RESUMEN
This paper presents the development of wrist ROM measurement system that was implemented on a rehabilitation glove for paretic hand, Easy-Flex, using a rotary potentiometer. This approach enables the device to measure the angles of up to 80° with the approximate resolution of 0.1°. The method along with a universal goniometer and a smartphone clinometer application were tested on 17 healthy participants in terms of reliability. The three instruments were able to accurately measure three different angles (flexion: -10°, neutral: 0°, and extension: 20°) with measurement errors less than 2°. Moreover, ICCs of the proposed method were close to 0.90 indicating the method implemented on Easy-Flex is reliable for measuring wrist flexion and extension measurement.
Asunto(s)
Paresia/rehabilitación , Rango del Movimiento Articular/fisiología , Rehabilitación/métodos , Muñeca/fisiopatología , Femenino , Humanos , Masculino , Diseño de Prótesis , Articulación de la Muñeca/fisiopatologíaRESUMEN
Trifluorovinyl groups are introduced onto the cyclopentadienyl ligands of ferrocene at the 1-, 1,1'-, and 1,2-positions by Negishi-type and Stille-type coupling reactions of trifluorovinylzinc chloride and tri-n-butyltrifluorovinyl stannane with several iodoferrocenes. Modification of the trifluorovinyl group by nucleophilic substitution and [2+2] cycloaddition make them versatile building blocks for synthetic transformations. 1,1'-Bis(trifluorovinyl) ferrocene reacts upon contact with silica or oxidizing agents and in the presence of a suitable nucleophile through a redox autocatalytic mechanism to afford ferrocenophanes with fluorinated handles. C(F)(H) and C(F)(OMe) moieties in α-positions allowed further modifications to be performed by nucleophilic substitution of the fluorine atoms. A series of ferrocenes with fluorinated side chains and ferrocenophanes with fluorinated handles were isolated and characterized. Several molecular structures were determined by single-crystal X-ray diffraction. The influence of the fluorine substituents on the redox properties of the iron center were studied by cyclic voltammetry.
RESUMEN
Carbon nanotubes (CNTs), promising novel nanomaterials, have been applied to drug delivery and bio-imaging; however, their potential harmful effects on human health and environment have gained much attention recently. In the present study, we investigated cytotoxic effect of solubilized single-walled CNTs (SWCNTs), which were dispersed in water by sodium dodesyl sulfate (SDS), in normal rat kidney epithelial cells (NRK-52E). SDS-SWCNT (0.125-10 µg/mL)-treated NRK-52E cells showed decreased cell viability and enhanced cytotoxicity marker levels following 24-48 h incubation. In addition, SDS-SWCNT treatment evoked the cell growth inhibition: 8 µg/mL SDS-SWCNT induced the growth arrest at G(0)/G(1) phase and levels of cell cycle-related proteins such as CDK2, CDK6 and phosphorylated-retinoblastoma (pRB) were significantly reduced by CNT. Whereas, at higher concentration of SDS-SWCNT, the percentage of cell numbers in apoptotic sub-G(1) phase was substantially increased. Along with these changes, SDS-SWCNT treatment elevated protein levels for p53 and p21 with a concomitant increase in the single strand DNA breakage. Taken together, these results suggest that SDS-solubilized SWCNTs exert genotoxic effect in renal epithelial cells, and p53-dependent signaling can be associated with the growth arrest and apoptosis events upon CNT-induced DNA damage.
Asunto(s)
Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Células Epiteliales/efectos de los fármacos , Riñón/efectos de los fármacos , Nanotubos de Carbono/toxicidad , Animales , Western Blotting , Técnicas de Cultivo de Célula , Ciclo Celular/efectos de los fármacos , Proteínas de Ciclo Celular/metabolismo , Línea Celular , Supervivencia Celular/efectos de los fármacos , Ensayo Cometa , Daño del ADN , Células Epiteliales/metabolismo , Células Epiteliales/patología , Riñón/metabolismo , Riñón/patología , Nanotubos de Carbono/química , Ratas , Proteína de Retinoblastoma/metabolismo , Dodecil Sulfato de Sodio/química , Solubilidad , Tensoactivos/químicaRESUMEN
Reaction of alkyne allyl alcohols tethered with N-(p-tolylsulfonamide) in the presence of a cationic gold(I) catalyst gave new cycloisomerization products, 4-oxa-6-azatricyclo[3.3.0.0(2,8)]octanes.
RESUMEN
The cycloisomerization reaction of N-allyl-2-(2'-arylethyne-2-yl)amides or allylic 2-alkynoates in the presence of cationic triphenylphosphinegold(I) produced bicyclo[3.2.0]hept-6-en-2-ones under mild conditions. Computational investigations with a density functional theory (DFT) revealed that a stepwise 6-endo-dig cyclization of 1,6-enyne systems, followed by a skeletal rearrangement to form bicyclo[3.2.0]hept-6-en-2-one species represents the most probable reaction pathway. This can be further supported by employing N-(1',1'-dimethyl-1-propen-3-yl)-2-(1''-phenylethyne-2''-yl)amide as a substrate, which exclusively produced 4-phenyl-5-(prop-1-en-2-yl)-5,6-dihydropyridino-2(1H)-one unambiguously via 6-endo-dig cyclization. Theoretical calculations suggest both an aryl group at the terminal alkyne and a keto carbonyl at the C(5) position of the 1,6-enyne system play a complementary role for the reactivity.
RESUMEN
Tying up loose ends: The reaction of bisallenes tethered with N-(p-tolylsulfonamide) in the presence of a cationic gold N-heterocyclic carbene catalyst gave new cycloisomerization products, 6,7-dimethyleneazabicyclo[3.1.1]heptanes, in high yields (see scheme; IPr = N,N'-bis(2,6-diisopropylphenyl)imidazol-2-ylidene).