RESUMEN
Peroxynitrite (ONOO-), a powerful biological oxidant, is produced in the mitochondria and reacts with many biomolecular targets under various pathological conditions, leading to a range of disease states. In this work, we developed a nanoliposome-encapsulated ratiometrically fluorescent probe (NRF) based on a hemicyanine structure Cy-O obtained by facile synthesis. Upon reaction with ONOO-, the oxidation and hydrolysis of a π-conjugation system within the nanoliposome triggers a ratiometrically fluorescent response and a large-scale emission shift (238 nm), which provides a specific and sensitive means for the ONOO- detection. Moreover, we have performed DFT calculation at the 6-31+G(d,p) level using a suite of Gaussian 09 programs to obtain insights into the chemical structure optical properties of Cy-O. In addition, the practical applications of the nanoprobe to image exogenous and endogenous ONOO- were achieved further in live cells and animals triumphantly.
Asunto(s)
Materiales Biocompatibles/química , Colorantes Fluorescentes/química , Nanopartículas/química , Ácido Peroxinitroso/análisis , Animales , Materiales Biocompatibles/síntesis química , Teoría Funcional de la Densidad , Colorantes Fluorescentes/síntesis química , Células Hep G2 , Humanos , Liposomas/química , Neoplasias Hepáticas Experimentales/diagnóstico por imagen , Ensayo de Materiales , Ratones , Estructura Molecular , Imagen Óptica , Tamaño de la PartículaRESUMEN
A new probe RB-CR for the detection of Cr3+ was constructed based on the conjugation between rhodamine B and phenylthiourea. The Cr3+ could trigger opening of the rhodamine spirolactam of RB-CR upon complexation selectively, sensitively and rapidly, resulting in a pronounced enhancement of absorption and fluorescence signal. Further fluorescence imaging study has suggested that RB-CR could be applied as a probe for Cr3+ surveillance in living HepG2 cells with low cytotoxicity, which provide us the feasibility of exploring the Cr3+ recognition process by the turn-on fluorescence response.
Asunto(s)
Colorantes Fluorescentes , Imagen Óptica , Células HeLa , Humanos , Rodaminas , Espectrometría de FluorescenciaRESUMEN
A red fluorescent probe (Mito-V) with a long lifetime was designed to monitor viscosity changes with high selectivity and sensitivity. The fluorescence intensity and lifetime of Mito-V displayed a good relationship with the viscosity value, and Mito-V was successfully applied to sensing mitochondrial viscosity changes in living cells under different biological processes.