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1.
Einstein (Sao Paulo) ; 21: eRC0282, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37255062

RESUMEN

Polycystic liver disease, a hereditary pathology, usually manifests as autosomal dominant polycystic kidney disease. The many cysts in the liver cause massive hepatomegaly, majorly affecting the patient's quality of life. In cases of refractory symptoms, liver transplantation is the only treatment choice. A 43-year-old woman was followed up as a hepatology outpatient in August 2020, with a progressive increase in abdominal volume, lower limb edema, and cachexia. The patient was diagnosed with polycystic renal and liver disease with massive hepatomegaly in March 2021, a combined kidney-liver transplant. Liver size represented 13% of the patient's corporal composition, weighing 8.6kg. The patient was discharged on the 7th postoperative day with no complications. Only 10-20% of patients with polycystic liver disease have clinical manifestations, most of which result from hepatomegaly. An increase in liver volume deteriorates liver function until the condition becomes end-stage liver disease, as kidney function is already compromised; liver-kidney transplantation remains the only treatment choice. The case described drew significant attention to the massive hepatomegaly presented in the patient, with the liver representing over 10% of the patient's body weight, approximately five to six times larger than a normal-sized liver.


Asunto(s)
Trasplante de Riñón , Trasplante de Hígado , Femenino , Humanos , Adulto , Hepatomegalia/diagnóstico por imagen , Hepatomegalia/etiología , Trasplante de Riñón/efectos adversos , Calidad de Vida , Riñón
2.
Einstein (São Paulo, Online) ; 21: eRC0282, 2023. graf
Artículo en Inglés | LILACS-Express | LILACS | ID: biblio-1440071

RESUMEN

ABSTRACT Polycystic liver disease, a hereditary pathology, usually manifests as autosomal dominant polycystic kidney disease. The many cysts in the liver cause massive hepatomegaly, majorly affecting the patient's quality of life. In cases of refractory symptoms, liver transplantation is the only treatment choice. A 43-year-old woman was followed up as a hepatology outpatient in August 2020, with a progressive increase in abdominal volume, lower limb edema, and cachexia. The patient was diagnosed with polycystic renal and liver disease with massive hepatomegaly in March 2021, a combined kidney-liver transplant. Liver size represented 13% of the patient's corporal composition, weighing 8.6kg. The patient was discharged on the 7th postoperative day with no complications. Only 10-20% of patients with polycystic liver disease have clinical manifestations, most of which result from hepatomegaly. An increase in liver volume deteriorates liver function until the condition becomes end-stage liver disease, as kidney function is already compromised; liver-kidney transplantation remains the only treatment choice. The case described drew significant attention to the massive hepatomegaly presented in the patient, with the liver representing over 10% of the patient's body weight, approximately five to six times larger than a normal-sized liver.

3.
Obes Res Clin Pract ; 14(4): 326-332, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32690320

RESUMEN

AIM: This study evaluated the genesPNPLA3 and LGALS3 in patients who have undergone bariatric surgery. METHODS: Individuals with NAFLD and NASH were evaluated, the DNA was extracted from total blood for genotyping of rs4644, rs4652 from LGALS3 and rs738409 from PNPLA3 genes, the total RNA was obtained from liver biopsy. For the detection of the molecular targets, real-time PCR through Taqman probes was used. RESULTS: From a total of 46 collected patients, of those 21 (456%) were included as NASH and 25 (544%) as steatosis group. This groups showed significant difference to aspartate aminotransferase (AST), alanine aminotransferase (ALT) and Glutamyl transpeptidase (GGT) (p = 0.0108, p = 0.0090 and p = 0.0044). Regarding to gene expression in studied groups, hepatic steatosis vs NASH, we observed a higher expression of the LGALS3 gene in NASH (p = 0.0273). In addition, patients with C allele in homozygous for rs4644 and rs4652 of LGALS3 gene had higher expression, in NASH group (p = 0.0500 and p = 0.0242, respectively), furthermore for rs4644 both alleles in homozygous showed higher expression (AA/CC vs AC) (p = 0.0500), when analyzed PNPLA3 rs738409, NASH patients with G allele in homozygous had higher expression (p = 0.0494). CONCLUSIONS: Therefore, an increased expression of the LGALS3 gene in patients with NASH may be important in the etiopathogenesis of the disease, as well as the presence of rs4652 and rs4644 SNPs in the regulation of transcriptional levels of the gene in patients with NAFLD and NASH.


Asunto(s)
Cirugía Bariátrica , Proteínas Sanguíneas , Galectinas , Lipasa , Proteínas de la Membrana , Enfermedad del Hígado Graso no Alcohólico , Proteínas Sanguíneas/genética , Galectina 3 , Galectinas/genética , Humanos , Lipasa/genética , Hígado , Proteínas de la Membrana/genética , Enfermedad del Hígado Graso no Alcohólico/genética , Enfermedad del Hígado Graso no Alcohólico/cirugía , Polimorfismo de Nucleótido Simple
4.
Exp Clin Transplant ; 18(5): 641-644, 2020 10.
Artículo en Inglés | MEDLINE | ID: mdl-31250739

RESUMEN

Hepatic artery dissection is an infrequent vascular complication that can arise after orthotopic liver transplant. Most patients with this complication are diagnosed during the intraoperative period or the first days after liver transplant, with an association shown with living-donor liver transplant. In this study, we discuss a rare case of an extrahepatic artery dissection that was successfully managed through surgical excision and arterial revascularization that was diagnosed 4 years after orthotopic liver transplant. Furthermore, we hypothesize on the potential causes of its occurrence.


Asunto(s)
Arteria Hepática/lesiones , Isquemia/etiología , Trasplante de Hígado/efectos adversos , Lesiones del Sistema Vascular/etiología , Adulto , Anastomosis Quirúrgica , Arteria Hepática/diagnóstico por imagen , Arteria Hepática/fisiopatología , Arteria Hepática/cirugía , Humanos , Isquemia/diagnóstico por imagen , Isquemia/fisiopatología , Isquemia/cirugía , Circulación Hepática , Masculino , Persona de Mediana Edad , Reoperación , Factores de Tiempo , Resultado del Tratamiento , Procedimientos Quirúrgicos Vasculares , Lesiones del Sistema Vascular/diagnóstico por imagen , Lesiones del Sistema Vascular/fisiopatología , Lesiones del Sistema Vascular/cirugía
5.
Braz. j. infect. dis ; Braz. j. infect. dis;22(4): 352-354, July-Aug. 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1039217

RESUMEN

ABSTRACT Schistosomiasis affects approximately 207 million people in 76 countries. The association between hepatocellular carcinoma and Schistosoma mansoni infection has been investigated. Studies using animal models suggest that the parasite may accelerate the oncogenic process when combined with other factors, such as hepatitis C virus infection or exposure to a carcinogen. Herein, we report a case series of six hepatocellular carcinoma patients from Northeast Brazil, with negative serology for both hepatitis B and C virus, submitted to liver transplantation, whose explant showed evidence of schistosomal liver fibrosis. Since all patients enrolled in this study were submitted to liver transplantation, we were able to access the whole explanted liver and perform histopathological analysis, which is often not possible in other situations. Although 50% of them showed signs of liver failure, no cirrhosis or any liver disease other than schistosomal fibrosis had been detected. These uncommon findings suggest that Schistosoma mansoni infection might predispose to hepatocellular carcinoma development, regardless of the absence of other risk factors.


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Anciano , Esquistosomiasis mansoni/cirugía , Trasplante de Hígado , Carcinoma Hepatocelular/cirugía , Neoplasias Hepáticas/cirugía , Esquistosomiasis mansoni/complicaciones , Esquistosomiasis mansoni/epidemiología , Brasil/epidemiología , Factores de Riesgo , Distribución por Sexo , Carcinoma Hepatocelular/parasitología , Carcinoma Hepatocelular/patología , Hígado/parasitología , Cirrosis Hepática/parasitología , Cirrosis Hepática/patología , Neoplasias Hepáticas/parasitología , Neoplasias Hepáticas/patología
6.
Braz J Infect Dis ; 22(4): 352-354, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30017854

RESUMEN

Schistosomiasis affects approximately 207 million people in 76 countries. The association between hepatocellular carcinoma and Schistosoma mansoni infection has been investigated. Studies using animal models suggest that the parasite may accelerate the oncogenic process when combined with other factors, such as hepatitis C virus infection or exposure to a carcinogen. Herein, we report a case series of six hepatocellular carcinoma patients from Northeast Brazil, with negative serology for both hepatitis B and C virus, submitted to liver transplantation, whose explant showed evidence of schistosomal liver fibrosis. Since all patients enrolled in this study were submitted to liver transplantation, we were able to access the whole explanted liver and perform histopathological analysis, which is often not possible in other situations. Although 50% of them showed signs of liver failure, no cirrhosis or any liver disease other than schistosomal fibrosis had been detected. These uncommon findings suggest that Schistosoma mansoni infection might predispose to hepatocellular carcinoma development, regardless of the absence of other risk factors.


Asunto(s)
Carcinoma Hepatocelular/cirugía , Neoplasias Hepáticas/cirugía , Trasplante de Hígado , Esquistosomiasis mansoni/cirugía , Adulto , Anciano , Brasil/epidemiología , Carcinoma Hepatocelular/parasitología , Carcinoma Hepatocelular/patología , Femenino , Humanos , Hígado/parasitología , Cirrosis Hepática/parasitología , Cirrosis Hepática/patología , Neoplasias Hepáticas/parasitología , Neoplasias Hepáticas/patología , Masculino , Persona de Mediana Edad , Factores de Riesgo , Esquistosomiasis mansoni/complicaciones , Esquistosomiasis mansoni/epidemiología , Distribución por Sexo
7.
World J Hepatol ; 10(1): 105-115, 2018 Jan 27.
Artículo en Inglés | MEDLINE | ID: mdl-29399284

RESUMEN

AIM: To develop metabonomic models (MMs), using 1H nuclear magnetic resonance (NMR) spectra of serum, to predict significant liver fibrosis (SF: Metavir ≥ F2), advanced liver fibrosis (AF: METAVIR ≥ F3) and cirrhosis (C: METAVIR = F4 or clinical cirrhosis) in chronic hepatitis C (CHC) patients. Additionally, to compare the accuracy of the MMs with the aspartate aminotransferase to platelet ratio index (APRI) and fibrosis index based on four factors (FIB-4). METHODS: Sixty-nine patients who had undergone biopsy in the previous 12 mo or had clinical cirrhosis were included. The presence of any other liver disease was a criterion for exclusion. The MMs, constructed using partial least squares discriminant analysis and linear discriminant analysis formalisms, were tested by cross-validation, considering SF, AF and C. RESULTS: Results showed that forty-two patients (61%) presented SF, 28 (40%) AF and 18 (26%) C. The MMs showed sensitivity and specificity of 97.6% and 92.6% to predict SF; 96.4% and 95.1% to predict AF; and 100% and 98.0% to predict C. Besides that, the MMs correctly classified all 27 (39.7%) and 25 (38.8%) patients with intermediate values of APRI and FIB-4, respectively. CONCLUSION: The metabonomic strategy performed excellently in predicting significant and advanced liver fibrosis in CHC patients, including those in the gray zone of APRI and FIB-4, which may contribute to reducing the need for these patients to undergo liver biopsy.

8.
GED gastroenterol. endosc. dig ; GED gastroenterol. endosc. dig;35(2): 70-73, abr.-jun. 2016. ilus
Artículo en Portugués | LILACS | ID: biblio-1038

RESUMEN

O hepatocarcinoma fibrolamelar (HCC-FL), variante do hepatocarcinoma (HCC), é uma neoplasia rara, responsável por 0,6-8,6% das neoplasias de origem no hepatócito. O diagnóstico é feito através de exames de imagem e confirmado pelo exame anatomopatológico. O transplante de fígado (TF) apresenta-se como tratamento curativo do HCC-FL. Neste relato, documentou-se um caso de hepatocarcinoma fibrolamelar irressecável tratado, de maneira curativa, com o transplante de fígado.


Fibrolamellar hepatocelullar carcinoma (FHCC), a variant of conventional hepatocelullar neoplasms originated in the carcinoma, is a rare neoplasm, responsible for 0.8-8.6% of all hepatocarcinomas. Diagnose is performed through image scans and confirmed through the anatomopatological examination. Liver ressection and liver transplantation are shown as a curative treatment for the FHCC. In this report, we documented a case of unresectable fibrolamellar hepatocellular carcinoma curatively treated with liver transplantation.


Asunto(s)
Humanos , Masculino , Adulto , Trasplante de Hígado , Carcinoma Hepatocelular , Neoplasias Hepáticas
9.
GED gastroenterol. endosc. dig ; GED gastroenterol. endosc. dig;34(3): 123-127, jul.-set. 2015. ilus
Artículo en Portugués | LILACS | ID: lil-779344

RESUMEN

A hepatite autoimune é uma doença de curso crônico e etiologia ainda não esclarecida, que em alguns casos evolui para falência hepática aguda, condição em que o tratamento definitivo é o transplante hepático. Assumidos todos os riscos da cirurgia, restam ainda outras variáveis que concorrem para o êxito do tratamento, como a influência do sistema imune. Dentre essas, uma alteração na formação da lectina ligadora de manose (MBL), presente no fígado do doador, está relacionada a uma maior predisposição do receptor a eventos infecciosos. Relata-se o caso de uma paciente de 18 anos, submetida ao transplante hepático por falência hepática aguda, que, no pós-operatório, apresentou choque séptico de origem desconhecida, evoluindo a óbito. O doador foi genotipado com polimorfismo da MBL relacionado a níveis intermediários ou baixos de MBL sérica. Complicações infecciosas pós-transplantes continuam sendo frequentes nos receptores de órgãos. Assim, a utilização de parâmetros modificadores de resposta imune, como a MBL, poderá ajudar a melhorar o prognóstico desses pacientes.


Autoimmune hepatitisis mostly a chronic disease, which etiology remains unclear, progressing in some cases to acute liver failure, a condition whose definitive treatmentis liver transplantation. Assume dallrisks of surgery, there are still other variables that contribute to the success of the treatment, as the influence of the immune system. Among these, a change in the formation of mannose bindin glectin (MBL), present in the do nor liver, is related to increased predisposition to infectious events in the receptor. It is reported a case of a 18 year old patient who under went liver transplantation for acute liver failure, which evolved post operatively to septics hockofunk now no riginand death. The liver donor was genotyped with polymorphis mof MBL, related to intermediate or low level sof sérum MBL. Post-operative infectious complications remain common in organ recipients. Thus, the use of modified parameters in immune response, such as MBL, may help to improve the prognosis of these patients.


Asunto(s)
Humanos , Femenino , Adolescente , Trasplante de Hígado , Lectina de Unión a Manosa , Fallo Hepático Agudo , Hepatitis Autoinmune
10.
Arq Gastroenterol ; 50(2): 153-6, 2013 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-23903627

RESUMEN

CONTEXT: Data on vascular alterations in patients with hepatosplenic schistosomiasis and portal hypertensive colopathy and changes in these after surgery to decrease portal hypertension are limited. OBJECTIVE: The purpose of this study was to analyse the alterations of portal hypertensive colopathy previously and 6-12 months after splenectomy and gastric devascularization. METHODS: Twelve patients with hepatosplenic schistosomiasis who also had upper gastrointestinal bleeding were studied prospectively. Their endoscopic findings before and 6-12 months after the surgery were analysed. In addition, mucosal biopsies from ascending colon, sigmoid colon and rectum at these time points were subjected to histological and histomorphometric assessment. It was used a control group due to lack of normal pattern of the histomorphometric measures of vessels in individuals without portal hypertension. The critical level of significance adopted in all tests was of a maximum probability error of 5%. RESULTS: Surgery did not lead to significant improvement in histological and endoscopic findings. However, on histomorphometry, there was a significant decrease in the area, diameter and thickness of the vessels in mucosa at all colonic sites. CONCLUSION: Surgery for decompression of schistosomal portal hypertension has a beneficial effect on the associated colopathy, being best indicated in patients with gastrointestinal bleeding and esophageal varices.


Asunto(s)
Enfermedades del Colon/parasitología , Várices Esofágicas y Gástricas/parasitología , Hemorragia Gastrointestinal/parasitología , Hipertensión Portal/parasitología , Esquistosomiasis mansoni/complicaciones , Enfermedades del Colon/cirugía , Colonoscopía , Várices Esofágicas y Gástricas/cirugía , Hemorragia Gastrointestinal/cirugía , Humanos , Hipertensión Portal/cirugía , Estudios Prospectivos , Esquistosomiasis mansoni/cirugía , Índice de Severidad de la Enfermedad , Esplenectomía , Resultado del Tratamiento
11.
Arq. gastroenterol ; Arq. gastroenterol;50(2): 153-156, abr. 2013. tab, graf
Artículo en Inglés | LILACS | ID: lil-679160

RESUMEN

Context Data on vascular alterations in patients with hepatosplenic schistosomiasis and portal hypertensive colopathy and changes in these after surgery to decrease portal hypertension are limited. Objective The purpose of this study was to analyse the alterations of portal hypertensive colopathy previously and 6-12 months after splenectomy and gastric devascularization. Methods Twelve patients with hepatosplenic schistosomiasis who also had upper gastrointestinal bleeding were studied prospectively. Their endoscopic findings before and 6-12 months after the surgery were analysed. In addition, mucosal biopsies from ascending colon, sigmoid colon and rectum at these time points were subjected to histological and histomorphometric assessment. It was used a control group due to lack of normal pattern of the histomorphometric measures of vessels in individuals without portal hypertension. The critical level of significance adopted in all tests was of a maximum probability error of 5%. Results Surgery did not lead to significant improvement in histological and endoscopic findings. However, on histomorphometry, there was a significant decrease in the area, diameter and thickness of the vessels in mucosa at all colonic sites. Conclusion Surgery for decompression of schistosomal portal hypertension has a beneficial effect on the associated colopathy, being best indicated in patients with gastrointestinal bleeding and esophageal varices. .


Contexto Dados em relação às alterações vasculares em pacientes com esquistossomose hepatoesplênica e colopatia hipertensiva portal e suas modificações após cirurgia para atenuação da hipertensão portal são restritos. Objetivo Analisar as alterações da colopatia hipertensiva portal antes e seis a 12 meses após a esplenectomia e desvascularização gástrica. Métodos Foram estudados prospectivamente 12 pacientes com esquistossomose hepatoesplênica e antecedente de hemorragia digestiva alta. Os achados colonoscópicos antes e após 6 a 12 meses após a cirurgia foram analisados. Nesses períodos, biopsias da mucosa do cólon ascendente, sigmóide e reto foram encaminhadas para análise histológica e histomorfométrica. Foi utilizado um grupo controle pela falta de padrão de normalidade das medidas histomorfométricas das vênulas do cólon e reto em indivíduos sem hipertensão portal. O nível de significância crítica adotado em todos os testes foi de probabilidade máxima de erro de 5%. Resultados Não foram encontradas diferenças significantes na intensidade das alterações endoscópicas e histológicas nos vasos da mucosa do cólon e reto após a cirurgia. Entretanto, houve decréscimo estatisticamente significante nas áreas, diâmetros e espessuras dos vasos estudados através da histomorfometria. Conclusão Cirurgia para descompressão da hipertensão portal esquistossomótica tem efeito benéfico na colopatia associada, sendo bem indicada nos pacientes com hemorragia digestive alta e varizes esofágicas. .


Asunto(s)
Humanos , Enfermedades del Colon/parasitología , Várices Esofágicas y Gástricas/parasitología , Hemorragia Gastrointestinal/parasitología , Hipertensión Portal/parasitología , Esquistosomiasis mansoni/complicaciones , Colonoscopía , Enfermedades del Colon/cirugía , Várices Esofágicas y Gástricas/cirugía , Hemorragia Gastrointestinal/cirugía , Hipertensión Portal/cirugía , Estudios Prospectivos , Índice de Severidad de la Enfermedad , Esplenectomía , Esquistosomiasis mansoni/cirugía , Resultado del Tratamiento
12.
Acta Trop ; 126(3): 240-3, 2013 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-23435257

RESUMEN

Upper digestive bleeding is one of the most serious complications of mansoni schistosomiasis, and portal congestive gastropathy (PCG) is responsible for 25-30% of the cases of bleeding instead of bleeding due to esophageal varices. This study aimed to investigate the association between PCG with parameters of portal hypertension and the intensity of periportal fibrosis assessed by ultrasonography, in patients with mansoni schistosomiasis. A prospective study was made of 71 patients whether or not they had a history of upper digestive bleeding, and who had not been previously treated for portal hypertension (splenectomy, use of beta blockers or endoscopic treatment). Patients with other liver diseases were excluded. After signing a form of consent, the patients underwent endoscopy, as well as ultrasonography of the abdomen, and hematological, biochemical and viral markers tests. Chi-square and Fischer's exact tests were used in the statistical analysis. The mean age of the 71 patients was 50 ± 14.5 years of whom 59.2% were women. 45.1% had antecedents of upper digestive bleeding. PCG was observed in 39 patients (54.9%): severe in 8.5%, and mild in 46.5%. A positive association was observed between PCG and the grade of esophageal varices (p=0.017); and the pattern of periportal fibrosis (p=0.041). A negative association was observed between PCG and red spots on the varices (p=0.024). PCG in patients with mansoni schistosomiasis not submitted to treatment for portal hypertension is associated with the sonographic pattern of hepatic fibrosis, as well as with the grade of esophageal varices.


Asunto(s)
Hemorragia Gastrointestinal/epidemiología , Cirrosis Hepática/patología , Esquistosomiasis mansoni/patología , Gastropatías/patología , Adulto , Anciano , Várices Esofágicas y Gástricas/epidemiología , Várices Esofágicas y Gástricas/patología , Femenino , Hemorragia Gastrointestinal/etiología , Humanos , Hipertensión Portal/complicaciones , Cirrosis Hepática/complicaciones , Cirrosis Hepática/parasitología , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Gastropatías/complicaciones , Gastropatías/parasitología
13.
Mem Inst Oswaldo Cruz ; 105(4): 460-6, 2010 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-20721491

RESUMEN

Liver biopsy is the gold-standard method to stage fibrosis; however, it is an invasive procedure and is potentially dangerous. The main objective of this study was to evaluate biological markers, such as cytokines IL-13, IFN-gamma, TNF-alpha and TGF-beta, platelets, bilirubins (Bil), alanine aminotransferase (ALT) and aspartate aminotransferase (AST), total proteins, gamma-glutamil transferase (gamma-GT) and alkaline phosphatase (AP), that could be used to predict the severity of hepatic fibrosis in schistosomiasis and hepatitis C (HC) as isolated diseases or co-infections. The following patient groups were selected: HC (n = 39), HC/hepatosplenic schistosomiasis (HSS) (n = 19), HSS (n = 22) and a control group (n = 13). ANOVA and ROC curves were used for statistical analysis. P < 0.05 was considered significant. With HC patients we showed that TNF-alpha (p = 0.020) and AP (p = 0.005) could differentiate mild and severe fibrosis. With regard to necroinflammatory activity, AST (p = 0.002), gamma-GT (p = 0.034) and AP (p = 0.001) were the best markers to differentiate mild and severe activity. In HC + HSS patients, total Bil (p = 0.008) was capable of differentiating between mild and severe fibrosis. In conclusion, our study was able to suggest biological markers that are non-invasive candidates to evaluate fibrosis and necroinflammatory activity in HC and HC + HSS.


Asunto(s)
Biomarcadores/sangre , Hepatitis C/sangre , Cirrosis Hepática/sangre , Parasitosis Hepáticas/sangre , Esquistosomiasis/sangre , Enfermedades del Bazo/sangre , Adolescente , Adulto , Anciano , Análisis de Varianza , Estudios de Casos y Controles , Hepatitis C/complicaciones , Hepatitis C/patología , Humanos , Cirrosis Hepática/complicaciones , Cirrosis Hepática/patología , Parasitosis Hepáticas/complicaciones , Parasitosis Hepáticas/patología , Persona de Mediana Edad , Necrosis/patología , Curva ROC , Esquistosomiasis/complicaciones , Esquistosomiasis/patología , Índice de Severidad de la Enfermedad , Enfermedades del Bazo/complicaciones , Enfermedades del Bazo/patología , Adulto Joven
14.
Mem. Inst. Oswaldo Cruz ; 105(4): 460-466, July 2010. tab
Artículo en Inglés | LILACS | ID: lil-554813

RESUMEN

Liver biopsy is the gold-standard method to stage fibrosis; however, it is an invasive procedure and is potentially dangerous. The main objective of this study was to evaluate biological markers, such as cytokines IL-13, IFN-ã, TNF-á and TGF-â, platelets, bilirubins (Bil), alanine aminotransferase (ALT) and aspartate aminotransferase (AST), total proteins, ã-glutamil transferase (ã-GT) and alkaline phosphatase (AP), that could be used to predict the severity of hepatic fibrosis in schistosomiasis and hepatitis C (HC) as isolated diseases or co-infections. The following patient groups were selected: HC (n = 39), HC/hepatosplenic schistosomiasis (HSS) (n = 19), HSS (n = 22) and a control group (n = 13). ANOVA and ROC curves were used for statistical analysis. P < 0.05 was considered significant. With HC patients we showed that TNF-á (p = 0.020) and AP (p = 0.005) could differentiate mild and severe fibrosis. With regard to necroinflammatory activity, AST (p = 0.002), ã-GT (p = 0.034) and AP (p = 0.001) were the best markers to differentiate mild and severe activity. In HC + HSS patients, total Bil (p = 0.008) was capable of differentiating between mild and severe fibrosis. In conclusion, our study was able to suggest biological markers that are non-invasive candidates to evaluate fibrosis and necroinflammatory activity in HC and HC + HSS.


Asunto(s)
Adolescente , Adulto , Anciano , Humanos , Persona de Mediana Edad , Adulto Joven , Biomarcadores/sangre , Hepatitis C/sangre , Cirrosis Hepática/sangre , Parasitosis Hepáticas/sangre , Esquistosomiasis/sangre , Enfermedades del Bazo/sangre , Análisis de Varianza , Estudios de Casos y Controles , Hepatitis C , Hepatitis C/patología , Cirrosis Hepática , Cirrosis Hepática/patología , Parasitosis Hepáticas , Parasitosis Hepáticas/patología , Necrosis/patología , Curva ROC , Índice de Severidad de la Enfermedad , Esquistosomiasis , Esquistosomiasis/patología , Enfermedades del Bazo , Enfermedades del Bazo/patología
15.
Arq Gastroenterol ; 46(3): 209-13, 2009.
Artículo en Inglés | MEDLINE | ID: mdl-19918688

RESUMEN

CONTEXT: It still remains an open debate whether Helicobacter pylori eradication is beneficial or not for the improvement of symptoms in functional dyspepsia. Differences in geographic distribution, the worldwide H. pylori genetic variability and the fact that the outcome of infection is strongly related to the virulence of the infecting strain are factors that might be driving ongoing controversies. OBJECTIVE: To study the correlation between gastric histology and H. pylori serology status in patients with dyspepsia. METHODS: This is a cross-sectional study where 40 consecutive dyspeptic patients (28 women and 12 men, mean age 48.5 years) with endoscopically normal stomachs were selected from the endoscopy unit at a university hospital in Recife, PE, Northeast of Brazil, between March 1998 and July 1999. Patients underwent gastric mucosal biopsy and serological tests (anti-Hp and anti-CagA antibodies). Gastric biopsies were examined using H-E and Giemsa stains and gastritis was classified and graded (mild, moderate or severe) according to 'the updated Sydney System-Houston, 1994'. RESULTS: Among 40 patients with dyspepsia the gastric histology revealed that about (1/4) had moderate (25%) or severe (2.5%) gastritis. This subgroup of patients also had a greater positive frequency of anti-Hp (100% vs 41%; P = 0.0005) and anti-CagA (91% vs 58%; P = 0.09) antibodies when compared with those with normal histology (27.5%) or mild gastritis (45%). CONCLUSION: Since upper gastrointestinal endoscopy is part of the functional dyspepsia investigation and serology for anti-CagA antibody is not available in daily clinical practice, by biopsying gastric mucosa we would only be able to selectively apply H. pylori eradication therapy for those with histology that best correlate with virulent infecting strains (moderate or severe gastritis)--around (1/4) of our study patients with dyspepsia.


Asunto(s)
Anticuerpos Antibacterianos/sangre , Antígenos Bacterianos/inmunología , Proteínas Bacterianas/inmunología , Dispepsia/patología , Gastritis/patología , Infecciones por Helicobacter/diagnóstico , Helicobacter pylori/inmunología , Adulto , Anciano , Anciano de 80 o más Años , Antibacterianos/uso terapéutico , Biopsia , Enfermedad Crónica , Estudios Transversales , Toma de Decisiones , Dispepsia/etiología , Dispepsia/microbiología , Femenino , Gastritis/complicaciones , Gastritis/microbiología , Gastroscopía , Infecciones por Helicobacter/tratamiento farmacológico , Helicobacter pylori/patogenicidad , Humanos , Masculino , Persona de Mediana Edad , Índice de Severidad de la Enfermedad , Adulto Joven
16.
Arq. gastroenterol ; Arq. gastroenterol;46(3): 209-213, jul.-set. 2009. graf, tab
Artículo en Inglés | LILACS | ID: lil-530060

RESUMEN

CONTEXT: It still remains an open debate whether Helicobacter pylori eradication is beneficial or not for the improvement of symptoms in functional dyspepsia. Differences in geographic distribution, the worldwide H. pylori genetic variability and the fact that the outcome of infection is strongly related to the virulence of the infecting strain are factors that might be driving ongoing controversies. OBJECTIVE: To study the correlation between gastric histology and H. pylori serology status in patients with dyspepsia. METHODS: This is a cross-sectional study where 40 consecutive dyspeptic patients (28 women and 12 men, mean age 48.5 years) with endoscopically normal stomachs were selected from the endoscopy unit at a university hospital in Recife, PE, Northeast of Brazil, between March 1998 and July 1999. Patients underwent gastric mucosal biopsy and serological tests (anti-Hp and anti-CagA antibodies). Gastric biopsies were examined using H-E and Giemsa stains and gastritis was classified and graded (mild, moderate or severe) according to "the updated Sydney System - Houston, 1994". RESULTS: Among 40 patients with dyspepsia the gastric histology revealed that about » had moderate (25 percent) or severe (2.5 percent) gastritis. This subgroup of patients also had a greater positive frequency of anti-Hp (100 percent vs 41 percent; P = 0.0005) and anti-CagA (91 percent vs 58 percent; P = 0.09) antibodies when compared with those with normal histology (27.5 percent) or mild gastritis (45 percent). CONCLUSION: Since upper gastrointestinal endoscopy is part of the functional dyspepsia investigation and serology for anti-CagA antibody is not available in daily clinical practice, by biopsying gastric mucosa we would only be able to selectively apply H. pylori eradication therapy for those with histology that best correlate with virulent infecting strains (moderate or severe gastritis) - around » of our study patients with dyspepsia.


CONTEXTO: O benefício da terapia de erradicação do H. pylori como parte do tratamento da dispepsia funcional ainda é uma questão em aberto. Diferenças na distribuição geográfica, a ampla variabilidade genética e o fato de que a expressão clínica da infecção está fortemente relacionada com a virulência das cepas infectantes, são fatores que provavelmente guiam as controvérsias. OBJETIVO: Estudar a correlação entre histologia gástrica e sorologia para H. pylori em doentes com dispepsia. MÉTODOS: Estudo descritivo-transversal com 40 pacientes consecutivos com sintomas dispépticos (28 mulheres e 12 homens, média de idade de 48,5 anos) e achado endoscópico de estômago normal, selecionados a partir da sala de endoscopia (Hospital das Clínicas da Universidade Federal de Pernambuco, Recife, PE.) entre março de 1998 e julho de 1999. Todos foram submetidos a biopsias gástricas e testes sorológicos (anti-Hp e anti-CagA). As biopsias foram analisadas pelos métodos de H-E e Giemsa e os achados de gastrite classificados de acordo com o Sistema Sydney atualizado. RESULTADOS: A histologia dos 40 pacientes revelou que cerca de » apresentava gastrite moderada (25 por cento) ou severa (2,5 por cento). Esse grupo também apresentava maior frequência de positividade anti-Hp (100 por cento vs 41 por cento; P = 0,0005) e anti-CagA (91 por cento vs 58 por cento; P = 0,09) quando comparado com os casos com histologia normal (27,5 por cento) ou gastrite leve (45 por cento). CONCLUSÃO: Considerando que a endoscopia digestiva alta é parte da rotina de investigação da dispepsia funcional e que a sorologia anti-CagA não está disponível na prática clínica diária, através da histologia pode-se selecionar e aplicar terapia de erradicação do H. pylori apenas para os casos que muito provavelmente estão associados a cepas patogênicas de H. pylori (doentes com gastrite moderada ou severa) - cerca de » da presente amostra.


Asunto(s)
Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Anticuerpos Antibacterianos/sangre , Antígenos Bacterianos/inmunología , Proteínas Bacterianas/inmunología , Dispepsia/patología , Gastritis/patología , Infecciones por Helicobacter/diagnóstico , Helicobacter pylori/inmunología , Antibacterianos/uso terapéutico , Biopsia , Enfermedad Crónica , Estudios Transversales , Toma de Decisiones , Dispepsia/etiología , Dispepsia/microbiología , Gastroscopía , Gastritis/complicaciones , Gastritis/microbiología , Infecciones por Helicobacter/tratamiento farmacológico , Helicobacter pylori/patogenicidad , Índice de Severidad de la Enfermedad , Adulto Joven
17.
Mem. Inst. Oswaldo Cruz ; 101(supl.1): 353-354, Oct. 2006. graf
Artículo en Inglés | LILACS | ID: lil-441273

RESUMEN

Evaluation of hepatic fibrosis is usually performed by histopathological examination of biopsies. However, this is an invasive and potentially dangerous procedure. Several studies have proposed serum biological markers of hepatic fibrosis. This communication evaluates the use of serum cytokines as markers of hepatic fibrosis in hepatitis C, schistosomiasis, and co-infection.


Asunto(s)
Adulto , Humanos , Citocinas/sangre , Hepatitis C/inmunología , Cirrosis Hepática/parasitología , Esquistosomiasis/inmunología , Biomarcadores/sangre , Citocinas/inmunología , Ensayo de Inmunoadsorción Enzimática , Hepatitis C/complicaciones , /sangre , Cirrosis Hepática/inmunología , Valor Predictivo de las Pruebas , Reproducibilidad de los Resultados , Esquistosomiasis/complicaciones , Factor de Crecimiento Transformador beta/sangre , Factor de Necrosis Tumoral alfa/sangre
18.
Mem Inst Oswaldo Cruz ; 101 Suppl 1: 353-4, 2006 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-17308796

RESUMEN

Evaluation of hepatic fibrosis is usually performed by histopathological examination of biopsies. However, this is an invasive and potentially dangerous procedure. Several studies have proposed serum biological markers of hepatic fibrosis. This communication evaluates the use of serum cytokines as markers of hepatic fibrosis in hepatitis C, schistosomiasis, and co-infection.


Asunto(s)
Citocinas/sangre , Hepatitis C/inmunología , Cirrosis Hepática/parasitología , Esquistosomiasis/inmunología , Adulto , Biomarcadores/sangre , Citocinas/inmunología , Ensayo de Inmunoadsorción Enzimática , Hepatitis C/complicaciones , Humanos , Interleucina-13/sangre , Cirrosis Hepática/inmunología , Valor Predictivo de las Pruebas , Reproducibilidad de los Resultados , Esquistosomiasis/complicaciones , Factor de Crecimiento Transformador beta/sangre , Factor de Necrosis Tumoral alfa/sangre
19.
Mem Inst Oswaldo Cruz ; 99(5 Suppl 1): 67-71, 2004.
Artículo en Inglés | MEDLINE | ID: mdl-15486638

RESUMEN

Portal hypertension is a frequent complication of chronic liver disease, detected not only in schistosomiasis, but also in cirrhosis of any etiology. Vascular alterations in the colonic mucosa are a potential source for acute or chronic bleeding and have been observed in patients with portal hypertension. The purpose of this prospective study was to describe and propose a classification for the vascular alterations of portal hypertension in the colonic mucosa among patients with hepatosplenic schistosomiasis mansoni. One or more alterations of portal colopathy were observed in all patients and they were classified according to their intensity, obeying the classification proposed by the authors. Portal colopathy is an important finding in hepatosplenic schistosomiasis and might be the cause of lower gastrointestinal bleeding in patients with severe portal hypertension.


Asunto(s)
Enfermedades del Colon/parasitología , Hipertensión Portal/parasitología , Parasitosis Hepáticas/complicaciones , Esquistosomiasis mansoni/complicaciones , Enfermedades del Bazo/parasitología , Adulto , Anciano , Enfermedades del Colon/patología , Endoscopía Gastrointestinal , Femenino , Humanos , Hipertensión Portal/patología , Mucosa Intestinal/irrigación sanguínea , Mucosa Intestinal/patología , Parasitosis Hepáticas/patología , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Índice de Severidad de la Enfermedad , Enfermedades del Bazo/patología
20.
Mem. Inst. Oswaldo Cruz ; 99(5,supl.1): 67-71, Aug. 2004. ilus, tab
Artículo en Inglés | LILACS | ID: lil-384482

RESUMEN

Portal hypertension is a frequent complication of chronic liver disease, detected not only in schistosomiasis, but also in cirrhosis of any etiology. Vascular alterations in the colonic mucosa are a potential source for acute or chronic bleeding and have been observed in patients with portal hypertension. The purpose of this prospective study was to describe and propose a classification for the vascular alterations of portal hypertension in the colonic mucosa among patients with hepatosplenic schistosomiasis mansoni. One or more alterations of portal colopathy were observed in all patients and they were classified according to their intensity, obeying the classification proposed by the authors. Portal colopathy is an important finding in hepatosplenic schistosomiasis and might be the cause of lower gastrointestinal bleeding in patients with severe portal hypertension.


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Enfermedades del Colon , Hipertensión Portal , Parasitosis Hepáticas , Esquistosomiasis mansoni , Enfermedades del Bazo , Endoscopía Gastrointestinal , Mucosa Intestinal , Estudios Prospectivos , Índice de Severidad de la Enfermedad
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