RESUMEN
The article deals with the actual problem of modern treatment of infant hemangiomas. The accumulated 10-year experience of using propranolol in 512 patients has shown its effectiveness and safety. The team of authors developed a protocol for managing patients with infantile hemangiomas of various localization at all stages of treatment. Special attention was paid to the cardiological aspect of patient monitoring using modern methods of instrumental diagnostics. Due to the clear application of the created protocol with the use of ultrasound control, in most cases, it was possible to achieve complete cure of this pathology, minimize incidence of undesirable effects and avoid the formation of relapses (rebound effect). The effectiveness of the developed algorithm of diagnosis and treatment is confirmed by both clinical results and the results of instrumental examinations.
Asunto(s)
Antagonistas Adrenérgicos beta , Hemangioma , Propranolol , Antagonistas Adrenérgicos beta/uso terapéutico , Algoritmos , Tratamiento Conservador , Hemangioma/tratamiento farmacológico , Humanos , Lactante , Propranolol/uso terapéutico , Resultado del TratamientoRESUMEN
Internasal administration of gamma-aminobutyric acid (GABA) induced prolonged behaviour changes and the appearance of three new compounds absent in the brain extracts of control rats. Two peptides associated with GABA administration were isolated and sequenced: Thr-Tyr-Thr-Phe, which corresponds to a gamma-immunoglobulin segment, and Val-Leu, which is present in a great number of proteins, hence its precursor could not be established. The third compound was not amenable to the Edman degradation technique. The data obtained show that the introduction of a neurotransmitter could cause specific changes in the levels of tissue-specific peptide components.
Asunto(s)
Encéfalo/metabolismo , Péptidos/química , Péptidos/metabolismo , Ácido gamma-Aminobutírico/metabolismo , Animales , Conducta Animal/efectos de los fármacos , Encéfalo/efectos de los fármacos , Cromatografía Líquida de Alta Presión/métodos , Péptidos/efectos de los fármacos , Ratas , Ácido gamma-Aminobutírico/farmacologíaRESUMEN
Fractionation of bovine brain extracts followed by automatic Edman sequencing of individual components resulted in identification of 107 endogenous peptides formed from functional proteins (haemoglobin, myelin basic protein, cytochrome c oxidase, etc) or unknown precursors. Several of the newly identified brain peptides demonstrate different types of biological activity; some of the substances show considerable overlap with the known biologically active peptides. It is suggested that these peptides should participate in regulation of extracellular and intracellular biochemical processes. A concept of 'tissue-specific peptide pool' is formulated describing a novel system of peptidergic regulation, complementary to the conventional hormonal and neuromodulatory systems. According to that description functional proteins provide their proteolytically derived fragments for maintaining the tissue homeostasis by modulating the availability of peptide receptors to respective 'true' ligands.
Asunto(s)
Química Encefálica , Péptidos/química , Secuencia de Aminoácidos , Animales , Bovinos , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Datos de Secuencia Molecular , Péptidos/aislamiento & purificación , Péptidos/metabolismo , Conformación ProteicaRESUMEN
Six short-chain peptides were isolated from an acidic extract of bovine brain in the course of total peptide screening. Their primary structures determined by Edman degradation were LVVYP, LVVYPWT, LVVYPWTQ, LVVYPWTQRF, VVYPWTQ and VVYPWTQRF, which respectively corresponded to the fragments 31-35, 31-37,31- 38, 31-40, 32-38 and 32-40 of bovine hemoglobin beta-chain. All these peptides contained sequences of opioid peptides - hemorphins. For two of these peptides, viz. 32-38 and 31-40, isolated from other sources, an opioid activity was demonstrated formerly.
Asunto(s)
Química Encefálica , Hemoglobinas/química , Oligopéptidos/química , Fragmentos de Péptidos/química , Secuencia de Aminoácidos , Animales , Bovinos , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Humanos , Datos de Secuencia Molecular , Oligopéptidos/aislamiento & purificación , Fragmentos de Péptidos/aislamiento & purificación , Homología de Secuencia de Aminoácido , PorcinosRESUMEN
Kinin-like properties of two new peptides NRP-11 and P7 which have structural similarity with neurotensin (NT) and kallidin (K) were investigated. It was found that, unlike NT and K, the new peptides possess reduced myotropic and hypotensive activity. On the other hand, similarly to NT and K, the new peptides exhibited a high histamine-releasing activity in rat peritoneal mast cells. Possible central effects are implied for peptide NRP-11 isolated from bovine brain and its fragment P7.