RESUMEN
A new and rare ergostane triterpenoid, 4alpha-methyl-3alpha,4beta-dihydroxy-ergost-8,24(28)-dien-7,11-dion-25-al (1), was isolated from cultures of the basidiomycete Inocybe lilacina, and its structure established on the basis of spectral methods (MS, IR, 1D and 2D NMR).
Asunto(s)
Basidiomycota/química , Ergosterol/análogos & derivados , Basidiomycota/crecimiento & desarrollo , Ergosterol/aislamiento & purificación , Fermentación , Espectroscopía de Resonancia Magnética , Espectrometría de Masa por Ionización de Electrospray , Espectrofotometría Infrarroja , Espectrofotometría UltravioletaRESUMEN
A new ergostane triterpenoid (4 alpha-methylergost-8,24(28)-dien-3,7,1 1-trione, 1) together with four known compounds (2-5) were isolated from cultures of the spectroscopic and M Sexperiments. basidiomycete Inocybe lilacina. The structure of the new compound was established on the basis of IR, 1D and 2D NMR
Asunto(s)
Basidiomycota/química , Ergosterol/análogos & derivados , Triterpenos/aislamiento & purificación , Ergosterol/química , Ergosterol/aislamiento & purificación , Estructura Molecular , Triterpenos/químicaRESUMEN
Extramedullary hematopoiesis (EMH), defined as the presence of hematopoiesis outside bone marrow and peripheral blood, occurs asa compensatory phenomenon in several hematologic disorders and bone marrow dysfunction. EMH predominantly affects reticuloendothelial system including the spleen, liver and lymph nodes. Here,we report a rare case of multiple intracranial meningeal EMH. A37-year-old woman was anemic with gradually worsening vision for 8 months. Multiple extra-axial masses were found on imaging and the patient underwent the biopsy for the left frontotemporal lesion.Final diagnosis was multiple intracranial meningeal EMH. Treatment of fractionated external beam radiotherapy resulted in marked symptomatic improvement. This case indicates that although the diagnosis of meningeal EMH is difficult, there is a need to consider EMHin the differential diagnosis of anemic patients with tumor-like mass lesions in extramedullary sites.
Asunto(s)
Neoplasias Encefálicas , Traumatismo Múltiple , Adulto , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/terapia , Corteza Cerebral/patología , Femenino , Glicoforinas/metabolismo , Humanos , Integrina beta3/metabolismo , Imagen por Resonancia Magnética , Traumatismo Múltiple/diagnóstico , Traumatismo Múltiple/terapia , Peroxidasa/metabolismoRESUMEN
OBJECTIVE: To explore the effect of low doses X-ray on proliferation of hippocampal pyramidal cell in the area of CA1 in prenatal rat and its relevant mechanism. METHODS: A total of 25 pregnant rats were randomly divided into four experimental groups and one control group. The experimental groups, in a duration of consistent 18 days, respectively received different doses as follows: 0.015 mGy/d, 0.03 mGy/d, 0.06 mGy/d and 0.09 mGy/d. The control group received sham radiation. To observe the density and width of hippocampal pyramidal cell in the area of CA1 by HE stained and observe the expression of the ERK1/2 by IHM. RESULTS: (1) Except C group, all other groups presented increment in width of the level of hippocampal pyramidal cell, compared with C group; H group, M group, L1 group and L2 group were higher than that (F value respectively were 8.475, 33.42, 14.395, 44.955; P value respectively were 0.002, 0.048, 0.030, 0.012). But the phenomenon of inhomogeneity in width in H group was observed, at the same time, the density of cell in H group became looser (F = 4.466, P = 0.017). (2) The expression of ERK1/2 in the hippocampus CA1 was seen in cytoplasm of every group, the average optical density of positive ERK1/2 protein significantly increased in L1 group and L2 group, compared with control group respectively (F value respectively were 4.561, 4.103, P value respectively were 0.044, 0.035). CONCLUSION: Low doses X-ray could promote proliferation of hippocampus CA1 cell in prenatal. The reason could be the increment of the ERK1/2 protein induced by X-ray. When the doses reached 0.09 mGy/d, the excesses proliferation phenomenon was observed.
Asunto(s)
Proliferación Celular/efectos de la radiación , Hipocampo/citología , Exposición Materna , Neuronas/efectos de la radiación , Células Piramidales/efectos de la radiación , Animales , Femenino , Hipocampo/efectos de la radiación , Masculino , Neuronas/citología , Embarazo , Células Piramidales/citología , Radiación Ionizante , Ratas , Rayos XAsunto(s)
Carcinoma/metabolismo , Carcinoma/patología , Neoplasias de la Vesícula Biliar/metabolismo , Neoplasias de la Vesícula Biliar/patología , Proteína Kangai-1/metabolismo , Nucleósido Difosfato Quinasas NM23/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Tomografía Computarizada EspiralRESUMEN
OBJECTIVE: To investigate the expression of two tumor metastasis suppressor genes nm23 and KAI1 in gallbladder adenocarcinoma, and their clinicopathological significance. METHODS: Specimens and clinical data from 31 gallbladder adenocarcinoma patients were collected. Histopathological grading and the expression of nm23 and KAI1 were detected by HE and immunohistochemical staining, respectively. All cases were followed up for at least three years. RESULTS: Immunohistochemical staining showed that the positive rate of nm23 and KAI1 proteins was 71.0% (22/31) and 61.3% (19/31), respectively. The positive expression rates of nm23 and KAI1 proteins in the early stage carcinomas were significantly higher than those in the moderate and advanced stage ones (P exact = 0.0051 and P exact = 0.0084), and both had an negative correlation with clinicopathologic stage (P trend = 0.0047 and P trend = 0.0058). There was a significant difference in the expression of nm23 and KAI1 proteins among well, moderately and poorly differentiated carcinomas (P exact = 0.0328 and P exact = 0.0020). The expression of nm23 and KAI1 was positively correlated with histopathological grade (P trend = 0.0086 and P trend = 0.0006). There was also a significant difference in the expression of nm23 and KAI1 proteins between 17 survival and 14 dead patients (P exact = 0.0038 and P exact = 0.0001 ). A synergistic effect of nm23 and KAI1 protein on the survival was observed , and seemed to be more important than any individual gene alone (P exact = 0.0005). CONCLUSION: The expressions of nm23 and KAI1 proteins are negatively correlated with clinical stage, but positively with histopathological grade in gallbladder adenocarcinoma. These two tumor metastasis suppressor genes may act synergistically to inhibit the tumor metastasis.