , Inmunohistoquímica , Masculino , RatasRESUMEN
The efficacy of cytoflavin as a supporting drug in the CAF chemotherapy of breast cancer was studied. It was estimated by the tolerability, the peripheric blood index dynamics, the patients general condition (Karnovsky Index) and the frequency of the side effects. For the immediate estimation of the polychemotherapy and cytoflavin effects on the cytoprotection natural system state, the dynamics of the glutathione metabolism and the lipid peroxidation in the erythrocytes was investigated.
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Protocolos de Quimioterapia Combinada Antineoplásica , Neoplasias de la Mama/tratamiento farmacológico , Mononucleótido de Flavina/uso terapéutico , Inosina Difosfato/uso terapéutico , Niacinamida/uso terapéutico , Succinatos/uso terapéutico , Adulto , Anciano , Combinación de Medicamentos , Femenino , Mononucleótido de Flavina/efectos adversos , Humanos , Inosina Difosfato/efectos adversos , Persona de Mediana Edad , Niacinamida/efectos adversos , Calidad de Vida , Succinatos/efectos adversosRESUMEN
Experimental data on the influence of repeated administration of doxorubicin in a dose of 2.24 mg/kg on the glutathione exchange in heart tissues and erythrocytes of white outbred rats are presented. It was shown that disorders in the natural cytoprotection system caused by the introduction of this cardiotropic agent, can be corrected using a combined preparation of cytoflavin.
Asunto(s)
Cardiomiopatías/tratamiento farmacológico , Cardiotónicos/farmacología , Doxorrubicina , Mononucleótido de Flavina/farmacología , Inosina Difosfato/farmacología , Niacinamida/farmacología , Succinatos/farmacología , Animales , Cardiomiopatías/inducido químicamente , Cardiomiopatías/metabolismo , Cardiotónicos/uso terapéutico , Citoprotección , Combinación de Medicamentos , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Mononucleótido de Flavina/uso terapéutico , Glutatión/metabolismo , Inosina Difosfato/uso terapéutico , Peroxidación de Lípido/efectos de los fármacos , Masculino , Miocardio/metabolismo , Niacinamida/uso terapéutico , Ratas , Succinatos/uso terapéuticoRESUMEN
The experimental data concerning the influence of repeated administration of cyclophosphamide in a total dose of 200 mg/kg on the glutathione metabolism and lipid peroxidation in the liver and kidneys of albino noninbred rats are presented. The possibility of the natural cytoprotection system correction by the use of cycloflavin was shown. The cytoprotection action mechanisms of the pharmacological agent are discussed.