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PURPOSE: Preeclampsia (PE) is a pregnancy-specific syndrome with increasing maternal and perinatal morbidity and mortality. Succinylation, a post-translational modification event, has been found in various diseases. However, the role of succinylation in PE has not been explored. This study aimed to investigate the effect of succinylation on PE and the underlying mechanisms. METHODS: Thirty-two PE patients and 32 normal pregnancy volunteers were recruited. Human extravasated trophoblast cells (HTR-8/SVneo) were used in in vitro study. RT-qPCR was performed to detect the expression of succinylation-related mRNAs. The cell proliferation, invasion, and migration were assessed using cell counting kit-8, ethynyldeoxyuridine, transwell, and wound healing assays. Co-immunoprecipitation and dual-luciferase reporter assays were performed to analyze the interaction between sirtuin (SIRT)5 and homeobox box 3 (HOXB3). RESULTS: SIRT5 was increased in the placental tissues of PE patients. SIRT5 inhibition increased cell proliferation, invasion, and migration in HTR-8/SVneo cells. Mechanistic investigations indicated that HOXB3 was a downstream regulatory target of SIRT5-mediated desuccinylation. Rescue experiments further verified that silencing of HOXB3 inhibited cell proliferation, invasion, and migration. Additionally, HOXB3 deficiency reversed the activation of the Notch and ß-catenin signaling pathway induced by SIRT5 inhibition. CONCLUSION: SIRT5 inhibited the trophoblast cell proliferation, invasion, and migration to promote PE through suppressing Notch and ß-catenin signaling pathway activation via desuccinylating HOXB3.
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Preeclampsia (PE) harms a significant number of pregnant women and fetuses. However, because of its complex pathological mechanisms, there is no cure except for delivery. This study identified the impact and mechanisms of action of HOXB3 in PE. The behaviors of HTR-8/SVneo cells were analyzed using a cell counting kit-8, EdU, and transwell assays. The interaction between HOXB3 and Notch1 was assessed using a luciferase reporter and chromatin immunoprecipitation assays. Expression was measured by quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence assays. Additionally, the function of HOXB3 was evaluated in an established rat model of PE. We found that HOXB3 was upregulated in PE. HOXB3 overexpression facilitated trophoblast cell proliferation, migration, and invasion. HOXB3 transcriptionally regulated Notch1 by binding to its promoter. Notch1 knockdown abrogated the functions of HOXB3 and the-catenin pathway in trophoblasts. Suppression of the Wnt/ß-catenin pathway abrogated the effects of HOXB3. Additionally, HOXB3 alleviated the symptoms in PE rats. In conclusion, HOXB3 transcriptionally activated Notch1 expression and the-catenin pathway, promoting trophoblast cell proliferation, invasion, and migration, thereby alleviating PE progression. This study provides a novel approach for PE therapy.
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Proteínas de Homeodominio , Preeclampsia , Animales , Femenino , Embarazo , Ratas , beta Catenina/metabolismo , Cateninas/metabolismo , Movimiento Celular , Proliferación Celular , Placenta/metabolismo , Preeclampsia/metabolismo , Trofoblastos/metabolismo , Proteínas de Homeodominio/genética , Proteínas de Homeodominio/metabolismo , Vía de Señalización WntRESUMEN
OBJECTIVE: This study aims to investigate the mechanism by which magnesium sulfate regulates the miR-218-5p/HMGB-pathway-mediated apoptosis of vascular endothelial cells (VECs) in rats with preeclampsia (PE). METHODS: Twenty pregnant rats were randomly divided into four groups: normal, PE, MgSO4, and high-mobility group protein B1 (HMGB1)-agomir groups. On the 14th day of each rat's pregnancy, endotoxin was used to establish a PE model in the PE, MgSO4, and HMGB1-agomir groups. Then, the MgSO4 and HMGB1-agomir groups were treated with magnesium sulfate. Finally, HMGB1 overexpression was performed only in the HMGB1-agomir group. The rats' urinary protein content and systolic blood pressure at 24 h were detected on the 11th, 13th, 15th, 17th, and 19th day of pregnancy. RESULTS: Compared with the PE group, 24-h urinary protein content, blood pressure, VEC apoptosis rate, apoptosis marker levels, and HMGB1 expression decreased while miR-218-5p levels increased in the MgSO4 group. The dual-luciferase assay revealed that HMGB1 can be targeted and regulated by miR-218-5p. Compared with the MgSO4 group, 24-h urinary protein content, blood pressure, VEC apoptosis rate, apoptosis marker levels, and HMGB1 expression increased while miR-218-5p levels decreased in the HMGB1-agomir group. CONCLUSION: MgSO4 reduces VEC apoptosis in PE rats via the miR-218-5p/HMGB1 pathway and thus plays a role in treating PE.
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Apoptosis , Células Endoteliales/citología , Proteína HMGB1 , MicroARNs , Preeclampsia , Animales , Femenino , Proteína HMGB1/genética , Sulfato de Magnesio/farmacología , MicroARNs/genética , Preeclampsia/tratamiento farmacológico , Preeclampsia/genética , Embarazo , RatasRESUMEN
As the global water shortage becomes increasingly serious, it is highly imperative to develop efficient, renewable, and large-scale water purification devices. Herein, an efficient solar-driven water purification device of wood coated with Fe2O3 nanoparticle-decorated carbon nanotubes (Fe2O3/CNT) is fabricated in only a few seconds by one-step combustion of ferric acetylacetonate in an ambient environment. The thin layer of the Fe2O3/CNT hybrid coated on the upper surface of the wood serves as a solar-light absorber for converting solar energy to thermal energy, while the thermally insulating wood layer with vertically aligned channels endows the device with rapid water upward transport and localizes the generated heat inside the Fe2O3/CNT layer for solar-driven water evaporation. As a result, the wood/Fe2O3/CNT device achieves a high water steam generation capability of 1.42 kg m-2 h-1 along with an excellent evaporation efficiency of 87.2% under 1 sun irradiation, higher than most of the wood-based solar-driven water evaporation device reported. This device is also efficient in the purification of seawaters and wastewaters. This work demonstrates a rapid and facile methodology for large-scale fabrication of wood/Fe2O3/CNT devices for efficient solar-driven water purification.
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Real-time, nondestructive, and accurate estimation of plant water status is important to the precision irrigation of winter wheat. The objective of this study was to develop a method to estimate plant water content (PWC) by using canopy spectral proximal sensing data. Two experiments under different water stresses were conducted in 2014-2015 and 2015-2016. The PWC and canopy reflectance of winter wheat were collected at different growth stages (the jointing, booting, heading, flowering, and filling stages in 2015 and the jointing, booting, flowering, and filling stages in 2016). The performance of different spectral transformation approaches was further compared. Based on the optimal pretreatment, partial least squares regression (PLSR) and four combination methods [i.e., PLSR-stepwise regression (SR), PLSR-successive projections algorithm (SPA), PLSR-random frog (RF), and PLSR-uninformative variables elimination (UVE)] were used to extract the sensitive bands of PWC. The results showed that all transformed spectra were closely correlated to PWC. The PLSR models based on the first derivative transformation method exhibited the best performance (coefficient of determination in calibration, R 2 C = 0.96; root mean square error in calibration, RMSEC = 20.49%; ratio of performance to interquartile distance in calibration, RPIQC = 9.19; and coefficient of determination in validation, R 2 V = 0.86; root mean square error in validation, RMSEV = 46.27%; ratio of performance to interquartile distance in validation, RPIQV = 4.34). Among the combination models, the PLSR model established with the sensitive bands from PLSR-RF demonstrated a good performance for calibration and validation (R 2 C = 0.99, RMSEC = 11.53%, and RPIQC = 16.34; and R 2 V = 0.84, RMSEV = 44.40%, and RPIQV = 4.52, respectively). This study provides a theoretical basis and a reference for estimating PWC of winter wheat by using canopy spectral proximal sensing data.
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Excessive neutrophil extracellular trap (NET) formation may contribute to polymyositis (PM)-associated interstitial lung diseases (ILD), but the underlying mechanism is not fully revealed. In this study, we found that NET accelerated the progression of ILD and promoted pulmonary fibrosis (PF) in vivo. miR-7 expression was down-regulated in lung tissue of PM group than control group, and NETs further decreased miR-7 expression. TLR9 and Smad2 were up-regulated in lung tissue of PM group than control group, and NETs further increased TLR9 and Smad2 expressions. In vitro experiments showed that PMA-treated NETs accelerated the proliferation of LF and their differentiation into myofibroblast (MF), whereas DNase I decreased the promotion effect of NETs. Neutrophil extracellular trap components myeloperoxidase (MPO) and histone 3 also promoted the proliferation and differentiation of LF. In addition, we demonstrated that TLR9 involved in the regulation of NETs on LF proliferation and differentiation, and confirmed the interaction between miR-7 and Smad2 in LF. Finally, miR-7-Smad2 pathway was confirmed to be involved in the regulation of TLR9 on LF proliferation and differentiation. Therefore, NETs promote PM-related ILD, and TLR9-miR-7-Smad2 signalling pathway is involved in the proliferation of LFs and their differentiation into MFs.
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Trampas Extracelulares/metabolismo , Fibroblastos/metabolismo , Pulmón/patología , MicroARNs/metabolismo , Polimiositis/patología , Transducción de Señal , Proteína Smad2/metabolismo , Receptor Toll-Like 9/metabolismo , Animales , Secuencia de Bases , Diferenciación Celular , Proliferación Celular , Progresión de la Enfermedad , Femenino , Histonas/metabolismo , Humanos , Ratones Endogámicos BALB C , MicroARNs/genética , Miofibroblastos/patología , Peroxidasa/metabolismo , Ratas Sprague-DawleyRESUMEN
Although versatile piezoresistive pressure sensors show a great potential as human motion detection and wearable smart devices, it is still an issue to widen their working range and enhance their sensitivity. Herein, hollow-structured MXene-polydimethylsiloxane composites (MPCs) are fabricated by utilizing nickel foam as the three-dimensional substrate for dip-coating of MXene sheets followed by infiltrating of polydimethylsiloxane and etching of the nickel foam substrate. The resultant MPC performs a wide working range with bending angles of 0° to 180°, an excellent long-term reliability up to 1000 cycles under the bending angles of 15°, 30° and 150°, and a stable durability with a bending angle of 30° in a frequency range from 0.05 to 2â¯Hz as a bendable piezoresistive pressure sensor, which is attributed to the formation of dense conduction paths due to the interconnection of MXene sheets during the deformation of MPC. The sensor also exhibits an extremely low detection limit of 10â¯mg for pressure detection. Interestingly, the slippage of adjacent MXene sheets is beneficial for monitoring slight vibration of equipments and detecting subtle human motions. Thus, the MPC sensor could be applied for stereo sound and ultrasonic vibration monitoring, swallowing, facial muscle movement, and various intense motion detections, demonstrating its great potential as wearable smart devices.
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Aluminio/química , Carbono/química , Dimetilpolisiloxanos/química , Titanio/química , Tamaño de la Partícula , Porosidad , Propiedades de SuperficieRESUMEN
Rapid and non-destructive estimation of plant water status is essential for adjusting field practices and irrigation schemes of winter wheat. The objective of this study was to find new combination spectral indices based on canopy reflectance for the estimation of plant water status. Two experiments with different irrigation regimes were conducted in 2015-2016 and 2016-2017. The canopy spectra were collected at different growth stages of winter wheat. The raw and derivative reflectance of canopy spectra showed obvious responses to the change of plant water status. Except for equivalent water thickness (EWT), other water metrics had good relationships with new combination spectral indices (R2>0.7). An acceptable model of canopy water content (CWC) was established with the best spectral index (RVI (1605, 1712)). Models of leaf water content (LWC) and plant water content (PWC) had better performances. Optimal spectral index of LWC was FDRVI (687, 531), having R2, RMSE and RPD of 0.77, 2.181 and 2.09; R2, RMSE and RPD of 0.87, 2.652 and 2.34 for calibration and validation, respectively. And PWC could be well estimated with FDDVI (688, 532) (R2, RMSE and RPD of 0.79, 3.136 and 2.21; R2, RMSE and RPD of 0.83, 3.702 and 2.18 for calibration and validation, respectively). Comparing the performances of estimation models, the new combination spectral indices FDRVI (687, 531) based on canopy reflectance improved the accuracy of estimation of plant water status. Besides, based on FDRVI (687, 531), LWC was the optimal water metrics for plant water status estimation.
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Análisis Espectral , Triticum/química , Agua/análisis , Calibración , Hojas de la Planta/química , Estaciones del AñoRESUMEN
The association between inflammatory factor expression and blood pressure with urinary protein in the placenta of pregnant women with pregnancy-induced hypertension (PIH) was investigated to provide a new vision for the clinical prevention and treatment of PIH. Rats were used as animal models and were randomly divided into three groups (control, hypertension and treatment groups) on day 15 of pregnancy with 20 rats in each group. The 10% hypoxia-induced PIH group was induced with administration of an anti-hypertensive drug, and the treatment group was giventreprostinil for one week after the 10% hypoxia-induced PIH. On the 21st day, the experiment was terminated and the placenta was taken to measure the mRNA and protein expression levels of IL-6 and TNF-α, respectively. Pearson's correlation analysis demonstrated the correlation between IL-6 and TNF-α with blood pressure and urinary protein. The blood pressure and urinary protein concentrations in the hypertension group were significantly higher than that in the control group, and the expression levels of IL-6 and TNF-α in the hypertension group were significantly higher (P<0.05). The treatment group significantly reduced inflammatory cytokines and blood pressure and urinary protein levels (P<0.05). Pearson's correlation analysis showed that IL-6 and TNF-α were positively correlated with blood pressure and urinary protein concentration. The blood pressure and urinary protein concentration in PIH rats and the expression levels of IL-6 and TNF-α were significantly higher, and IL-6 and TNF-α were positively correlated with blood pressure and urine protein concentration.
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BACKGROUND: Paracrine signaling of the hepatocyte growth factor (HGF) cytokine plays an important role in survival and invasion ability of placental trophoblasts. However, the intracellular factors and biological pathways underlying these responses remain unclear. METHODS: This study investigated whether HGF affected the expression of homeobox gene HLX1, which is principally expressed in reproductive tissues and in some immune cells, and evaluated the implications of such in the HGF-induced human trophoblast cell line HTR-8/SVneo. RESULTS: HGF was found to up-regulate both HLX1 mRNA and protein levels. Transient transfection of small interfering RNA (siRNA) targeting HLX1 abrogated its induction by HGF. Functionally, HLX1 siRNA not only reduced the growth and invasion capacities of HTR-8/SVneo cells at the basal level, but also inhibited these responses induced by HGF treatment. CONCLUSIONS: HLX1 is an essential downstream signaling component of HGF that leads to growth and invasiveness of trophoblast cells.
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Factor de Crecimiento de Hepatocito/metabolismo , Proteínas de Homeodominio/metabolismo , Factores de Transcripción/metabolismo , Trofoblastos/metabolismo , Regulación hacia Arriba , Western Blotting , Línea Celular , Movimiento Celular , Proliferación Celular , Supervivencia Celular , Femenino , Silenciador del Gen , Proteínas de Homeodominio/antagonistas & inhibidores , Proteínas de Homeodominio/genética , Humanos , Metaloproteinasa 2 de la Matriz/metabolismo , Metaloproteinasa 9 de la Matriz/metabolismo , Placentación , Embarazo , ARN Mensajero/metabolismo , ARN Interferente Pequeño , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Factores de Transcripción/antagonistas & inhibidores , Factores de Transcripción/genética , Trofoblastos/citologíaRESUMEN
OBJECTIVE: To investigate the changes in coagulation and fibrinolysis parameters during acute phase of coronary heart disease, and to explore the effects of drug intervention in same period. METHODS: A prospective study was conducted. The changes in plasma von Willebrand factor (vWF), platelet activating factor (PAF), tissue type plasminogen activator (t-PA) of 110 patients with coronary heart disease [50 patients with acute myocardial infarction (AMI), 35 patients with unstable angina pectoris (UAP), 25 patients with ischemic cardiomyopathy (ICM)] were determined immediately at admission and 14 days after treatment. The indexes of 19 healthy individuals were collected as control. At the same time, randomized and double-blind observation of the changes in plasma coagulation and fibrinolysis system indexes including vWF, PAF and t-PA was made in AMI and UAP patients who received conventional treatment (aspirin + low molecular weight heparin) or combination of conventional treatment and clopidogrel for 14 days. RESULTS: The vWF and PAF levels (nmol/L) of AMI and UAP patients at admission were significantly higher than those in healthy control group [vWF: (202.31 ± 27.38)%, (188.65 ± 31.08)% vs. (120.37 ± 18.79)%; PAF: 50.64 ± 13.25, 48.87 ± 13.24 vs. 15.43 ± 9.27, all P < 0.05], however, t-PA (µg/L) was remarkably lower than that in healthy control group (3.52 ± 1.57, 4.03 ± 2.04 vs. 9.54 ± 1.32, both P < 0.01). After 14 days of treatment, the levels of vWF, PAF, t-PA, fibrinogen (Fib, g/L), D-dimer (mg/L) in plasma of AMI and UAP patients were close to those of healthy control group [vWF: (116.56 ± 26.10)%, (111.28 ± 22.31)% vs. (120.37 ± 18.79)%; PAF: 17.48 ± 9.16, 16.23 ± 9.17 vs. 15.43 ± 9.27; t-PA: 7.91 ± 2.42, 9.01 ± 2.01 vs. 9.54 ± 1.32; Fib: 3.64 ± 0.53, 2.74 ± 0.72 vs. 2.92 ± 0.91; D-dimer: 0.370 ± 0.150, 0.288 ± 0.169 vs. 0.255 ± 0.109, all P>0.05]. However, there was no statistical difference in vWF, PAF, t-PA, Fib and D-dimer levels before and after treatment in ICM group compared with healthy control group [vWF: (124.14 ± 21.17)%, (119.44 ± 26.28)% vs. (120.37 ± 18.79)%; PAF: 15.69 ± 7.14, 14.84 ± 9.16 vs. 15.43 ± 9.27; t-PA: 8.62 ± 2.24, 8.07 ± 2.51 vs. 9.54 ± 1.32; Fib: 3.24 ± 0.74, 3.04 ± 0.77 vs. 2.92 ± 0.91; D-dimer: 0.257 ± 0.132, 0.268 ± 0.117 vs. 0.255 ± 0.109, all P>0.05]. Multiple linear regression analysis showed that, in patients with coronary heart disease, PAF be positively correlated with vWF (r = 0.42, P < 0.01), but negatively correlated with t-PA (r = -0.31, P < 0.01). In patients with AMI and UAP, using the treatment of clopidogrel for 14 days, the levels of vWF, PAF, t-PA, Fib and D-dimer in plasma showed no significant differences compared with those of conventional treatment group [vWF: (120.16 ± 16.57)% vs. (118.12 ± 14.32)%; PAF: 12.01 ± 3.70 vs. 13.33 ± 1.10; t-PA: 9.75 ± 1.60 vs. 7.59 ± 2.46; Fib: 2.73 ± 0.49 vs. 3.09 ± 0.50; D-dimer: 0.233 ± 0.101 vs. 0.252 ± 0.088, all P>0.05]. CONCLUSIONS: There were changes in coagulation and fibrinolysis status in patients with AMI and UAP at the initial stage, the level of t-PA was decreased significantly. For patients with AMI and UAP, there were no obvious differences in coagulation and fibrinolysis parameters between combination therapy of clopidogrel and aspirin and aspirin alone.
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Aspirina/uso terapéutico , Enfermedad Coronaria/sangre , Fibrinólisis , Ticlopidina/análogos & derivados , Adulto , Anciano , Anciano de 80 o más Años , Angina Inestable/sangre , Angina Inestable/tratamiento farmacológico , Aspirina/administración & dosificación , Coagulación Sanguínea , Estudios de Casos y Controles , Clopidogrel , Enfermedad Coronaria/tratamiento farmacológico , Método Doble Ciego , Femenino , Productos de Degradación de Fibrina-Fibrinógeno/análisis , Humanos , Masculino , Persona de Mediana Edad , Infarto del Miocardio/sangre , Infarto del Miocardio/tratamiento farmacológico , Factor de Activación Plaquetaria/análisis , Estudios Prospectivos , Ticlopidina/administración & dosificación , Ticlopidina/uso terapéutico , Activador de Tejido Plasminógeno/sangre , Factor de von Willebrand/análisisRESUMEN
Based on software radio theory, this paper focuses on researching a direct conversion structure for the radio frequency (RF) receiver front-end and tries to apply this flexible receiver front-end in MRI receiving system. In particular, we look at the conventional architecture of RF receiver front-ends of MRI; present architecture of a direct conversion for RF receiver front-end of MRI; outline the key aspects of designing such multi-channel and multi-mode front-end systems. In addition, based on this architecture, a practical RF front-end receiving system of MRI is given. Two parts are included in this system; one is the conventional receiver front-end the other is the direct conversion design for RF receiver front-end of MRI.
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This paper introduces a novel hearing aid named artificial ultrasonic bone conduction hearing device, which is different from the traditional hearing aid in two sides: 1) sound conduction manner, 2) human perceptive principles. We focus on discussing the structure of the hearing aid and the research of frequency transposition algorithm. In addition, we design algorithm experimental platform and some sample electromechanical transducers.