RESUMEN

The recent publication of representative genomic sequences of GBV-C has permitted the selection of PCR primers for detection of GBV-C in clinical samples by PCR techniques. Traditional amplification methodologies which couple reverse transcription polymerase chain reaction (RT-PCR) and Southern blot detection are slow, cumbersome, and can be technique dependent. This has hampered studies to determine the clinical significance of GBV-C. We report the selection of highly conserved PCR primers and a probe useful for semi-automated RT-PCR using the Abbott LCx system. This adaptation of the LCx system expands its capabilities to include the detection of RNA by RT-PCR, in addition to DNA detection by ligase chain reaction (LCR).

Asunto(s)

Flaviviridae/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , ARN Viral/sangre , Automatización , Southern Blotting , Cartilla de ADN , Sondas de ADN , Contaminación de Equipos , Flaviviridae/genética , Hepatitis Viral Humana/epidemiología , Humanos , Técnicas para Inmunoenzimas , Prevalencia , ARN Viral/genética , Reproducibilidad de los Resultados , Tamaño de la Muestra , Sensibilidad y Especificidad