RESUMEN

To study chemical constituents contained in roots of Caesalpinia millettii by HPLC. Six homoisoflavonoids were identified by spectroscopic data and physicochemical property as eucomin (1), intricatinol (2), 8-methoxybonducellin (3), bonducellin (4), 8-methoxyisobonducellin (5) and 3-(4-methoxybenzyl) -5, 7-dimethoxychroman-4-one (6). All compounds were separated from the root of this genus for the first time. An antibacterial screening was made on eight monomeric compounds. Among them, 8-methoxyisobonducellin, intricatinol, bergenin, hyperoside and 11-O-galloylbergenin showed a inhibitory effect on Staphylococcus aureus, Klebsiella Peneumoniae, Beta streptococcus and Aeruginosus bacillus.

Asunto(s)

Antibacterianos , Caesalpinia , Química , Cromatografía Líquida de Alta Presión , Isoflavonas , Farmacología , Pruebas de Sensibilidad Microbiana , Raíces de Plantas , Química

RESUMEN

The column chromatography on silica gel, sephadex LH-20 preparative HPLC were used to separate and purify the compounds from the stems of Dendrobium candidum. Twenty compounds were isolated and identified as 3,4'-dihydroxy-5-methoxybibenzyl(1), dihydroresveratrol(2), dendromoniliside E(3), denbinobin(4),2,4,7-trihydroxy-9, 10-dihydrophenanthrene(5), aduncin(6), (-)-loliolide(7), adenosine(8), uridine(9), guanosine(10), sucrose(11), 5-hydroxymethyl-furaldehyde(12), n-octacostyl ferulate(13), defuscin(14), n-triacontyl cis-p-coumarate(15), daucosterol(16), beta-sitosterol(17), hexadecanoic acid(18), hentriacontane(19), and heptadecanoic acid(20). Their structures were elucidated on the basis of spectroscopic data and physicochemical properties. All of the compounds were isolated from this plant for the first time.

Asunto(s)

Dendrobium , Química , Estructura Molecular , Extractos Vegetales , Química

RESUMEN

<p><b>OBJECTIVE</b>To study the chemical constituents in the rhizoma of Iris dichotoma.</p><p><b>METHOD</b>The chemical constituents were isolated by various column chromatographic methods. The structures of the compounds were elucidated on the basis of physiochemical properties and spectral analysis.</p><p><b>RESULT</b>Eleven compounds, hispidulin (1), rhamnocitrin (2), iristectorigenin A (3), 4', 5, 7, 8-tetrahydroxy-6-methoxy isoflavone (4), 6-hydroxybiochanin A (5), iristectorin B (6), iristectorigenin A (7), kaempferol-7-methyl ether (8), tamarixetin-7-glucoside (9), iristectorin A (10), 3', 3, 5-trihydroxy-4', 7-dimethoxy-flavone-3-O-beta-D-galactopyranoside (11) were isolated and identified.</p><p><b>CONCLUSION</b>Compounds 1-11 were obtained from this plant for the first time.</p>

Asunto(s)

Iris , Química , Extractos Vegetales

RESUMEN

Objective To study the protective effect of Octreotide against ischemia-reperfusion injury when liver was resected Methods Using the routine Pringle's maneuver hepaticvaseular occlusion,thirty patients for resection of liver with cirrhosis were random divided into two groups:octreotide group(n=15)and physiologic saline group(n=15).In octreotide group,the patients with hepatecto15),the patients were injected 1ml saline in the same way.The variations of postoperative liver function were measured.Plasma MDA and TNF-α after reperfusion for 1h were measured and the hepatic histopathologic alterations were also examined.Results The levels of ALT and AST from the octreotide group increased less than those of the physiologic saline group(P＜0.01).Meanwhile,Plasma MDA and TNF-α from the octreotide group was slightly increased(P＜0.01)and the hepatic histopathologic alterations were significantly decreased.Conclusions Given octreotide by subcutaneous injection before blocking portabepatis show all important protective effect against the hepatic isehemia-repedusion injury for resection of liver.

RESUMEN

To search for bioactive compounds from the flower disc of Helianthus annuus L.,chromatography was used to isolate and purify the chemical constituents, their structures were identified by spectral analysis, MTT method was applied to investigate their cytotoxic activities, some compounds showed moderate cytotoxic activities on SF-268, MCF-7 and HepG2 cell lines. Eleven compounds were obtained from the flower disc of H. annuus, and identified as ent-kaurane-2o, 16α-diol ( 1 ) and entkaurane-15α, 16α-epoxy-17-al-19-oic acid (2), and nine known diterpenes, ent-kaurane-16β-ol (3),phyllocladan-16β-ol (4) , ent-atisan-16α-ol ( 5 ) , grandifloric acid ( 6 ) , angeloylgrandifloric acid ( 7 ) ,ent-kaurane-16-en-19-oic acid (8), ent-kaurane-17-hydroxy-15-en-19-oic acid (9), ent-kaurane-16β,17-dihydroxy-19-oic acid (10), and ciliaric acid (11). Compounds 1 and 2 are new compounds, some compounds showed cytotoxic activities on SF-268, MCF-7 and HepG2 cell lines.

RESUMEN

Aim To study the chemical constituents of Cypripedium tibeticum. Methods Compounds were isolated by repeated silica gel chromatography and purified on Sephadex LH-20 and structures were determined by spectral analysis. Results Cypritibetquinones A and B were isolated from the ethyl acetate residue and their structures were determined as 7-hydroxy-2-methoxy-1, 4-phenanthraquinone ( 1 ) and 7-hydroxy-2,10-dimethoxy-1,4-phenanthraquinone ( 2 ), respectively, by extensive spectral analyses. Conclusion Cypritibetquinones A and B are two new phenanthraquinones.

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Vascular endothelial growth factor (VEGF), one of the most important angiogenic factors, plays an essential role in both physiological and pathological angiogenesis. The VEGF receptor KDR/Flk-1 (a kinase domain receptor) mediates various biological activities of VEGF related to proliferation, differentiation, and migration of endothelial cells. Here we present a novel peptide designated K237-(HTMYYHHYQHHL), which was isolated from a phage-displayed peptide library, binding to KDR with high affinity and specificity. By interfering with the VEGF-KDR interaction, the peptide K237 inhibited proliferation of cultured primary human umbilical vein endothelial cells induced by recombinant human VEGF(165) in a dose-dependent and cell type-specific manner. The peptide also exerted an anti-angiogenesis activity in vivo as revealed using the chick embryo chorioallantoic membrane angiogenesis assay. Moreover, the peptide K237 significantly inhibited the growth of solid tumors implanted beneath the breasts and their metastases to lungs in severe combined immunodeficient mice. Taken together, these findings suggest that the peptide K237 can functionally disrupt the interaction between VEGF and the KDR receptor and cause potent biological effects that include the inhibition of angiogenesis and tumor growth. As a consequence, this peptide (and its future derivatives) may have use as a potential cancer therapy.

Asunto(s)

Endotelio Vascular/citología , Metástasis de la Neoplasia/prevención & control , Neovascularización Patológica/prevención & control , Neovascularización Fisiológica/efectos de los fármacos , Oligopéptidos/farmacología , Fragmentos de Péptidos/farmacología , Biblioteca de Péptidos , Proteínas Tirosina Quinasas/metabolismo , Alantoides/efectos de los fármacos , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Sitios de Unión , Neoplasias de la Mama , División Celular , Células Cultivadas , Embrión de Pollo , Corion/efectos de los fármacos , Cartilla de ADN , ADN Complementario , Factores de Crecimiento Endotelial/farmacología , Endotelio Vascular/efectos de los fármacos , Femenino , Humanos , Péptidos y Proteínas de Señalización Intercelular/farmacología , Linfocinas/farmacología , Oligopéptidos/química , Fragmentos de Péptidos/química , Proteínas Recombinantes/farmacología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Especificidad por Sustrato , Células Tumorales Cultivadas , Venas Umbilicales , Factor A de Crecimiento Endotelial Vascular , Receptor 2 de Factores de Crecimiento Endotelial Vascular/antagonistas & inhibidores , Receptor 2 de Factores de Crecimiento Endotelial Vascular/genética , Factores de Crecimiento Endotelial Vascular

RESUMEN

Objective To explore the new operation approach and the effect of surgical therapy on cardial carcinoma. Methods A total of 78 patients with cardial carcinoma received surgical therapy by the transabdominal route from January 1992 to January 2002, of which 30 cases were treated by total gastrectomy by substitution of P shaped jejunum and 48 cases were treated by subtotal gastrectomy. All patients were followed-up postoperatively from 6 months to 5 years. Results The average length of resected lower part of the esophagus above the tumors was over 5cm, without cancer tissue retained in resected edge. The rate of intramediastinal lymph node metastasis was 18.4%. 5-year survival rate of the patients with total gastrectomy was higher than that of the patients with subtotal gastrectomy, and the morbidity rate of reflux esophagitis of the former was lower than that of the latter. Conclusions The patients of cardial carcinoma in Ⅲ stage or Ⅱstage with lymph node metastasis of 5 or 6 groups should be treated by radical total gastrectomy. The P shaped reconstruction of stomach with jejunum as a replacement satisfies the physiological needs and causes less compications.

RESUMEN

Objective To investigate the chemical constituents in the vines of Piper hancei for obtaining a more comprehensive understanding on its effective components. Methods Compounds were separated by column chromatography with silica gel and polyamide, and their structures were elucidated by spectral analysis and chemical evidence (IR, UV, MS, (~1H-NMR), (~(13)C-NMR)). Results Nine compounds were isolated from the chloroform extract fraction. Their structures were identified as: futoamide (Ⅰ), trichostachine (Ⅱ), retrofractamide A (Ⅲ), pipercide (Ⅳ), guineensine (Ⅴ), piperine (Ⅵ), piperettine (Ⅶ), piperovatine (Ⅷ), and (2E, 4E)-N-isobutyl-7-(3, 4-methylenedioxyphenyl)-hepta-2, 4-dienamide (Ⅸ). (Conclusion )Compounds Ⅱ-Ⅳ and Ⅶ-Ⅸ are isolated from the plant for the first time.

RESUMEN

Objective To study the chemical constitutents of Fomes officinalis and their inhibiting (effect) on thrombin. Methods Compounds were separated by column chromatography with silica gel and polyamide, whose structures were elucidated by spectral analysis and chemical evidence. Results Seven compounds were isolated from the chloroform extract. Their structures were identified as: 3-keto-dehydrosulfurenic (Ⅰ), dehydroeburicoic acid (Ⅱ), eburicoic acid (Ⅲ), sulphurenic acid (Ⅳ), dehydrosulphurenic acid (Ⅴ), dehydroeburiconic acid (Ⅵ), versisponic acid D (Ⅶ). The inhibitory rate of compound Ⅶ on thrombin was 45.36% but others were not obvious. Conclusion Compounds Ⅴ, Ⅶ are isolated from the fungus for the first time. Compound Ⅶ is effective to anti-thrombin at higher concentration, while the remainders are not obvious.

RESUMEN

Objective To investigate the chemical constituents of Polygala tenuifolia. Methods Silica gel column and Sephadex LH-20 column chromatography were used to separate and purify the chemical constituents. The structures were elucidated by spectral data. Results Six compounds were isolated and identified as: 2-hydroxy-4, 6-dimethoxybenzophone (hydrocotoin, Ⅰ), 1, 7-dihydroxy-3-methoxyxanthone (Ⅱ), 1, 7-dihydroxy-2, 3-dimethoxyxanthone (Ⅲ), ?-spinasterol (Ⅳ), 1, 5-anhydro-D-sorbitol (polygitol Ⅴ) and benzoic acid (Ⅵ). Conclusion Compound Ⅳ is isolated from the plant and compound Ⅰ is isolated from the plants of Polygala L. both for the first time.