RESUMEN
By an indirect immunohistochemical method with fluorescein-isothiocyanate (FITC) and horse radish peroxidase as markers (HRP) the presence of vasopressin was shown in cells of dura mater in white rats. Mast cells were identified after staining with methylene blue by the metachromatic granularity of the cytoplasm. It was shown that the number of cells found by means of FITC luminescence corresponds with their number found by means of methylene blue. The use of conjugate with HRP unveils a lesser number of vasopressin-containing cells.
Asunto(s)
Duramadre/análisis , Vasopresinas/análisis , Animales , Duramadre/citología , Fluoresceína-5-Isotiocianato , Fluoresceínas , Peroxidasa de Rábano Silvestre , Inmunohistoquímica , Mediciones Luminiscentes , Mastocitos/análisis , Azul de Metileno , Ratas , TiocianatosRESUMEN
Immunological responsiveness of two lines of inbred mice, BALb/c and CBA, was studied by immunization of the animals with concentrated tick-borne encephalitis vaccine, and the role of cell-mediated immunity in formation of resistance of the two genetic lines of mice was evaluated. Determinations of the minimal immunizing dose of the vaccine revealed differences in parameters of the immunizing effect. Studies of the effect of TBE vaccine on the status of cell-mediated immunity by lymphocyte blastogenesis test led to a conclusion that vaccination of BALb/c mice contributed to the realization of the mitogenic effect of the antigen used to much greater extent.
Asunto(s)
Virus de la Encefalitis Transmitidos por Garrapatas/patogenicidad , Ratones Endogámicos BALB C/inmunología , Ratones Endogámicos CBA/inmunología , Animales , Susceptibilidad a Enfermedades , Relación Dosis-Respuesta Inmunológica , Virus de la Encefalitis Transmitidos por Garrapatas/inmunología , Inmunidad Celular , Inmunización , Linfocitos/inmunología , Ratones , Vacunas Virales/inmunologíaRESUMEN
The sensitivity of different lines of inbred mice to strains of the tick-borne encephalitis complex viruses differing in their biological properties and the degree of attenuation was compared. The mice differing in their genetic characteristics and susceptibility to extraneurally inoculated virus were shown to have different immunological responsiveness to inoculation of an inactivated vaccine antigen.