RESUMEN

Nonylphenol (NP) is a persistent environmental chemical that can disrupt the organism's endocrine system, and is detected in the surface water and sea. In this study, we investigated whether NP can alter transcriptional expression of sexual differentiation-related genes. Three generations of zebrafish were exposed to 0, 2, 20 and 200 µg·L-1 of NP, and transcriptional expression of sexual differentiation genes were assessed in 10, 20 and 40 dpf in the F1 and F2 generations. Growth of zebrafish exposed to 200 µg·L-1 of NP was inhibited at 125 dpf in the F1 generation. 20 µg·L-1 of NP resulted in 80% females in the F1 generation, but had no effect on the F2 generation. In terms of the sexual differentiation genes, the transcriptional expression of cyp19a1a and esr1 genes were upregulated in 20 µg·L-1 of NP in the F1 generation. But expression of the sexual differentiation genes were not affected in the F2 generation. Overall, NP could affect sexual differentiation and gene transcriptional expression in the F1 generation. The tolerance of contaminant in the offsprings was improved at low concentration.

Asunto(s)

Disruptores Endocrinos/toxicidad , Fenoles/toxicidad , Diferenciación Sexual/efectos de los fármacos , Animales , Aromatasa/genética , Aromatasa/metabolismo , Tamaño Corporal/efectos de los fármacos , Exposición a Riesgos Ambientales , Receptor alfa de Estrógeno/genética , Receptor alfa de Estrógeno/metabolismo , Femenino , Masculino , Diferenciación Sexual/genética , Razón de Masculinidad , Regulación hacia Arriba/efectos de los fármacos , Pez Cebra/crecimiento & desarrollo , Pez Cebra/metabolismo , Proteínas de Pez Cebra/genética , Proteínas de Pez Cebra/metabolismo