RESUMEN
An antifungal protein with a molecular mass of 14.3 kDa was isolated from the seeds of butterfly pea (Clitoria ternatea) and designated as Ct protein. The antifungal protein was purified using different methods including ammonium sulphate precipitation, ion exchange chromatography on DEAE-cellulose and gel filtration on Sephadex G-50 column. Ct protein formed a single colourless rod-shaped crystal by hanging drop method after 7 days of sample loading. The protein showed lytic activity against Micrococcus luteus and broad-spectrum, fungicidal activity, particularly against the most clinically relevant yeasts, such as Cryptococcus neoformans, Cryptococcus albidus, Cryptococcus laurentii, Candida albicans and Candida parapsilosis. It also exerted an inhibitory activity on mycelial growth in several mould species including Curvularia sp., Alternaria sp., Cladosporium sp., Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Rhizopus sp., and Sclerotium sp. The present study adds to the literature on novel seed proteins with antifungal activity.
Asunto(s)
Antifúngicos , Clitoria/química , Hongos/crecimiento & desarrollo , Muramidasa , Proteínas de Plantas , Semillas/química , Antifúngicos/química , Antifúngicos/aislamiento & purificación , Antifúngicos/farmacología , Muramidasa/química , Muramidasa/aislamiento & purificación , Muramidasa/farmacología , Proteínas de Plantas/química , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/farmacologíaRESUMEN
UNLABELLED: Ameloblastomas are locally aggressive, benign odontogenic neoplasms having a wide variety of histologic patterns. It is essential to distinguish between the three clinical types of ameloblastomas - the intra-osseous solid lesion, the unicystic type and the extra-osseous lesion, as they differ in their biological behaviour and rate of recurrence and therefore require different forms of treatment. The case presented here is of a 9-year-old boy who reported to the department with pain and swelling along the right side of the mandible of 3 months'duration. Previous histopathological examination of the lesion, performed at a local hospital, produced a picture consistent with unicystic ameloblastoma. The lesion was managed by enucleation and the patient has been followed up for the past 5 years. Radiographic and clinical examinations reveal signs of healing without recurrence. CLINICAL RELEVANCE: Conservative management of unicystic ameloblastomas may be justified in children provided that the patient can be followed up at regular intervals.