RESUMEN
It is established that ion exchange resins AV-17-2P, KU-2, AV-16-GS, AM 21A, IMAC-HP, PUROLITE and fiber VION KN-1 can be applied as carriers for inulinase immobilization. The analysis of IR spectra for an enzyme, carriers and heterogeneous enzyme preparations showed that inulinase binding to matrices of various carriers occurs in general through electrostatic interactions. It is assumed that the mechanisms of interaction between inulinase from Kluyveromyces marxianus and the matrices of cation- and anion exchange polymers differ essentially from each other: different sites of protein molecule take part in adsorption that causes various conformational reorganizations in an enzyme molecule.
Asunto(s)
Proteínas Fúngicas/química , Glicósido Hidrolasas/química , Resinas de Intercambio Iónico/química , Kluyveromyces/enzimología , Absorción FisicoquímicaRESUMEN
Method for express diagnosis of mechanical corneal injury using infrared spectroscopic analysis of tear fluid is proposed. Differential diagnosis of open and closed corneal injuries in absence of clinical data is presented. Clinical material is provided by patients with open globe injuries--25 patients (25 eyes) and 25 patients (25 eyes) with closed globe injuries. 20 healthy adults (40 eyes) were included into the control group. Proposed method allows to develop treatment strategy, determine extent of surgical interventions in corneal trauma and predict the course of posttraumatic process and complications as well.