RESUMEN
In recent past, the respiratory infection has emerged as a great challenge to the poultry farmers. Various pathogens including Avian pneumovirus (APV), Avian influenza virus (AIV), Infectious bronchitis virus (IBV) and Newcastle disease virus (NDV), Avibacterium paragallinarum, Ornithobacterium rhinotracheale (ORT), Mycoplasma synoviae (MS), Mycoplasma gallisepticum (MG) and Avian pathogenic Escherichia coli (APEC) are involved in the respiratory disease complex in birds [1], [2] (Bradbury, 1984; Roussan et al., 2008). Hence, respiratory disease complex is the most serious disease affecting to poultry and causes heavy economic losses in the poultry industry worldwide [3] (Murthy et al., 2008). In recent years, metagenomics is powerful analyzing tool for detection of pathogens directly from clinical samples without any prior knowledge of the organism in a given sample [4], [5] (Schuster, 2008; Pereira et al., 2010). High throughput Next-Generation-Sequencing technology was used for sequencing the isolated genomic DNA. These data provides an insight about taxonomic and functional status of microorganisms responsible for causing respiratory infection in broiler. The data of these metagenome are available in the BioSample Submission Portal as Bioproject PRJNA339659 and SRA accession number SRR5997823, SRR5992854, SRR6037376, SRR6024702, SRR6012248 and SRR6008913.
RESUMEN
One hundred and eighteen samples of uncooked pork sausages were analysed bacteriologically. The total aerobic plate count ranged from 1.8 x 10(8) to 9.2 x 10(8) CFU/g in the samples. High mean counts of Staphylococci, Enterococci, Micrococci, Bacillus cereus, Salmonella and Coliforms were observed. Staphylococcus aureus were encountered with count range 2.8 x 10(6) CFU/g to 7.4 x 10(6) CFU/g. The viable count of Escherichia coli ranged from 2.0 x 10(5) to 4.3 x 10(5) CFU/g. Clostridium perfringens were detected in 66.9% of samples with a count ranging from 3.9 x 10(2) to 1.1 x 10(7) Cl. perfringens/g. None of 118 samples contained Yersinia enterocolitica.
Asunto(s)
Bacterias/aislamiento & purificación , Microbiología de Alimentos , Carne , Animales , PorcinosRESUMEN
Serum samples from 1134 vaccinated and non vaccinated animals (775 cattle and 359 buffaloes) were screened with different serological tests like Rose Bengal plate Test (RBPT), plate agglutination test (PAT) and standard tube agglutination test (SAT). The agreement between these three screening tests was 87.19% and 97.86% in vaccinated and non vaccinated cattle while corresponding figures in buffaloes were 91.56% and 84.42%. Among vaccinated animals a positive percentage of 12.8 and 8.8 was noticed in cattle was 4.7 and 6.3, respectively by RBPT. All the positive sera samples were further tested by mercaptoethanol test (MET) and heat inactivation test (HIT) to conform the result of screening tests and to differentiate vaccinal titre from that of active infection. It was concluded that RBPT is overall best screening test to diagnose bovine brucellosis. MET and HIT are good supplementary tests but MET is better than HIT both in cattle and buffaloes. Some high titered non vaccinated buffaloes were negative in RBPT but positive by all other tests.