RESUMEN
BACKGROUND: The purpose of this study was to compare the midterm functional and oncological outcomes of patients with distal radial tumours treated with en bloc tumour excision and osteoarticular allograft reconstruction with or without a Sauvé-Kapandji (S-K) procedure. MATERIALS AND METHOD: All cases of osteoarticular allograft reconstruction with or without the S-K procedure following resection of distal radial tumours (giant-cell tumour in 13, desmoplastic fibroma in two, osteosarcoma in one and malignant fibrous histiocytoma in one) performed from 2000 to 2009 were evaluated by clinical and radiologic examinations; the complications, functional outcomes and Musculoskeletal Tumor Society (MSTS) score were recorded. RESULTS: Seventeen patients (seven with the S-K procedure and 10 without the S-K procedure) with an average age of 31.8 years formed the study population. The follow-up time averaged 84.7 months. All patients continued to be disease-free except one with a local relapse. The reconstructive complications included non-union of radio allograft in one, allograft fracture in five, rupture of tendon in two and degenerative joint disease in all cases. Patients with the S-K procedure had better range of rotation (P = 0.040), greater grip power (P = 0.028) and less degenerative changes (P = 0.014) than those without the S-K procedure. Patients with additional S-K procedure tended to have a higher MSTS score (P = 0.073). CONCLUSIONS: En bloc resection of tumour in the distal radius followed by reconstruction with an osteoarticular allograft results in satisfactory oncologic and functional outcomes. Addition of the S-K procedure could improve functional outcomes with regard to allograft reconstruction. Evidence Rating Scale for Therapeutic Studies: Level III.
Asunto(s)
Neoplasias Óseas/cirugía , Trasplante Óseo/métodos , Fibroma Desmoplásico/cirugía , Tumor Óseo de Células Gigantes/cirugía , Osteosarcoma/cirugía , Procedimientos de Cirugía Plástica/métodos , Radio (Anatomía)/cirugía , Adolescente , Adulto , Anciano , Aloinjertos , Neoplasias Óseas/patología , Femenino , Fibroma Desmoplásico/patología , Estudios de Seguimiento , Tumor Óseo de Células Gigantes/patología , Fuerza de la Mano , Humanos , Masculino , Persona de Mediana Edad , Recurrencia Local de Neoplasia/cirugía , Estadificación de Neoplasias , Osteosarcoma/patología , Rango del Movimiento Articular , Recuperación de la Función , Estudios Retrospectivos , Adulto JovenRESUMEN
Lettuce (Lactuca sativa) as annual or biennial crop is an important vegetable in China. The lettuce variety Feiqiao, which is extensively cultivated in autumn and winter, is grown for its stem and is a characteristic species bred in Yong'an City. Since October 2005, a new disease of lettuce has been observed sporadically in the fields. Initially, chlorotic symptoms, or a faded red color, were observed on the inner leaves of the infected lettuce plants. Then, the inner leaves bleached and appeared pale, while the top leaves became straight and elongated, and stopped growing. Gummosis was observed at the base of young leaves, and the whole plant became stunted and died. The disease was named lettuce chlorotic leaf rot disease. In 2008, there was a disease outbreak in Yong'an City with an incidence of approximately 30%. In 2012, total DNA was extracted from 0.1 g of leaf tissue collected from 20 symptomatic and five asymptomatic lettuce plants using the CTAB method. A PCR analysis was performed using the phytoplasma-specific primer set R16mF2/R16mR1 (1). An approximately 1.4-kb amplicon was obtained from all 20 symptomatic plants, but no corresponding DNA fragment was amplified from the five asymptomatic plants. PCR products were cloned in Escherichia coli DH5α, using the pMD18-T vector (TaKaRa, Japan), and two isolates were sequenced. The two 1,431-bp sequences were identical (GenBank Accession No. KJ668578). A BLAST analysis revealed a 99% identity between lettuce chlorotic leaf rot phytoplasma and mulberry dwarf phytoplasma, a group 16SrI phytoplasma described by Win et al. in 2012 (3). After analyzing with iPhyclassifier, the virtual RFLP pattern derived from the 16S rDNA F2n/R2 fragment was most similar to the reference pattern of the 16Sr group I, subgroup B (NC_005303), with a pattern similarity coefficient of 0.99 (2). Additionally, the leaf veins and roots with symptoms were processed for ultrastructural examinations using transmission electron microscopy. Many typical phytoplasma-like bodies were observed in the sieve elements in the leaf veins and roots, and they were spherical to oval or dumbbell shaped and 200 to 800 nm in diameter. In agreement with these findings, seven strains of phytoplasma from 16SrI-A and 16SrI-B subgroups were reported in association with lettuce plants exhibiting various types of symptoms which were not completely consistent with those observed in China (4). To our knowledge, this is the first report of a phytoplasma associated with lettuce in China. References: (1) R. E. Davis et al. Microbiol. Res. 158:229, 2003. (2) W. Wei et al. Int. J. Syst. Evol. Microbiol. 57:1855, 2006. (3) N. K. K. Win et al. J. Gen. Plant Pathol. 78:264, 2012. (4) J. Zhang et al. Phytopathology 94:842, 2004.
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Breast cancer is one of the leading causes of cancer death in women. The Notch family of proteins plays crucial roles in determining cell fates such as proliferation, differentiation and apoptosis. A role for Notch signaling in human breast cancer has been suggested by the development of adenocarcinomas in the murine mammary gland. However, it is not clear currently whether Notch signaling is frequently expressed and activated in breast cancers. Here we show that Notch signaling is overexpressed and highly activated in breast cancers. More significantly, the attenuation of Notch signaling by gamma-secretase inhibitor can inhibit the proliferation of breast cancer cells by both causing cell cycle arrest and apoptosis. Thus, targeting Notch signaling may be of therapeutic value in breast cancers.
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Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Neoplasias de la Mama/genética , Proteínas de Unión al Calcio/genética , Proteínas de Homeodominio/genética , Péptidos y Proteínas de Señalización Intercelular/genética , Proteínas de la Membrana/genética , Receptores Notch/genética , Transducción de Señal , Proteínas Adaptadoras Transductoras de Señales , Secretasas de la Proteína Precursora del Amiloide/antagonistas & inhibidores , Secretasas de la Proteína Precursora del Amiloide/metabolismo , Apoptosis/fisiología , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/metabolismo , Mama/metabolismo , Mama/patología , Neoplasias de la Mama/metabolismo , Neoplasias de la Mama/patología , Proteínas de Unión al Calcio/metabolismo , Ciclo Celular/fisiología , Línea Celular Tumoral , Proliferación Celular , Proteínas de Homeodominio/metabolismo , Humanos , Péptidos y Proteínas de Señalización Intercelular/metabolismo , Proteínas de la Membrana/metabolismo , Inhibidores de Proteasas/farmacología , Proteínas Proto-Oncogénicas/genética , Proteínas Proto-Oncogénicas/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN Neoplásico/genética , ARN Neoplásico/metabolismo , Receptor Notch1/genética , Receptor Notch1/metabolismo , Receptor Notch3 , Receptor Notch4 , Receptores Notch/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteínas Serrate-Jagged , Factor de Transcripción HES-1 , Regulación hacia ArribaRESUMEN
The short-chain dehydrogenases/reductases (SDRs) play important roles in body's metabolism. We cloned a novel mouse SDR cDNA which encodes a deduced HSD-like protein with a conserved SDR domain and a SCP2 domain. The 1.8 kb cDNA consists of 11 exons and is mapped to mouse chromosome 4B3. The corresponding gene is widely expressed in normal mouse tissues and its expression level in liver increases after inducement with cholesterol food. The predicted mouse HSDL2 protein, which has a peroxisomal target signal, is localized in the cytoplasm of NIH 3T3 cells.