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Molecules ; 25(6)2020 Mar 18.
Artículo en Inglés | MEDLINE | ID: mdl-32197377

RESUMEN

In this study, we isolated from the aerial parts of Baccharis conferta Kunth (i) a new neoclerodane, denominated "bacchofertone"; (ii) four known terpenes: schensianol A, bacchofertin, kingidiol and oleanolic acid; and (iii) two flavonoids: cirsimaritin and hispidulin. All structures were identified by an exhaustive analysis of nuclear magnetic resonance (NMR) and mass spectroscopy (MS). Extracts from aerial parts were screened for anti-inflammatory activity in the mice ear edema model of 12-O-tetradecanoylforbol-13-acetate mice. Dichloromethane extract (BcD) exhibited 78.5 ± 0.72% inhibition of edema, followed by the BcD2 and BcD3 fractions of 71.4% and 82.9% respectively, at a dose of 1 mg/ear. Kingidiol and cirsimaritin were the most potent compounds identified, with a median effective dose of 0.12 and 0.16 mg/ear, respectively. A histological analysis showed that the topical application of TPA promoted intense cell infiltration, and this inflammatory parameter was reduced with the topical application of isolated compounds.


Asunto(s)
Antiinflamatorios , Baccharis/química , Enfermedades del Oído , Edema , Flavonas , Terpenos , Acetato de Tetradecanoilforbol/toxicidad , Animales , Antiinflamatorios/química , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/farmacología , Modelos Animales de Enfermedad , Enfermedades del Oído/inducido químicamente , Enfermedades del Oído/tratamiento farmacológico , Enfermedades del Oído/metabolismo , Enfermedades del Oído/patología , Edema/inducido químicamente , Edema/tratamiento farmacológico , Edema/metabolismo , Edema/patología , Flavonas/química , Flavonas/aislamiento & purificación , Flavonas/farmacología , Masculino , Ratones , Ratones Endogámicos ICR , Terpenos/química , Terpenos/aislamiento & purificación , Terpenos/farmacología
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