RESUMEN

Methanolic extract of Phyllanthus amarus was tested for its anti-mutagenic activity in Salmonella typhimurium strains TA1535, TA100, and TA102 (Ames test). P. amarus extract was able to inhibit the activation and mutagenicity of 2-acetaminofluorene (2-AAF) and aflatoxinB(1) at concentrations of 0.25-2 mg/plate. It was also found to inhibit mutagenicity induced by direct acting mutagens sodium azide (NaN(3)), N-methyl-N-nitro-N-nitrosoguanidine (MNNG), and 4-nitro-0-phenylenediamine (NPD), at concentrations of 1 mg to 0.25 mg/plate. Urinary mutagenicity produced in rats by benzo[a] pyrene was found to be significantly inhibited by the oral administration of Phyllanthus extract. These results indicate significant anti-mutagenicity of the extract in vitro as well as in vivo.

Asunto(s)

Antimutagênicos/farmacología , Phyllanthus/química , Extractos Vegetales/farmacología , Salmonella typhimurium/efectos de los fármacos , 2-Acetilaminofluoreno/antagonistas & inhibidores , 2-Acetilaminofluoreno/farmacología , Aflatoxina B1/antagonistas & inhibidores , Aflatoxina B1/farmacología , Animales , Antimutagênicos/administración & dosificación , Benzo(a)pireno/farmacocinética , Biotransformación/efectos de los fármacos , Evaluación Preclínica de Medicamentos , Técnicas In Vitro , Masculino , Metanol , Metilnitronitrosoguanidina/farmacología , Fenilendiaminas/antagonistas & inhibidores , Fenilendiaminas/farmacología , Extractos Vegetales/administración & dosificación , Hojas de la Planta/química , Tallos de la Planta/química , Ratas , Ratas Wistar , Salmonella typhimurium/genética , Azida Sódica/antagonistas & inhibidores , Azida Sódica/farmacología , Solventes , Orina/química