RESUMEN
The Kv4 subfamily of voltage-gated potassium channels is responsible for the transient A-type potassium current that operates at subthreshold membrane potentials to control membrane excitability. Arachidonic acid was shown recently to modulate both the peak amplitude and kinetics of the hippocampal A-current. However, in Xenopus oocytes, arachidonic acid only inhibited the peak amplitude of Kv4 current without modifying its kinetics. These results suggest the existence of Kv4 auxiliary subunit(s) in native cells. We report here a K-channel interacting protein (KChIP)-dependent kinetic modulation of Kv4.2 current in Chinese hamster ovary cells and Kv4.2 and Kv4.3 currents in Xenopus oocytes by arachidonic acid at physiological concentrations. This concentration-dependent effect of arachidonic acid resembled that observed in cerebellar granule neurons and was fully reversible. Other fatty acids, including a nonhydrolyzable inhibitor of both lipooxygenase and cyclooxygenase, 5,8,11,14-eicosatetraynoic acid (ETYA), also mimicked arachidonic acid in modulating Kv4.3 and Kv4.3/KChIP1 currents. Compared with another transient potassium current formed by Kv1.1/Kvbeta1, Kv4.3/KChIP1 current was much more sensitive to arachidonic acid. Association between KChIP1 and Kv4.2 or Kv4.3 was not altered in the presence of 10 microm ETYA as measured by immunoprecipitation and association-dependent growth in yeast. Our data suggest that the KChIP proteins represent a molecular entity for the observed difference between arachidonic acid effects on A-current kinetics in heterologous cells and in native cells and are consistent with the notion that KChIP proteins modulate the subthreshold A-current in neurons.
Asunto(s)
Ácido Araquidónico/farmacología , Proteínas de Unión al Calcio/metabolismo , Canales de Potasio con Entrada de Voltaje , Canales de Potasio/metabolismo , Ácido 5,8,11,14-Eicosatetrainoico/farmacología , Animales , Células CHO , Proteínas de Unión al Calcio/genética , Células Cultivadas , Cricetinae , Relación Dosis-Respuesta a Droga , Ácidos Grasos/farmacología , Humanos , Proteínas de Interacción con los Canales Kv , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Neuronas/citología , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Oocitos/metabolismo , Técnicas de Placa-Clamp , Potasio/metabolismo , Canales de Potasio/genética , Unión Proteica/efectos de los fármacos , Subunidades de Proteína , Ratas , Ratas Sprague-Dawley , Canales de Potasio Shal , Transfección , Técnicas del Sistema de Dos Híbridos , Xenopus laevisRESUMEN
The pore-forming alpha subunits of many ion channels are associated with auxiliary subunits that influence channel expression, targeting, and function. Several different auxiliary (beta) subunits for large conductance calcium-dependent potassium channels of the Slowpoke family have been reported, but none of these beta subunits is expressed extensively in the nervous system. We describe here the cloning and functional characterization of a novel Slowpoke beta4 auxiliary subunit in human and mouse, which exhibits only limited sequence homology with other beta subunits. This beta4 subunit coimmunoprecipitates with human and mouse Slowpoke. beta4 is expressed highly in human and monkey brain in a pattern that overlaps strikingly with Slowpoke alpha subunit, but in contrast to other Slowpoke beta subunits, it is expressed little (if at all) outside the nervous system. Also in contrast to other beta subunits, beta4 downregulates Slowpoke channel activity by shifting its activation range to more depolarized voltages and slowing its activation kinetics. beta4 may be important for the critical roles played by Slowpoke channels in the regulation of neuronal excitability and neurotransmitter release.
Asunto(s)
Regulación hacia Abajo/genética , Neuronas/metabolismo , Canales de Potasio Calcio-Activados , Canales de Potasio/genética , Canales de Potasio/metabolismo , Secuencia de Aminoácidos , Animales , Línea Celular , Caribdotoxina/farmacología , Clonación Molecular , Electrofisiología , Epítopos/genética , Expresión Génica/fisiología , Haplorrinos , Humanos , Hibridación in Situ , Activación del Canal Iónico/efectos de los fármacos , Activación del Canal Iónico/fisiología , Riñón/citología , Cinética , Subunidades alfa de los Canales de Potasio de Gran Conductancia Activados por Calcio , Subunidades beta de los Canales de Potasio de Gran Conductancia Activados por el Calcio , Canales de Potasio de Gran Conductancia Activados por el Calcio , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Ratones , Datos de Secuencia Molecular , Neuronas/química , Péptidos/farmacología , Canales de Potasio/química , Estructura Cuaternaria de Proteína , ARN Mensajero/análisis , Análisis de Secuencia de ADNRESUMEN
Voltage-activated K+ channels are integral membrane proteins that open or close a K(+)-selective pore in response to changes in transmembrane voltage. Although the S4 region of these channels has been implicated as the voltage sensor, little is known about how opening and closing of the pore is accomplished. We explored the gating process by introducing cysteines at various positions thought to lie in or near the pore of the Shaker K+ channel, and by testing their ability to be chemically modified. We found a series of positions in the S6 transmembrane region that react rapidly with water-soluble thiol reagents in the open state but not the closed state. An open-channel blocker can protect several of these cysteines, showing that they lie in the ion-conducting pore. At two of these sites, Cd2+ ions bind to the cysteines without affecting the energetics of gating; at a third site, Cd2+ binding holds the channel open. The results suggest that these channels open and close by the movement of an intracellular gate, distinct from the selectivity filter, that regulates access to the pore.
Asunto(s)
Membrana Celular/fisiología , Cisteína/farmacología , Mutación/genética , Canales de Potasio/fisiología , Animales , Cadmio/farmacología , Membrana Celular/efectos de los fármacos , Modelos Moleculares , Canales de Potasio/efectos de los fármacosRESUMEN
The intracellular segment of the Shaker K+ channel between transmembrane domains S4 and S5 has been proposed to form at least part of the receptor for the tethered N-type inactivation "ball." We used the approach of cysteine substitution mutagenesis and chemical modification to test the importance of this region in N-type inactivation. We studied N-type inactivation or the block by a soluble inactivation peptide ("ball peptide") before and after chemical modification by methanethiosulfonate reagents. Particularly at position 391, chemical modification altered specifically the kinetics of ball peptide binding without altering other biophysical properties of the channel. Results with reagents that attach different charged groups at 391 C suggested that there are both electrostatic and steric interactions between this site and the ball peptide. These findings identify this site to be in or near the receptor site for the inactivation ball. At many of the other positions studied, modification noticeably inhibited channel current. The accessible cysteines varied in the state-dependence of their modification, with five- to tenfold changes in reactions rate depending on the gating state of the channel.
Asunto(s)
Mutación/fisiología , Canales de Potasio/metabolismo , Secuencia de Aminoácidos , Animales , Línea Celular , Cisteína/genética , Cisteína/metabolismo , Electrofisiología , Humanos , Indicadores y Reactivos , Activación del Canal Iónico , Ratones , Datos de Secuencia Molecular , Mutagénesis , Canales de Potasio/genéticaRESUMEN
With prolonged stimulation, voltage-activated K+ channels close by a gating process called inactivation. This inactivation gating can occur by two distinct molecular mechanisms: N-type, in which a tethered particle blocks the intracellular mouth of the pore, and C-type, which involves a closure of the external mouth. The functional motion involved in C-type inactivation was studied by introducing cysteine residues at the outer mouth of Shaker K+ channels through mutagenesis, and by measuring state-dependent changes in accessibility to chemical modification. Modification of three adjacent residues in the outer mouth was 130-10,000-fold faster in the C-type inactivated state than in the closed state. At one position, state-dependent bridging or crosslinking between subunits was also possible. These results give a consistent picture in which C-type inactivation promotes a local rearrangement and constriction of the channel at the outer mouth.
Asunto(s)
Activación del Canal Iónico/fisiología , Canales de Potasio/química , Canales de Potasio/fisiología , Línea Celular , Reactivos de Enlaces Cruzados , Cisteína/química , Ditiotreitol/farmacología , Conductividad Eléctrica , Embrión de Mamíferos , Humanos , Riñón , Sustancias Macromoleculares , Potenciales de la Membrana , Metilmetanosulfonato/farmacología , Mutagénesis Sitio-Dirigida , Canales de Potasio/genética , Conformación Proteica , Relación Estructura-Actividad , TransfecciónRESUMEN
Electrophysiological study of transiently transfected cells requires the identification of individual cells that express the protein of interest. We describe a simple, quick and inexpensive method for visually identifying cells that have been co-transfected with an expression plasmid for a lymphocyte surface antigen (CD8-alpha). Transfected cells are incubated briefly with polystyrene microspheres (4.5 microns diameter) that have been precoated with antibody to CD8. Cells expressing CD8 on their surface are decorated with many beads and are thus readily distinguishable from untransfected cells. Beads already coated with antibody are available commercially. The method takes less than five minutes and requires no reagent preparation or special equipment for visualization of the beads.
Asunto(s)
Anticuerpos , Microesferas , Canales de Potasio/fisiología , Transfección , Antígenos CD8/genética , Antígenos CD8/inmunología , Línea Celular , Electrofisiología , Electroporación , Embrión de Mamíferos , Humanos , Riñón , Plásmidos , Poliestirenos , Canales de Potasio/genética , beta-Galactosidasa/análisis , beta-Galactosidasa/genéticaRESUMEN
Substitution of a cysteine in the extracellular mouth of the pore of the Shaker-delta K+ channel permits allosteric inhibition of the channel by Zn2+ or Cd2+ ions at micromolar concentrations. Cd2+ binds weakly to the open state but drives the channel into the slow (C-type) inactivated state, which has a Kd for Cd2+ of approximately 0.2 microM. There is a 45,000-fold increase in affinity when the channel changes from open to inactivated. These results indicate that C-type inactivation involves a structural change in the external mouth of the pore. This structural change is reflected in the T449C mutant as state-dependent metal affinity, which may result either from a change in proximity of the introduced cysteine residues of the four subunits or from a change of the exposure of this residue on the surface of the protein.
Asunto(s)
Canales de Potasio/metabolismo , Regulación Alostérica/genética , Animales , Sitios de Unión , Fenómenos Biofísicos , Biofisica , Cadmio/metabolismo , Cadmio/farmacología , Línea Celular , Cisteína/genética , Cisteína/metabolismo , Humanos , Cinética , Modelos Biológicos , Mutagénesis Sitio-Dirigida , Bloqueadores de los Canales de Potasio , Canales de Potasio/genética , Unión Proteica , Conformación Proteica , Ingeniería de Proteínas , Zinc/metabolismoRESUMEN
We investigated whether the cysteine residues in Shaker potassium (K+) channels are essential for activation and inactivation gating or for modulation of activation gating by external zinc (Zn2+). Mutants of the Shaker K+ channel were prepared in which all seven cysteine residues were replaced (C-less). These changes were made in both wild-type Shaker H4 channels and in a deletion mutant (delta 6-46) lacking N-type ("fast") inactivation. Replacement of all cysteines left most functional properties of the K+ currents unaltered. The most noticeable difference between the C-less and wild-type currents was the faster C-type inactivation of the C-less channel which could be attributed largely to the mutation of Cys462. This is consistent with the effects of previously reported mutations of nearby residues in the S6 region. There were also small changes in the activation gating of C-less currents. Modulation by external Zn2+ of the voltage dependence and rate of activation gating is preserved in the C-less channels, indicating that none of the cysteines in the Shaker K+ channel plays an important role in Zn2+ modulation.
Asunto(s)
Péptidos/metabolismo , Canales de Potasio/metabolismo , Animales , Fenómenos Biofísicos , Biofisica , Línea Celular , Cisteína/química , Cisteína/genética , Humanos , Activación del Canal Iónico/efectos de los fármacos , Cinética , Mutagénesis Sitio-Dirigida , Oocitos/metabolismo , Péptidos/antagonistas & inhibidores , Péptidos/genética , Bloqueadores de los Canales de Potasio , Canales de Potasio/genética , Eliminación de Secuencia , Canales de Potasio de la Superfamilia Shaker , Xenopus laevis , Zinc/farmacologíaRESUMEN
The nicotinic acetylcholine receptor (AChR) is a pentameric transmembrane protein (alpha 2 beta gamma delta) that binds the neurotransmitter acetylcholine (ACh) and transduces this binding into the opening of a cation selective channel. The agonist, competitive antagonist, and snake toxin binding functions of the AChR are associated with the alpha subunit (Kao et al., 1984; Tzartos and Changeux, 1984; Wilson et al., 1985; Kao and Karlin, 1986; Pederson et al., 1986). We used site-directed mutagenesis and expression of AChR in Xenopus oocytes to identify amino acid residues critical for ligand binding and channel activation. Several mutations in the alpha subunit sequence were constructed based on information from sequence homology and from previous biochemical (Barkas et al., 1987; Dennis et al., 1988; Middleton and Cohen, 1990) and spectroscopic (Pearce and Hawrot, 1990; Pearce et al., 1990) studies. We have identified one mutation, Tyr190 to Phe (Y190F), that had a dramatic effect on ligand binding and channel activation. These mutant channels required more than 50-fold higher concentrations of ACh for channel activation than did wild type channels. This functional change is largely accounted for by a comparable shift in the agonist binding affinity, as assessed by the ability of ACh to compete with alpha-bungarotoxin binding. Other mutations at nearby conserved positions of the alpha subunit (H186F, P194S, Y198F) produce less dramatic changes in channel properties. Our results demonstrate that ligand binding and channel gating are separable properties of the receptor protein, and that Tyr190 appears to play a specific role in the receptor site for acetylcholine.
Asunto(s)
Canales Iónicos/fisiología , Mutagénesis Sitio-Dirigida , Oocitos/fisiología , Receptores Nicotínicos/fisiología , Secuencia de Aminoácidos , Animales , Unión Competitiva , Bungarotoxinas/metabolismo , Membrana Celular/metabolismo , Femenino , Humanos , Canales Iónicos/efectos de los fármacos , Canales Iónicos/genética , Sustancias Macromoleculares , Ratones , Datos de Secuencia Molecular , Oocitos/efectos de los fármacos , Plásmidos , ARN/genética , Receptores Nicotínicos/genética , Receptores Nicotínicos/metabolismo , Homología de Secuencia de Ácido Nucleico , Transcripción Genética , Xenopus laevisRESUMEN
The active site of voltage-activated potassium channels is a transmembrane aqueous pore that permits ions to permeate the cell membrane in a rapid yet highly selective manner. A useful probe for the pore of potassium-selective channels is the organic ion tetraethylammonium (TEA), which binds with millimolar affinity to the intracellular opening of the pore and blocks potassium current. In the potassium channel encoded by the Drosophila Shaker gene, an amino acid residue that specifically affects the affinity for intracellular TEA has now been identified by site-directed mutagenesis. This residue is in the middle of a conserved stretch of 18 amino acids that separates two locations that are both near the external opening of the pore. These findings suggest that this conserved region is intimately involved in the formation of the ion conduction pore of voltage-activated potassium channels. Further, a stretch of only eight amino acid residues must traverse 80 percent of the transmembrane electric potential difference.
Asunto(s)
Mutagénesis Sitio-Dirigida , Canales de Potasio/fisiología , Compuestos de Tetraetilamonio/farmacología , Secuencia de Aminoácidos , Animales , Sitios de Unión , Drosophila/genética , Genes , Potenciales de la Membrana , Modelos Estructurales , Datos de Secuencia Molecular , Canales de Potasio/efectos de los fármacos , Canales de Potasio/genética , Conformación Proteica , TetraetilamonioRESUMEN
Nitrendipine, nifedipine and verapamil inhibit the in vitro formation of irreversibly sickled cells. Using a method of forming both dehydrated cells and irreversibly sickled cells in vitro by repeated cycles of sickling and unsickling, the effects of several drugs in inhibiting the formation of these cells were studied. Drugs known as Ca2+ channel antagonists, such as nitrendipine, nifedipine and verapamil were found to inhibit these reactions. Other types of calcium channel blockers, such as lanthanum and zinc, did not inhibit the formation of these cells. The potency of drugs to inhibit irreversibly sickled cell formation was related to the potency of inhibition of calmodulin-activated phosphodiesterase.
Asunto(s)
Anemia de Células Falciformes/sangre , Bloqueadores de los Canales de Calcio/farmacología , Eritrocitos Anormales/efectos de los fármacos , Adulto , Calcio/sangre , Calmodulina/fisiología , Deshidratación/sangre , Eritrocitos Anormales/metabolismo , Humanos , Técnicas In Vitro , Lantano/farmacología , Nifedipino/análogos & derivados , Nifedipino/farmacología , Nitrendipino , Inhibidores de Fosfodiesterasa , Potasio/sangre , Verapamilo/farmacología , Zinc/farmacologíaRESUMEN
We describe a simple procedure for the microassay of testosterone 5 alpha-reductase in homogenates of rat brain. This enzyme converts testosterone to dihydrotestosterone. We have used this assay to characterize the enzymatic activity and to map its distribution. The apparent Km is 4.1 x 10(-6) M and the Vmax is 85.6 pmol/mg protein/h. The pH optimum is broad and extends from pH 6.0 to 8.0. For the brain regions surveyed, testosterone 5 alpha-reductase activity varied over a 10-fold range. The highest activities were observed in homogenates of the midbrain and pons (37-39 pmol/mg protein/h). The lowest were seen in homogenates of the thalamus, caudate nucleus, frontal cortex, hippocampus, hypothalamus, olfactory tubercle, and preoptic area (3-7 pmol/mg protein/h).
Asunto(s)
3-Oxo-5-alfa-Esteroide 4-Deshidrogenasa/metabolismo , Encéfalo/enzimología , Oxidorreductasas/metabolismo , Animales , Ácido Edético/farmacología , Congelación , Concentración de Iones de Hidrógeno , Cinética , Masculino , Ratas , Ratas EndogámicasRESUMEN
Testosterone 5 alpha-reductase, the enzyme that converts testosterone to 5 alpha-dihydrotestosterone, is present in the spinal cord of Xenopus laevis. In adult males the enzymatic activity is optimal at pH 7.4 and 27 degrees C; the apparent Km is 2.0 x 10(-5) M and the Vmax is 10.0 pmol/mg protein/h. Enzymatic activity was assayed in segments of the spinal cord in each of four groups: control untreated males, females, castrated males, and sexually active clasping males. Striking differences in both the amount of dihydrotestosterone produced with time and in the pattern of its distribution were seen in spinal cords of clasping males compared with those of the other groups. The differences are greatest in the basal medulla and rostral segments of the spinal cord. Neurons in these segments innervate the muscles primarily involved in clasping.
Asunto(s)
3-Oxo-5-alfa-Esteroide 4-Deshidrogenasa/metabolismo , Oxidorreductasas/metabolismo , Médula Espinal/enzimología , 3-Oxo-5-alfa-Esteroide 4-Deshidrogenasa/aislamiento & purificación , Animales , Castración , Cristalización , Femenino , Concentración de Iones de Hidrógeno , Cinética , Masculino , Conducta Sexual Animal , XenopusRESUMEN
The neural control of the clasp reflex in male Xenopus laevis has been studied by using anatomical, electrophysiological, and biochemical techniques. Neurons in spinal segment 2 of castrated males accumulate label after injection of [3H]-dihydrotestosterone; these neurons are distributed within the rostral portions of the motoneuronal pools of the sternoradialis and flexor carpi radialis muscles. In vitro recordings from the nerve to the sternoradialis muscle in the isolated spinal cord preparation from castrated male Xenopus showed increased activation to paired dorsal root stimulation after addition of dihydrotestosterone to the bath. This increase could be prevented by prior administration of cycloheximide. The reducing enzyme testosterone 5 alpha-reductase is present and is selectively distributed in male Xenopus spinal cord. It is speculated that the androgens may alter patterns of neuronal activity leading to the "clasp" muscles and thereby influence myosin types within these muscles.