Asunto(s)
Animales Recién Nacidos/fisiología , Neoplasias Experimentales/fisiopatología , Timo/fisiología , Factores de Edad , Animales , Femenino , Hibridación Genética , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos CBA , Ratones Endogámicos , Trasplante de Neoplasias , Trasplante HomólogoAsunto(s)
Animales Recién Nacidos/fisiología , Neoplasias Experimentales/fisiopatología , Animales , Supervivencia Celular , Femenino , Neoplasias Hepáticas Experimentales/fisiopatología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Trasplante de Neoplasias/métodos , Neoplasias Ováricas/fisiopatología , Efectos de la Radiación , Neoplasias del Recto/fisiopatología , Sarcoma Experimental/fisiopatología , Bazo/citología , Factores de TiempoRESUMEN
After a suspension of tumor pieces was grafted into newborn and adult (CBA X C57BL/6J)F1 and BALB/c mice, the growth of Lewis lung adenocarcinoma and mammary gland adenocarcinoma was inhibited in newborn mice, whereas sarcoma of the rectum (SR-1-75) grew at the same rate in newborn and adult recipients. Neonatal thymectomy stimulated the growth of hepatoma (H-2-73) in newborns. The degree of tumor growth inhibition was age-dependent: The maximum inhibition was observed in 1- to 6-day-old recipients, but later it gradually decreased. The hepatoma (H-2-73) and ovarian carcinoma (OC-1-75) with inhibited growth in newborns and the tumor (SR-1-75) with uninhibited growth had equally low immunogenicity. The data suggested that newborns possess factors that inhibit tumor growth but these factors disappear with increased age of recipients.
Asunto(s)
Inmunidad , Neoplasias Experimentales/inmunología , Timo/fisiología , Factores de Edad , Animales , Animales Recién Nacidos , Femenino , Rechazo de Injerto , Masculino , Ratones , Ratones Endogámicos , Trasplante de Neoplasias , Timectomía , Trasplante IsogénicoRESUMEN
Spleen cells from C57BL/6J or CBA mice inoculated iv with spleen cells from BALB/c mice produced a strong nonspecific cytotoxic effect on target cells (mouse L-cells). Lymph node cells from CBA or C57BL/6J mice inoculated sc with BALB/c spleen cells also destroyed L-cells. Lymph node cells from mice inoculated with syngeneic spleen cells were not cytotoxic. The cytotoxic effect was observed ion of allogeneic but not syngeneic spleen cells. This effect was considerably reduced or completely suppressed after partial or total removal of plastic-adherent cells.
Asunto(s)
Linfocitos/inmunología , Bazo/inmunología , Animales , Células Cultivadas , Pruebas Inmunológicas de Citotoxicidad , Femenino , Interferones/biosíntesis , Interferones/inmunología , Ganglios Linfáticos/inmunología , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Factores de Tiempo , Trasplante Homólogo , Trasplante IsogénicoRESUMEN
Growth of various fetal tissues and transplantable tumors in syngeneic newborn and adult mice [BALB/c, DBA/2, and (CBA X C57BL/6J)F1] was compared. Fetal skin, a mixture of all fetal tissues, and tumors were transplanted. The tumors arose spontaneously [hepatomas, mammary gland adenocarcinoma (MGAC)] or resulted from malignant conversion of ectopic transplants either of fetal tissues (urinary bladder carcinoma, adenocarcinoma of small intestine, stomach sarcoma) or of adult animal tissues (ovarian carcinoma) in the syngeneic system. The growth of fetal skin transplants and teratomas, which developed after transplantation of minced tissue from 18- to 20-day and 12- to 14-day fetuses, was considerably inferior in newborn syngeneic recipients, as compared with similar transplants in adults. Inhibition of tumor growth observed in newborn animals was manifested in prolongation of latent period before tumor node appearance and in slowing of growth rate of developed tumors. One of six tumors studied (MGAC) grew at the same rate in newborn and adult recipients. It was suggested that a special type of cellular and/or humoral mechanisms controlling tumor growth exists in newborns. The activity of such factors was conceivably based on fetal tumor antigens as targets. We assumed that weakly antigenic and strongly antigenic tumors behaved differently in respect to nonimmune and immune surveillance mechanisms.
Asunto(s)
Animales Recién Nacidos/inmunología , Feto/inmunología , Neoplasias Experimentales/inmunología , Inmunología del Trasplante , Factores de Edad , Animales , Carcinoma Hepatocelular/inmunología , Femenino , Neoplasias Hepáticas/patología , Masculino , Neoplasias Mamarias Experimentales/inmunología , Ratones , Ratones Endogámicos , Trasplante de Neoplasias , Neoplasias Ováricas/inmunología , Piel/embriología , Trasplante de Piel , Neoplasias Gástricas/inmunología , Teratoma/inmunología , Trasplante Isogénico , Neoplasias de la Vejiga Urinaria/inmunologíaRESUMEN
A comparative study of growth of a variety of fetal tissues and transplantable tumors in syngeneic newborn and adult mice was carried out. Tumors used in the experiments arose spontaneously (hepatomas, mammary gland adenocarcinoma) or resulted from malignant conversion of ectopic transplants either of fetal tissues (urinary bladder carcinoma, adenocarcinoma of small intestine, stomach sarcoma) or of adult animal tissues (ovary carcinoma) in syngeneic system. The growth of "teratomas" developed after transplantation of minced tissues of 18-20-day fetuses was considerably inferior in newborn syngeneic recipients as compared to analogous transplants in adults. Inhibition of tumor growth was also observed in newborn animals. It was manifested in prolongation of latent period before tumor node appearance as well as in slowing down of growth rate of developed tumors. Only one tumor, mammary gland adenocarcinoma, proved to be an exception, its growth being equally progressive in newborn and adult recipients. At transplantation of tumor cells mixed with lymphocytes of adult mouse spleen, stimulation of tumor growth in newborns and inhibition of growth in adult recipients was observed. It is suggested that there exists a special type of cellular or humoral mechanism controlling tumor growth in newborns. The activity of such factors is conceivably based on fetal antigens as targets.
Asunto(s)
Animales Recién Nacidos/inmunología , Anticuerpos Antineoplásicos , Feto/inmunología , Inmunidad Celular , Neoplasias Experimentales/inmunología , Adenocarcinoma/inmunología , Animales , Carcinoma/inmunología , Carcinoma Hepatocelular/inmunología , Femenino , Neoplasias Intestinales/inmunología , Neoplasias Hepáticas , Masculino , Neoplasias Mamarias Experimentales/inmunología , Ratones , Trasplante de Neoplasias , Neoplasias Ováricas/inmunología , Sarcoma Experimental/inmunología , Bazo/inmunología , Neoplasias Gástricas/inmunología , Neoplasias de la Vejiga Urinaria/inmunologíaAsunto(s)
Animales Recién Nacidos/inmunología , Trasplante de Neoplasias , Neoplasias Experimentales/inmunología , Adenocarcinoma/inmunología , Animales , Carcinoma Hepatocelular/inmunología , Femenino , Neoplasias Hepáticas/inmunología , Masculino , Neoplasias Mamarias Experimentales/inmunología , Ratones , Sarcoma Experimental/inmunología , Neoplasias Gástricas/inmunología , Factores de Tiempo , Neoplasias Urogenitales/inmunologíaRESUMEN
The injection of sera of adult mice or of a 20% extract of small and large intestine, liver, kidney, spleen, thymus, and lungs of adult mice suppressed the alpha1 fetoprotein production in syngeneic newborn recipients. Extracts of the striated muscles of adult mice had a meager inhibitory effect. Extracts of mouse embryonic intestine and those of the while embryo without the gastrointestinal tract had almost negligible amounts of suppressing factor. The possible role of humoral factors regulating the synthesis of embryonic antigens for the control of tumor growth was discussed author...