RESUMEN

The involvement of skeletal muscle in the process of palatal development in mammals is an example of Waddingtonian epigenetics. Our earlier study showed that the cleft palate develops in the complete absence of skeletal musculature during embryonic development in mice. This contrasts with previous beliefs that tongue obstruction prevents the elevation and fusion of the palatal shelves. We argue that the complete absence of mechanical stimuli from the adjacent muscle, i.e., the lack of both static and dynamic loading, results in disordered palatogenesis. We further suggest that proper fusion of the palatal shelves depends not only on mechanical but also on paracrine contributions from the muscle. The muscle's paracrine role in the process of palatal fusion is achieved through its being a source of certain secreted and/or circulatory proteins. A cDNA microarray analysis revealed differentially expressed genes in the cleft palate of amyogenic mouse fetuses and suggested candidate molecules with a novel function in palatogenesis (e.g., Tgfbr2, Bmp7, Trim71, E2f5, Ddx5, Gfap, Sema3f). In particular, we report on Gdf11 mutant mouse that has cleft palate, and on several genes whose distribution is normally restricted to the muscle (completely absent in our amyogenic mouse model), but which are found down-regulated in amyogenic mouse cleft palate. These molecules probably present a subset of paracrine cues that influence palatogenesis from the adjacent muscle. Future studies will elucidate the role of these genes in muscle-palate crosstalk, connecting the cues produced by the muscle with the cartilage and bone tissue's responses to these cues, through various degrees of cell proliferation, death, differentiation and tissue fusion.

Asunto(s)

Músculo Esquelético/embriología , Hueso Paladar/embriología , Animales , Desarrollo Fetal , Ratones

RESUMEN

We study the role of muscle in the epigenetic (N.B., we use this term with the broader and more integrative meaning) shaping of developing motor neuron fate choices employing an approach based on mouse mutagenesis and pathology. The developmental role of skeletal muscle is studied in the whole mouse embryo by knocking out myogenic regulatory factors Myf5 and MyoD, to obtain an embryo without any skeletal musculature (Rudnicki et al., 1993). Our goal is to find muscle-provided trigger(s) of motor neuron death relevant to motor neuron diseases such as amyotrophic lateral sclerosis. The reason for this kind of thinking is the fact that a complete absence of lower and upper motor neurons, which is the pathological definition of amyotrophic lateral sclerosis, is only achieved in the complete absence of the muscle (Kablar and Rudnicki, 1999). Mutual embryonic inductive interactions between different tissue types and organs, between individual cell types belonging to the same or different lineages, and between various kinds of molecular players, are only some examples of the complex machinery that operates to connect genotype and phenotype. So far, our studies indicate that some aspects of this interplay can indeed be studied as proposed in this review article, suggesting the role of skeletal muscle in the epigenetic shaping of motor neuron fate choices. We will therefore continue this investigation as outlined to gain more insight into the nature of the epigenetic events that lead to the emergent properties of a phenotype.

Asunto(s)

Neuronas Motoras/metabolismo , Músculo Esquelético/metabolismo , Animales , Embrión de Mamíferos , Ratones , Neuronas Motoras/fisiología , Músculo Esquelético/embriología , Factores Reguladores Miogénicos/genética , Factores Reguladores Miogénicos/metabolismo

RESUMEN

Fetal breathing-like movements (FBMs) are important in normal lung growth and pneumocyte differentiation. In amyogenic mouse embryos (designated as Myf5-/-:MyoD-/-, entirely lacking skeletal musculature and FBMs), type II pneumocytes fail to differentiate into type I pneumocytes, the cells responsible for gas exchange, and the fetuses die from asphyxia at birth. Using oligonucleotide microarrays, we compared gene expression in the lungs of Myf5-/-:MyoD-/- embryos to that in normal lungs at term. Nine genes were found to be up-regulated and 54 down-regulated at least 2-fold in the lungs of double-mutant embryos. Since many down-regulated genes are involved in lymphocyte function, immunohistochemistry was employed to study T- and B-cell maturity in the thymus and spleen. Our findings of normal lymphocyte maturity implied that the down-regulation was specific to the double-mutant lung phenotype and not to its immune system. Immunostaining also revealed altered distribution of transcription and growth factors (SATB1, c-Myb, CTGF) from down-regulated genes whose knockouts are now known to undergo embryonic or neonatal death secondary to respiratory failure. Together, it appears that microarray analysis has identified a profile of genes potentially involved in pneumocyte differentiation and therefore in the mechanisms that may be implicated in the mechanochemical signal transduction pathways underlying FBMs-dependent pulmonary hypoplasia.

Asunto(s)

Diferenciación Celular , Regulación del Desarrollo de la Expresión Génica , Enfermedades Pulmonares/patología , Pulmón/patología , Proteína MioD/metabolismo , Factor 5 Regulador Miogénico/deficiencia , Análisis de Secuencia por Matrices de Oligonucleótidos , Animales , Diferenciación Celular/genética , Factor de Crecimiento del Tejido Conjuntivo , Perfilación de la Expresión Génica , Proteínas Inmediatas-Precoces/metabolismo , Péptidos y Proteínas de Señalización Intercelular/metabolismo , Pulmón/embriología , Pulmón/metabolismo , Pulmón/fisiopatología , Enfermedades Pulmonares/embriología , Enfermedades Pulmonares/genética , Enfermedades Pulmonares/fisiopatología , Linfocitos/metabolismo , Linfocitos/patología , Proteínas de Unión a la Región de Fijación a la Matriz/metabolismo , Ratones , Ratones Noqueados , Proteína MioD/genética , Factor 5 Regulador Miogénico/genética , Proteínas Proto-Oncogénicas c-myb/metabolismo , ARN Mensajero/metabolismo , Reproducibilidad de los Resultados , Mecánica Respiratoria , Músculos Respiratorios/embriología , Factores de Tiempo

RESUMEN

In this report we employed double-knock-out mouse embryos and fetuses (designated as Myf5-/-: MyoD-/- that completely lacked striated musculature to study bone development in the absence of mechanical stimuli from the musculature and to distinguish between the effects that static loading and weight-bearing exhibit on embryonic development of skeletal system. We concentrated on development of the mandibles (= dentary) and clavicles because their formation is characterized by intramembranous and endochondral ossification via formation of secondary cartilage that is dependent on mechanical stimuli from the adjacent musculature. We employed morphometry and morphology at different embryonic stages and compared bone development in double-mutant and control embryos and fetuses. Our findings can be summarized as follows: a) the examined mutant bones had significantly altered shape and size that we described morphometrically, b) the effects of muscle absence varied depending on the bone (clavicles being more dependent than mandibles) and even within the same bone (e.g., the mandible), and c) we further supported the notion that, from the evolutionary point of view, mammalian clavicles arise under different influences from those that initiate the furcula (wishbone) in birds. Together, our data show that the development of secondary cartilage, and in turn the development of the final shape and size of the bones, is strongly influenced by mechanical cues from the skeletal musculature.

Asunto(s)

Desarrollo Óseo , Clavícula/embriología , Biología Evolutiva/métodos , Regulación del Desarrollo de la Expresión Génica , Mandíbula/embriología , Proteína MioD/genética , Factor 5 Regulador Miogénico/genética , Animales , Evolución Biológica , Genotipo , Ratones , Ratones Noqueados , Ratones Transgénicos , Proteína MioD/fisiología , Factor 5 Regulador Miogénico/fisiología , Fenotipo , Factores de Tiempo

RESUMEN

As developmental biologists we study the role of fetal movements in providing continuity between prenatal and postnatal life. There are two major categories of fetal motility. The first category consists of movements that have an obvious effect on the survival or development of the fetus (e.g., changes of position, sucking and swallowing). The second category consists of fetal movements that anticipate postnatal functions. For example, fetal ocular movements (FOMs) predict postnatal eye function (e.g., motion vision) of the newborn and therefore represent an important indicator of fetal health. However, while the clinical significance of fetal motility is obvious, its biological significance is elusive. We propose to use retina of genetically modified mouse embryos to study the biological role of FOMs in the genesis of cell diversity and organ functional maturation. Our results have already demonstrated the importance of fetal eye motility in the differentiation of cholinergic amacrine cells (CACs) in the retina (Kablar, 2003). Apparently, these cells are sensitive to motion and also responsible for motion vision. In the current report, we suggest employing the unique opportunity provided by the mouse Myf5-/-:MyoD-/- knock-outs that lack skeletal musculature and FOMs, microarray analysis and the follow-up experiments to identify a group of candidate genes that are essential for the molecular regulation of CAC differentiation and in turn for the functional maturation of the visual system towards its ability to perform motion vision. Finally, the molecules identified via this approach may be important in the mechanochemical signal transduction pathways employed during the process of conversion of a mechanical stimulus into an instruction understandable by the developing retinal neurons and glia cells.

Asunto(s)

Diferenciación Celular , Movimientos Oculares/fisiología , Movimiento Fetal/fisiología , Retina/citología , Animales , Embrión de Mamíferos , Perfilación de la Expresión Génica , Ratones , Organogénesis

RESUMEN

Central nervous system and skeletal muscles secrete a group of polypeptide hormones called neurotrophins (NTs). More recent studies show that NTs and their receptors are also expressed in the lung, suggesting a role for NTs in lung development. To examine the role of NTs during normal and diseased lung organogenesis, we employed wild-type and amyogenic mouse embryos (designated as Myf5-/-:MyoD-/-). Amyogenic embryos completely lacked skeletal muscles and were not viable after birth due to the respiratory failure secondary to lung hypoplasia. To examine the importance of lung-secreted NTs during normal and hypoplastic lung organogenesis, immunohistochemistry was employed. Distribution of NTs and their receptors was indistinguishable between normal and hypoplastic lungs. To further examine the importance of non-lung-secreted NTs (e.g., from the skeletal muscle and CNS) in lung organogenesis, in utero injections of two NTs were performed. The exogenously introduced NTs (i.e., non-lung-secreted) did not appear to improve development of the lung in amyogenic embryos. Moreover, immunohistochemistry showed significantly reduced number of airway smooth muscle cells (ASMCs) in hypoplastic lungs of amyogenic embryos, suggesting that the number of ASMCs is primarily regulated by the fetal breathing-like movements (i.e., mechanical factors).

Asunto(s)

Embrión de Mamíferos/citología , Pulmón/patología , Factores de Crecimiento Nervioso/metabolismo , Animales , Regulación de la Expresión Génica , Inmunohistoquímica , Etiquetado Corte-Fin in Situ , Pulmón/embriología , Ratones , Ratones Transgénicos , Movimiento , Proteína MioD/metabolismo , Miocitos del Músculo Liso/citología , Factor 5 Regulador Miogénico/metabolismo , Respiración

RESUMEN

In this review the recent findings concerning the role of fetal breathing-like movements (FBMs) on lung organogenesis are discussed. We first review the consequences that the lack of FBMs has on lung organogenesis and then we discuss the possible pathways that may be employed in this process. Specifically, we review the data in support of the notion that FBMs are required for the cell cycle kinetics regulation (i.e., cell proliferation and cell death) via the expression of growth factors, such as platelet derived growth factors (PDGFs) and insulin growth factors (IGFs), and thyroid transcription factor 1 (TTF-1). Moreover, the role of FBMs on biochemical differentiation of Clara cells, type I and type II pneumocytes is reviewed. Interestingly, even though type II pneumocytes are able to synthesize surfactant-associated proteins (SPs), in the complete absence of FBMs, they are unable to compile, store and release the surfactant. Similarly, in spite of the expression of some early differentiation markers, in the absence of FBMs, type I pneumocytes are unable to flatten in order to allow the gas exchange in the lung. In fact, we are currently employing the cDNA microarray analysis in search for the molecules that might be specific for the lacking functions in pneumocytes.

Asunto(s)

Movimiento Fetal/fisiología , Pulmón/embriología , Pulmón/fisiología , Respiración , Animales , Humanos , Pulmón/crecimiento & desarrollo

RESUMEN

We report the cloning and initial characterization of a novel gene encoding the Disco interacting protein 2 (Dip2). dip2 DNA complementary to RNA (cDNA) showed a high degree of sequence similarity to cDNAs of unknown function previously identified in humans and Caenorhabditis elegans. We have cloned the mouse homolog of the dip2 cDNA and characterized the expression of this gene by Northern blotting analysis and in situ hybridization to whole mount embryos. Our observations demonstrate that there is a remarkable degree of sequence conservation at the dip2 locus that is reflected in the nervous system-specific expression of both the Drosophila and mouse homologs.

Asunto(s)

Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Proteínas del Tejido Nervioso/genética , Secuencia de Aminoácidos , Animales , Northern Blotting , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Mapeo Cromosómico , Clonación Molecular , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Drosophila melanogaster/embriología , Embrión de Mamíferos/metabolismo , Embrión no Mamífero/metabolismo , Exones , Regulación del Desarrollo de la Expresión Génica , Genes/genética , Hibridación in Situ , Intrones , Ratones , Datos de Secuencia Molecular , Unión Proteica , ARN Mensajero/genética , ARN Mensajero/metabolismo , Homología de Secuencia de Aminoácido , Factores de Transcripción/genética , Factores de Transcripción/metabolismo

RESUMEN

In this review we discuss the recent findings concerning the mechanisms that restrict somitic cells to the skeletal muscle fate, the myogenic regulatory factors controlling skeletal muscle differentiation and specification of myogenic cell lineages, the nature of inductive signals and the role of secreted proteins in embryonic patterning of the myotome. More specifically, we review data which strongly support the hypothesis that Myf-5 plays a unique role in development of epaxial muscle, that MyoD plays a unique role in development of hypaxial muscles derived from migratory myogenic precursor cells, and that both genes are responsible for development of intercostal and abdominal muscles (hypaxial muscles that develop from the dermatomal epithelia). In addition, while discussing upstream and post-translational regulation of myogenic regulatory factors (MRFs), we suggest that correct formation of the myotome requires a complex cooperation of DNA binding proteins and cofactors, as well as inhibitory function of non-muscle cells of the forming somite, whose proteins would sequester and suppress the transcription of MRFs. Moreover, in the third part of our review, we discuss embryonic structures, secreted proteins and myogenic induction. However, although different signaling molecules with activity in the process of somite patterning have been identified, not many of them are found to be necessary during in vivo embryonic development. To understand their functions, generation of multiple mutants or conditional/tissue-specific mutants will be necessary.

Asunto(s)

Músculo Esquelético/embriología , Animales , Linaje de la Célula , Desarrollo Embrionario y Fetal , Esbozos de los Miembros , Ratones , Músculo Esquelético/metabolismo , Factores Reguladores Miogénicos/metabolismo , Biosíntesis de Proteínas , Somitos

RESUMEN

Previously, coexpression of smooth and skeletal differentiation markers, but not myogenic regulatory factors (MRFs), was observed from E16.5 mouse fetuses in a small percentage of diaphragm level esophageal muscle cells, suggesting that MRFs are not involved in the process of initiation of developmentally programmed transdifferentiation in the esophagus. To investigate smooth-to-skeletal esophageal muscle transition, we analyzed Myf5nlacZ knock-in mice, MyoD-lacZ and myogenin-lacZ transgenic embryos with a panel of the antibodies reactive with myogenic regulatory factors (MRFs) and smooth and skeletal muscle markers. We observed that lacZ-expressing myogenic precursors were not detected in the esophagus before E15.5, arguing against the hypothesis that muscle precursor cells populate the esophagus at an earlier stage of development. Rather, the expression of the MRFs initiated in smooth muscle cells in the upper esophagus of E15.5 mouse embryos and was immediately followed by the expression of skeletal muscle markers. Moreover, transdifferentiation was markedly delayed or absent only in the absence of Myf5, suggesting that appropriate initiation and progression of smooth-to-skeletal muscle transdifferentiation is Myf5-dependent. Accordingly, the esophagus of Myf5(-/-):MyoD(-/-)embryos completely failed to undergo skeletal myogenesis and consisted entirely of smooth muscle. Lastly, extensive proliferation of muscularis precursor cells, without programmed cell death, occurred concomitantly with esophageal smooth-to-skeletal muscle transdifferentiation. Taken together, these results indicate that transdifferentiation is the fate of all smooth muscle cells in the upper esophagus and is normally initiated by Myf5.

Asunto(s)

Proteínas de Unión al ADN , Esófago/embriología , Proteínas Musculares/metabolismo , Músculo Esquelético/citología , Músculo Liso/citología , Proteína MioD/metabolismo , Transactivadores , Animales , Diferenciación Celular , División Celular , Ratones , Ratones Transgénicos , Proteínas Musculares/genética , Músculo Esquelético/embriología , Músculo Liso/embriología , Proteína MioD/genética , Factor 5 Regulador Miogénico , Miogenina/genética

RESUMEN

In this report the organizer activity of follistatin was examined by transplantation of pieces of the animal cap, isolated from embryos injected with follistatin mRNA, into the blastocoele of an early host blastula (Einsteck explants). Host embryos developed a secondary axis consisting of myotomes, notochord and neural tube of the trunk or tail character. Secondary structures that are characteristic of a head, such as cement glands or brain and eyes, did not develop in these experiments. These findings suggested that follistatin may have the trunk and tail organizer activity, while it was not possible to reconstitute its head organizer activity.

Asunto(s)

Blastocisto/efectos de los fármacos , Glicoproteínas/farmacología , Sustancias de Crecimiento/farmacología , Cabeza/embriología , Cola (estructura animal)/embriología , Tórax/embriología , Animales , Folistatina , Inmunohistoquímica , Xenopus laevis

RESUMEN

Mice lacking the transcription factors Myf-5 and MyoD lack all skeletal muscle and therefore present a unique opportunity to investigate the dependence of nervous system development on myogenesis. Motor neurons arose normally in the spinal cord of mutant embryos and by birth all somatic motor neurons were eliminated by apoptosis. By contrast, interneurons were not affected. Proprioceptive sensory neurons in the dorsal root ganglia underwent apoptosis. The facial motor nucleus was ablated of motor neurons and contained large numbers of apoptotic bodies. Surprisingly, giant pyramidal neurons were absent in the motor cortex without any corresponding evidence of apoptosis. The epaxial and cutaneous component of dorsal ramus failed to form in the absence of the myotome. Therefore, we conclude that nervous development is more intimately coupled to skeletal myogenesis than has previously been understood.

Asunto(s)

Encéfalo/embriología , Proteínas de Unión al ADN , Neuronas Motoras/patología , Proteínas Musculares/genética , Músculo Esquelético/embriología , Proteína MioD/genética , Médula Espinal/embriología , Transactivadores , Animales , Apoptosis , Embrión de Mamíferos/patología , Desarrollo Embrionario y Fetal , Ratones , Ratones Noqueados , Corteza Motora/patología , Desarrollo de Músculos , Proteínas Musculares/deficiencia , Músculo Esquelético/crecimiento & desarrollo , Mutación , Factor 5 Regulador Miogénico

RESUMEN

Gene targeting has indicated that the bHLH transcription factors Myf-5 and MyoD are required for myogenic determination because skeletal myoblasts and myofibers are entirely ablated in mouse embryos lacking both Myf-5 and MyoD. Entrance into the skeletal myogenic program during development occurs following the independent transcriptional induction of either Myf-5 or MyoD. To identify sequences required for the de novo induction of MyoD transcription during development, we investigated the expression patterns of MyoD-lacZ transgenes in embryos deficient in both Myf-5 and MyoD. We observed that a 258-bp fragment containing the core of the -20-kb MyoD enhancer activated expression in newly formed somites and limb buds in compound mutant embryos lacking both Myf-5 and MyoD. Importantly, Myf-5- and MyoD-deficient presumptive muscle precursor cells expressing beta-galactosidase were observed to assume nonmuscle fates primarily as precartilage primordia in the trunk and the limbs, suggesting that these cells were multipotential. Therefore, cells are recruited into the MyoD-dependent myogenic lineage through activation of the -20-kb MyoD enhancer and this occurs independently in somites and limb buds.

Asunto(s)

Proteínas de Unión al ADN , Músculo Esquelético/embriología , Transactivadores , Animales , Movimiento Celular , Elementos de Facilitación Genéticos , Extremidades/embriología , Regulación del Desarrollo de la Expresión Génica , Hibridación in Situ , Operón Lac , Ratones , Ratones Noqueados , Ratones Transgénicos , Proteínas Musculares/genética , Proteínas Musculares/metabolismo , Músculo Esquelético/citología , Músculo Esquelético/metabolismo , Proteína MioD/genética , Proteína MioD/metabolismo , Factor 5 Regulador Miogénico , Somitos/citología , Somitos/metabolismo , Células Madre/citología , Células Madre/metabolismo

RESUMEN

The mdx mouse, a mouse model of Duchenne muscular dystrophy, carries a loss-of-function mutation in dystrophin, a component of the membrane-associated dystrophin-glycoprotein complex. Unlike humans, mdx mice rarely display cardiac abnormalities and exhibit dystrophic changes only in a small number of heavily used skeletal muscle groups. By contrast, mdx:MyoD-/- mice lacking dystrophin and the skeletal muscle-specific bHLH transcription factor MyoD display a severe skeletal myopathy leading to widespread dystrophic changes in skeletal muscle and premature death around 1 year of age. The severely increased phenotype of mdx:MyoD-/- muscle is a consequence of impaired muscle regeneration caused by enhanced satellite cell self-renewal. Here we report that mdx:MyoD-/- mice developed a severe cardiac myopathy with areas of necrosis associated with hypertrophied myocytes. Moreover, heart tissue from mdx:MyoD-/- mice exhibited constitutive activation of stress-activated signaling components, similar to in vitro models of cardiac myocyte adaptation. Taken together, these results support the hypothesis that the progression of skeletal muscle damage is a significant contributing factor leading to development of cardiomyopathy.

Asunto(s)

Cardiomiopatías/patología , Distrofina/genética , Proteínas Quinasas Activadas por Mitógenos , Proteína MioD/genética , Animales , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Cardiomegalia , Cardiomiopatías/complicaciones , Fibrosis , Proteínas Quinasas JNK Activadas por Mitógenos , Ratones , Ratones Endogámicos mdx , Ratones Mutantes , Músculo Esquelético/patología , Distrofia Muscular Animal/complicaciones , Distrofia Muscular Animal/patología , Regeneración , Transducción de Señal , Proteínas Quinasas p38 Activadas por Mitógenos

RESUMEN

The retinoblastoma-related p130 protein is a member of a conserved family, consisting of Rb, p107 and p130, which are believed to play important roles in cell-cycle control and cellular differentiation. We have generated a null mutation in p130 by gene targeting and crossed the null allele into Balb/cJ and C57BL/6J strains of mice. In an enriched Balb/cJ genetic background, p130(-/-) embryos displayed arrested growth and died between embryonic days 11 and 13. Histological analysis revealed varying degrees of disorganization in neural and dermamyotomal structures. Immunohistochemistry with antibody reactive with Islet-1 indicated markedly reduced numbers of neurons in the spinal cord and dorsal root ganglia. Immunohistochemistry with antibody reactive with desmin indicated a similar reduction in the number of differentiated myocytes in the myotome. The myocardium of mutant embryos was abnormally thin and resembled an earlier staged two-chambered heart consisting of the bulbus cordis and the ventricular chamber. TUNEL analysis indicated the presence of extensive apoptosis in various tissues including the neural tube, the brain, the dermomyotome, but not the heart. Immunohistochemistry with antibody reactive with PCNA revealed increased cellular proliferation in the neural tube and the brain, and decreased proliferation in the heart. The placentas of p130(-/-) embryos did not display elevated apoptosis and were indistinguishable from wild type suggesting that the phenotype was not due to placental failure. Following a single cross with the C57BL/6 mice, p130(-/-) animals were derived that were viable and fertile. These results indicate that p130 in a Balb/cJ genetic background plays an essential role that is required for normal development. Moreover, our experiments establish that second-site modifier genes exist that have an epistatic relationship with p130.

Asunto(s)

Desarrollo Embrionario y Fetal/genética , Muerte Fetal/genética , Fosfoproteínas/fisiología , Proteínas , Proteína de Retinoblastoma/fisiología , Animales , Apoptosis , Cruzamientos Genéticos , Embrión de Mamíferos/citología , Embrión de Mamíferos/patología , Embrión de Mamíferos/fisiología , Femenino , Edad Gestacional , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Noqueados , Fenotipo , Fosfoproteínas/deficiencia , Fosfoproteínas/genética , Embarazo , Proteína de Retinoblastoma/deficiencia , Proteína de Retinoblastoma/genética , Proteína p130 Similar a la del Retinoblastoma , Especificidad de la Especie

RESUMEN

To investigate the function of the Rb-related p107 gene, a null mutation in p107 was introduced into the germ line of mice and bred into a BALB/cJ genetic background. Mice lacking p107 were viable and fertile but displayed impaired growth, reaching about 50% of normal weight by 21 days of age. Mutant mice exhibited a diathetic myeloproliferative disorder characterized by ectopic myeloid hyperplasia in the spleen and liver. Embryonic p107(-/-) fibroblasts and primary myoblasts isolated from adult p107(-/-) mice displayed a striking twofold acceleration in doubling time. However, cell sort analysis indicated that the fraction of cells in G1, S, and G2 was unaltered, suggesting that the different phases of the cell cycle in p107(-/-) cells was uniformly reduced by a factor of 2. Western analysis of cyclin expression in synchronized p107(-/-) fibroblasts revealed that expression of cyclins E and A preceded that of D1. Mutant embryos expressed approximately twice the normal level of Rb, whereas p130 levels were unaltered. Lastly, mutant mice reverted to a wild-type phenotype following a single backcross with C57BL/6J mice, suggesting the existence of modifier genes that have potentially epistatic relationships with p107. Therefore, we conclude that p107 is an important player in negatively regulating the rate of progression of the cell cycle, but in a strain-dependent manner.

Asunto(s)

Ciclo Celular/genética , Trastornos del Crecimiento/genética , Trastornos Linfoproliferativos/genética , Proteínas Nucleares/genética , Animales , Células Cultivadas , Cruzamientos Genéticos , Ciclinas/metabolismo , Citometría de Flujo , Histocitoquímica , Inmunohistoquímica , Cinética , Hígado/patología , Ratones , Ratones Noqueados , Fenotipo , ARN Mensajero/genética , Proteína p107 Similar a la del Retinoblastoma , Bazo/patología

RESUMEN

We have isolated and studied the expression pattern of Xemx1 and Xemx2 genes in Xenopus laevis. Xemx genes are the homologues of mouse Emx genes, related to Drosophila empty spiracles. They are expressed in selected regions of the developing brain, particularly in the telencephalon, and, outside the brain, in the otic vesicles, olfactory placodes, visceral arches and the developing excretory system. We also report on experiments concerning the tissue and molecular signals responsible for their activation in competent ectoderm. Xemx genes are activated in ectoderm conjugated with head organizer tissue, but not with tail organizer tissue. Furthermore, they are not activated in animal cap either by noggin or by Xnr3, thus suggesting that a different inducer or the integration of several signals may be responsible for their activation.

Asunto(s)

Tipificación del Cuerpo/genética , Inducción Embrionaria/genética , Proteínas de Homeodominio/genética , Telencéfalo/embriología , Xenopus laevis/embriología , Xenopus laevis/genética , Secuencia de Aminoácidos , Animales , Tipificación del Cuerpo/fisiología , Encéfalo/embriología , Encéfalo/metabolismo , Clonación Molecular , Ectodermo/fisiología , Regulación del Desarrollo de la Expresión Génica , Proteínas de Homeodominio/biosíntesis , Datos de Secuencia Molecular , Telencéfalo/metabolismo , Factores de Transcripción

RESUMEN

Mounting evidence supports the notion that Myf-5 and MyoD play unique roles in the development of epaxial (originating in the dorso-medial half of the somite, e.g. back muscles) and hypaxial (originating in the ventro-lateral half of the somite, e.g. limb and body wall muscles) musculature. To further understand how Myf-5 and MyoD genes cooperate during skeletal muscle specification, we examined and compared the expression pattern of MyoD-lacZ (258/2.5lacZ and MD6.0-lacZ) transgenes in wild-type, Myf-5, and MyoD mutant embryos. We found that the delayed onset of muscle differentiation in the branchial arches, tongue, limbs, and diaphragm of MyoD-/- embryos was a consequence of a reduced ability of myogenic precursor cells to progress through their normal developmental program and not because of a defect in migration of muscle progenitor cells into these regions. We also found that myogenic precursor cells for back, intercostal, and abdominal wall musculature in Myf-54-/- embryos failed to undergo normal translocation or differentiation. By contrast, the myogenic precursors of intercostal and abdominal wall musculature in MyoD-/- embryos underwent normal translocation but failed to undergo timely differentiation. In conclusion, these observations strongly support the hypothesis that Myf-5 plays a unique role in the development of muscles arising after translocation of epithelial dermamyotome cells along the medial edge of the somite to the subjacent myotome (e.g., back or epaxial muscle) and that MyoD plays a unique role in the development of muscles arising from migratory precursor cells (e.g., limb and branchial arch muscles, tongue, and diaphragm). In addition, the expression pattern of MyoD-lacZ transgenes in the intercostal and abdominal wall muscles of Myf-5-/- and MyoD-/- embryos suggests that appropriate development of these muscles is dependent on both genes and, therefore, these muscles have a dual embryonic origin (epaxial and hypaxial).

Asunto(s)

Proteínas de Unión al ADN , Proteínas Musculares/fisiología , Músculos/embriología , Proteína MioD/fisiología , Proteínas del Tejido Nervioso , Transactivadores , Músculos Abdominales/metabolismo , Animales , Diferenciación Celular , Linaje de la Célula , Cruzamientos Genéticos , Desmina/metabolismo , Embrión de Mamíferos/anatomía & histología , Embrión de Mamíferos/metabolismo , Regulación del Desarrollo de la Expresión Génica , Genotipo , Inmunohistoquímica , Hibridación in Situ , Proteínas de Filamentos Intermediarios/metabolismo , Ratones , Ratones Transgénicos , Proteínas Musculares/metabolismo , Músculos/metabolismo , Proteína MioD/metabolismo , Factor 5 Regulador Miogénico , Cadenas Pesadas de Miosina/metabolismo , Nestina , Factores de Tiempo , Distribución Tisular , beta-Galactosidasa/metabolismo

RESUMEN

The myogenic progenitors of epaxial (paraspinal and intercostal) and hypaxial (limb and abdominal wall) musculature are believed to originate in dorsal-medial and ventral-lateral domains, respectively, of the developing somite. To investigate the hypothesis that Myf-5 and MyoD have different roles in the development of epaxial and hypaxial musculature, we further characterized myogenesis in Myf-5- and MyoD-deficient embryos by several approaches. We examined expression of a MyoD-lacZ transgene in Myf-5 and MyoD mutant embryos to characterize the temporal-spatial patterns of myogenesis in mutant embryos. In addition, we performed immunohistochemistry on sectioned Myf-5 and MyoD mutant embryos with antibodies reactive with desmin, nestin, myosin heavy chain, sarcomeric actin, Myf-5, MyoD and myogenin. While MyoD(-/-) embryos displayed normal development of paraspinal and intercostal muscles in the body proper, muscle development in limb buds and brachial arches was delayed by about 2.5 days. By contrast, Myf-5(-/-) embryos displayed normal muscle development in limb buds and brachial arches, and markedly delayed development of paraspinal and intercostal muscles. Although MyoD mutant embryos exhibited delayed development of limb musculature, normal migration of Pax-3-expressing cells into the limb buds and normal subsequent induction of Myf-5 in myogenic precursors was observed. These results suggest that Myf-5 expression in the limb is insufficient for the normal progression of myogenic development. Taken together, these observations strongly support the hypothesis that Myf-5 and MyoD play unique roles in the development of epaxial and hypaxial muscle, respectively.

Asunto(s)

Extremidades/embriología , Proteínas Musculares/fisiología , Músculo Esquelético/embriología , Proteína MioD/fisiología , Columna Vertebral/embriología , Transactivadores , Factores de Transcripción , Animales , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Regulación del Desarrollo de la Expresión Génica , Ratones , Ratones Mutantes , Ratones Transgénicos , Músculo Esquelético/metabolismo , Factor 5 Regulador Miogénico , Factor de Transcripción PAX3 , Factores de Transcripción Paired Box , Células Madre/fisiología , Transgenes , beta-Galactosidasa/genética , beta-Galactosidasa/metabolismo

RESUMEN

To investigate the function of MyoD in adult skeletal muscle, we interbred MyoD mutant mice with mdx mice, a model for Duchenne and Becker muscular dystrophy. Mice lacking both MyoD and dystrophin displayed a marked increase in severity of myopathy leading to premature death, suggesting a role for MyoD in muscle regeneration. Examination of MyoD mutant muscle revealed elevated numbers of myogenic cells; however, myoblasts derived from these cells displayed normal differentiation potential in vitro. Following injury, MyoD mutant muscle was severely deficient in regenerative ability, and we observed a striking reduction in the in vivo proliferation of myogenic cells during regeneration. Therefore, we propose that the failure of MyoD-deficient muscle to regenerate efficiently is not caused by a reduction in numbers of satellite cells, the stem cells of adult skeletal muscle, but results from an increased propensity for stem-cell self-renewal rather than progression through the myogenic program.

Asunto(s)

Músculo Esquelético/fisiología , Proteína MioD/fisiología , Regeneración/fisiología , Animales , División Celular , Células Cultivadas , Distrofina/genética , Distrofina/metabolismo , Eliminación de Gen , Ratones , Ratones Endogámicos mdx , Músculo Esquelético/citología , Músculo Esquelético/ultraestructura , Proteína MioD/genética , Células Madre/fisiología