RESUMEN

In experiments on isolated neonatal cardiomyocytes and hearts of adult rats we investigated the influence of polyunsaturated fatty acids (PUFA) on expression of two peroxisome proliferator-activated receptor (PPAR) target genes--fatty acid transport protein (FATP) and interleukin-1 receptor antagonist (IL-1ra). We determined that cell membranes of adult rats heart and cardiomyocytes of neonatal rats which were born by females fed with omega-3 PUFA for 4 weeks, contain more omega-3 PUFA (alpha-linolenic and docosahexaenoic acids) in comparison with the control. It was shown that omega-3 PUFA elevated FATP and IL-1ra mRNA expression in 2.25 (P = 0.07) and 8.4 (P < 0.00001) folds correspondingly in adult rats tissues. In isolated cardiomyocytes, the mRNA expression of FATP and IL-1ra were augmented 2.5 folds (P = 0.22) and 8.1 folds (P < 0.00001), accordingly. Our data show that omega-3 PUFA stimulate PPAR target genes expression and allow to permit genetic mechanisms in cardioprotective effects of omega-3 PUFA.

Asunto(s)

Cardiotónicos/farmacología , Proteínas de Transporte de Ácidos Grasos/genética , Ácidos Grasos Omega-3/farmacología , Expresión Génica/efectos de los fármacos , Proteína Antagonista del Receptor de Interleucina 1/genética , Miocitos Cardíacos/efectos de los fármacos , Receptores Activados del Proliferador del Peroxisoma/genética , Animales , Animales Recién Nacidos , Femenino , Corazón/efectos de los fármacos , Corazón/embriología , Corazón/crecimiento & desarrollo , Masculino , Exposición Materna , Miocitos Cardíacos/metabolismo , Embarazo , Ratas , Ratas Wistar