RESUMEN
Objective: To investigate the effects of the repair and restitution of ear-shaped cartilage by adipose tissue-derived stem cells(ADSC) and cartilage acellular extracellular matrix. Methods: ADSC were extracted by digesting with collagenase type II from the adipose tissue from 32 patients with adiposity whose fats were drawn, and were cultured and subcultured in vitro. The natural biological scaffolds were prepared by acellular method using porcine ear cartilage, and then the second generation ADSC(5.0×10(7)/ml) were inoculated on the preformed natural bio-scaffold scaffold by culturing in vitro for 3 days to form a cell scaffold complex. 32 New Zealand white rabbits were randomly divided into the experimental groups, the control group A, the control group B and the control group C. All New Zealand white rabbits were modeled by ear cartilage defects. The cell scaffolds composite was implanted into the experimental group of the ear cartilage defects of rabbits, the ADSC were implanted into the control group A, the cartilage acellular extracellular matrix scaffold was implanted into the control group B and the control group C was modeled only by ear cartilage defects. After 16 weeks, the animals were sacrificed and the repair effect was observed by gross appearance and histological examinations including HE, Toluidine blue staining, Safranin O and typeâ ¡ collagen staining. Its were quantitatively analyzed by positive staining results of type â ¡ collagen. Ear cartilage tissue elasticity was detected. SPSS 17.0 software was used to analyze the data. Results: The cartilage defects in the experimental group were repaired well by general shape observation and those in the control group was filled in with granulation tissue. There were significant differences between the experimental group and the control group in the wet weight(P<0.05). HE staining showed that cartilage cavities formed in articular cartilage defects, and only the fibrous tissue was filled with the ear cartilage defect in the control groups. In the repair area, Toluidine blue staining, Safranin O and type â ¡ collagen staining were positive in the experimental group, and negative in the control groups. There was no significant difference between the experimental group and the normal ear cartilage in the ear cartilage elastic constant detection(P>0.05). Conclusions: The mechanics and histology of rabbit ear neonatal cartilage constructed by ADSC combined with cartilage acellular matrix are close to normal ear cartilage. Cartilage acellular matrix material combined with adipose-derived stem cells has good repair and reconstruction ability for ear cartilage defects, which possesses potential clinical application value.
Asunto(s)
Tejido Adiposo/citología , Cartílago Auricular , Deformidades Adquiridas del Oído/terapia , Matriz Extracelular/fisiología , Células Madre/fisiología , Ingeniería de Tejidos , Andamios del Tejido , Animales , Sistema Libre de Células , Células Cultivadas , Cartílago Auricular/anatomía & histología , Conejos , Distribución Aleatoria , Trasplante de Células Madre/métodos , PorcinosRESUMEN
This report describes a case of cryptococcal meningitis as the initial manifestation of acquired immunodeficiency syndrome (AIDS). During the admission, computed tomography (CT) of brain revealed multiple lesions with ring-enhancement over the cerebellum, frontal and temporal lobes. Autopsy findings showed diffuse cryptococcal meningoencephalitis and central nervous system (CNS) lymphoma forming multiple round ring abscesses. The case demonstrated that opportunistic cryptococcal infection and primary CNS lymphoma may coexist in a patient with AIDS.