RESUMEN
Myofibrillar protein tropomyosin (TM) is a normal physiological protein participating in regulation of muscular contraction. It is widely prevalent among living organisms. This explains cross-reactivity of allergic patients to home dust, sea fish, cockroaches, etc. The presence of similar IgE-binding epitopes in TM of different origin is a key factor in development of cross-reactivity (CR). CR to TM is a general biological phenomenon. We consider modified TM as a basic component in design of allergovaccines of a new generation.
Asunto(s)
Alérgenos/inmunología , Artrópodos/metabolismo , Reacciones Cruzadas/inmunología , Hipersensibilidad/inmunología , Tropomiosina/inmunología , Alérgenos/administración & dosificación , Alérgenos/efectos adversos , Alérgenos/biosíntesis , Animales , Artrópodos/inmunología , Desensibilización Inmunológica , Humanos , Hipersensibilidad/prevención & control , Contracción Muscular/fisiología , Miofibrillas/inmunología , Miofibrillas/metabolismo , Miofibrillas/fisiología , Proteínas Recombinantes/administración & dosificación , Proteínas Recombinantes/efectos adversos , Proteínas Recombinantes/inmunología , Tropomiosina/administración & dosificación , Tropomiosina/efectos adversos , Tropomiosina/biosíntesisAsunto(s)
Anafilaxia/diagnóstico , Venenos de Artrópodos/efectos adversos , Hipersensibilidad a las Drogas/diagnóstico , Vías Férreas , Hipersensibilidad Respiratoria/diagnóstico , Adolescente , Adulto , Factores de Edad , Anciano , Anafilaxia/epidemiología , Anafilaxia/etiología , Niño , Hipersensibilidad a las Drogas/epidemiología , Hipersensibilidad a las Drogas/etiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Hipersensibilidad Respiratoria/epidemiología , Hipersensibilidad Respiratoria/etiología , Factores Sexuales , Siberia/epidemiología , Encuestas y CuestionariosRESUMEN
Research on isolated hearts of Rana temporaria has shown that upon treating them with anti-cholinoreceptor antibodies, there occurs a considerable reduction of inhibition effect on cardiac activity of acetylcholine or carbacholine. A reduction of inhibition effect was noticed after incubation of frog's heart with antibodies against cholinoreceptors obtained from motor-denervated muscles of frogs as well as from muscles of mice Balb/c. Cholinoreceptor protein was obtained and purified by A. Sobel's method. Control tests were made with serum of non-immunized rabbits and rabbits immunized with material obtained from non-denervated muscles of frogs. It was concluded that acetylcholinoreceptor antibodies are capable of provoking atropine-like effect on frog's heart. According to our data, anti-cholinoreceptor antibodies as well as cholinoreceptors are relatively non-specific to species.
Asunto(s)
Anticuerpos/inmunología , Miocardio/inmunología , Receptores Colinérgicos/inmunología , Acetilcolina/farmacología , Animales , Carbacol/farmacología , Femenino , Corazón/efectos de los fármacos , Técnicas In Vitro , Masculino , Ratones , Ratones Endogámicos BALB C , Contracción Miocárdica , Conejos , Rana temporariaRESUMEN
The influence of anti-cholinoceptor antibodies on the process of immune rosette-formation in BALBc mice was studied.
Asunto(s)
Sueros Inmunes/farmacología , Receptores Colinérgicos/inmunología , Formación de Roseta , Animales , Ratones , Ratones Endogámicos BALB CRESUMEN
A study was made of changes in the balance of neuromediator monoamines and metabolistes in rat brain, induced by a single administration of pyrazidol, imipramine and norzimelidine. It was revealed that pyrazidal in a dose of 50 mg/kg increased the level of noradrenaline (NA) and serotonin (5-HT), particularly in the hypothalamus and raphe-nucleus of rat brain. In the same brain regions, imipramine (30 mg/kg) raised the NA content and lowered the concentration of 5-HT and dopamine (DA) metabolites. Norzimelidine (60 mg/kg) elevated the levels of NA and DA in the hypothalamus and reduced the 5-HT content in the raphe nucleus of rat brain. It is suggested that the topography of changes in the balance of biogenic amines in the brain induced by different antidepressants reflects the pharmacological features of their action.
Asunto(s)
Antidepresivos/farmacología , Encéfalo/metabolismo , Catecolaminas/metabolismo , Ácido 3,4-Dihidroxifenilacético/metabolismo , Animales , Carbazoles/farmacología , Corteza Cerebral/metabolismo , Interacciones Farmacológicas , Ácido Homovanílico/metabolismo , Ácido Hidroxiindolacético/metabolismo , Hipotálamo/metabolismo , Imipramina/farmacología , Masculino , Núcleos del Rafe/metabolismo , Ratas , Zimeldina/análogos & derivados , Zimeldina/farmacologíaRESUMEN
A study was made of the central effects of tuftsin (Thr-Lys-Pro-Arg) and its analogs (Leu1-tuftsin, D-Arg4-tuftsin) on the dopamine-dependent behavior and tyrosine hydroxylase (TH) activity. It was shown that the absence of direct effect of tuftsin and Leu1-tuftsin on postsynaptic dopaminergic receptors, revealed in experimental rotational behavior, correlates with a decrease in TH activity in the rat hypothalamus and striatum. Depression of the rotational behavior and increased activity of TH under the effect of D-Arg4-tuftsin suggest that this analog can modulate postsynaptic dopaminergic receptors by the antagonism type.
Asunto(s)
Fragmentos de Inmunoglobulinas/farmacología , Neurotransmisores/metabolismo , Psicotrópicos/farmacología , Tuftsina/farmacología , Animales , Conducta Animal/efectos de los fármacos , Masculino , Ratas , Ratas Endogámicas , Receptores Dopaminérgicos/efectos de los fármacos , Rotación , Tuftsina/análogos & derivadosRESUMEN
Adaptogenic effect of mebikar was revealed in experiments on cats, mice and rats. The drug was shown to decrease the emotional stress and to completely remove the stupor-like state in cats induced by stress situation and pain stimulation. The drug slightly increased the lifespan of animals under hypoxic hypoxia and raised the limits of maximum tolerable physical load. It lowered the accumulation of hypoxic lactate. Electron microscopy and biochemical analysis revealed the mebikar-induced increase in glycogen content in muscle cells of the myocardium and in skeletal muscles. The data obtained indicate that mebikar is conducive to optimization of the behavior and some processes of energy metabolism in animals exposed to emotional stress, exercise and hypoxic hypoxia.
Asunto(s)
Adaptación Fisiológica/efectos de los fármacos , Biureas/farmacología , Compuestos Bicíclicos con Puentes/farmacología , Hidrocarburos Aromáticos con Puentes/farmacología , Tranquilizantes/farmacología , Adaptación Psicológica/efectos de los fármacos , Animales , Gatos , Humanos , Hipoxia/tratamiento farmacológico , Masculino , Ratones , Esfuerzo Físico/efectos de los fármacos , Ratas , Estrés Psicológico/tratamiento farmacológicoRESUMEN
Effects of psychotropic drugs on emotional reactivity and behavior under acute stress were studied in rats with 6-hydroxydopamine (6-HDA)-destroyed catecholaminergic brain terminals. The differences in emotional and behavioral reactivity were found in the group of animals who received 6-HDA. An abrupt reduction of tyrosine hydroxylase activity in corpus striatum of "emotional" rats correlates with noticeable difficulties in the behavior of escape out of stress and essential differences in the effects of psychotropic drugs whose action is mediated via the catecholamine neurotransmitter system.
Asunto(s)
Ansiolíticos , Benzodiazepinas , Encéfalo/fisiopatología , Catecolaminas/fisiología , Neurotransmisores/fisiología , Psicotrópicos/uso terapéutico , Estrés Psicológico/tratamiento farmacológico , Anfetamina/uso terapéutico , Animales , Benzodiazepinonas/uso terapéutico , Clonidina/uso terapéutico , Haloperidol/uso terapéutico , Humanos , Ratas , Ratas Endogámicas , Estrés Psicológico/fisiopatologíaRESUMEN
The relationship between behavior manifestations, somatic alterations and brain tyrosine hydroxylase activity was studied in "emotional" and in "non-emotional" rats exposed to chronic stress. The drastic increase in tyrosine hydroxylase activity in the hypothalamus and striatum after stress correlates with disturbances in behavior, somatic defects (ulcers, erosions) in "emotional" rats as compared with "non-emotional" ones.
Asunto(s)
Conducta Animal , Encéfalo/enzimología , Emociones , Estrés Psicológico/diagnóstico , Tirosina 3-Monooxigenasa/metabolismo , Animales , Cuerpo Estriado/enzimología , Humanos , Hipotálamo/enzimología , Masculino , Actividad Motora , Ratas , Tiempo de Reacción , Úlcera Gástrica/etiología , Estrés Psicológico/complicaciones , Estrés Psicológico/enzimologíaRESUMEN
The experiments on rats exposed to compulsory swimming have shown the normalizing effect of sodium hydroxybutyrate with respect to one of the biochemical indices of physical overwork: the ammonia level in cross-striated muscles. In control rats who had not received the drug, the swimming induced a significant (more than twofold) increase in the muscle tissue ammonia level, whereas animals preventively treated with sodium hydroxybutyrate (single administration or two-week course) demonstrated no ammonia accumulation. It is suggested that sodium hydroxybutyrate preventing the toxic effect of one of the end products of nitrogen metabolism may reduce afteraction of muscular overwork.